Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05785 and MMP_RS05790 are separated by 43 nucleotides MMP_RS05790 and MMP_RS05795 are separated by 48 nucleotides
MMP_RS05785: MMP_RS05785 - ATP-binding protein, at 1,109,187 to 1,111,031
_RS05785
MMP_RS05790: MMP_RS05790 - HDIG domain-containing protein, at 1,111,075 to 1,111,638
_RS05790
MMP_RS05795: MMP_RS05795 - redox-regulated ATPase YchF, at 1,111,687 to 1,112,865
_RS05795
Position (kb)
1111
1112 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1110.123 kb on + strand, within MMP_RS05785 at 1110.162 kb on + strand, within MMP_RS05785 at 1110.257 kb on - strand, within MMP_RS05785 at 1110.312 kb on + strand, within MMP_RS05785 at 1110.312 kb on + strand, within MMP_RS05785 at 1110.317 kb on - strand, within MMP_RS05785 at 1110.380 kb on + strand, within MMP_RS05785 at 1110.392 kb on - strand, within MMP_RS05785 at 1110.416 kb on + strand, within MMP_RS05785 at 1110.421 kb on + strand, within MMP_RS05785 at 1110.481 kb on - strand, within MMP_RS05785 at 1110.487 kb on - strand, within MMP_RS05785 at 1110.511 kb on - strand, within MMP_RS05785 at 1110.605 kb on + strand, within MMP_RS05785 at 1110.644 kb on - strand, within MMP_RS05785 at 1110.996 kb on + strand at 1111.024 kb on - strand at 1111.025 kb on + strand at 1111.207 kb on - strand, within MMP_RS05790 at 1111.268 kb on + strand, within MMP_RS05790 at 1111.271 kb on - strand, within MMP_RS05790 at 1111.271 kb on - strand, within MMP_RS05790 at 1111.272 kb on + strand, within MMP_RS05790 at 1111.342 kb on + strand, within MMP_RS05790 at 1111.348 kb on - strand, within MMP_RS05790 at 1111.367 kb on + strand, within MMP_RS05790 at 1111.591 kb on - strand at 1111.592 kb on + strand at 1111.603 kb on - strand at 1111.639 kb on + strand at 1111.639 kb on + strand at 1111.915 kb on - strand, within MMP_RS05795 at 1112.073 kb on + strand, within MMP_RS05795 at 1112.080 kb on - strand, within MMP_RS05795 at 1112.446 kb on + strand, within MMP_RS05795
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 1,110,123 + MMP_RS05785 0.51 -0.2 1,110,162 + MMP_RS05785 0.53 +2.4 1,110,257 - MMP_RS05785 0.58 -0.1 1,110,312 + MMP_RS05785 0.61 -0.7 1,110,312 + MMP_RS05785 0.61 -0.4 1,110,317 - MMP_RS05785 0.61 +0.8 1,110,380 + MMP_RS05785 0.65 +0.2 1,110,392 - MMP_RS05785 0.65 -0.7 1,110,416 + MMP_RS05785 0.67 -0.1 1,110,421 + MMP_RS05785 0.67 +0.1 1,110,481 - MMP_RS05785 0.70 -0.5 1,110,487 - MMP_RS05785 0.70 +1.2 1,110,511 - MMP_RS05785 0.72 +0.1 1,110,605 + MMP_RS05785 0.77 +0.2 1,110,644 - MMP_RS05785 0.79 -0.1 1,110,996 + +0.3 1,111,024 - +2.7 1,111,025 + +0.2 1,111,207 - MMP_RS05790 0.23 -0.5 1,111,268 + MMP_RS05790 0.34 +0.1 1,111,271 - MMP_RS05790 0.35 -1.4 1,111,271 - MMP_RS05790 0.35 -0.3 1,111,272 + MMP_RS05790 0.35 +0.6 1,111,342 + MMP_RS05790 0.47 -1.4 1,111,348 - MMP_RS05790 0.48 -0.2 1,111,367 + MMP_RS05790 0.52 -0.7 1,111,591 - +0.5 1,111,592 + -1.2 1,111,603 - +0.2 1,111,639 + +0.2 1,111,639 + -0.4 1,111,915 - MMP_RS05795 0.19 +3.0 1,112,073 + MMP_RS05795 0.33 +0.3 1,112,080 - MMP_RS05795 0.33 -0.2 1,112,446 + MMP_RS05795 0.64 -0.3
Or see this region's nucleotide sequence