Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05120 and MMP_RS05125 are separated by 328 nucleotides MMP_RS05125 and MMP_RS05130 are separated by 379 nucleotides
MMP_RS05120: MMP_RS05120 - DNA topoisomerase VI subunit B, at 975,268 to 977,262
_RS05120
MMP_RS05125: MMP_RS05125 - D-aminoacyl-tRNA deacylase, at 977,591 to 978,355
_RS05125
MMP_RS05130: MMP_RS05130 - DUF2226 domain-containing protein, at 978,735 to 980,321
_RS05130
Position (kb) 977
978
979 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 976.703 kb on - strand at 977.280 kb on - strand at 977.283 kb on + strand at 977.287 kb on - strand at 977.371 kb on - strand at 977.371 kb on - strand at 977.372 kb on + strand at 977.372 kb on + strand at 977.372 kb on + strand at 977.384 kb on + strand at 977.385 kb on - strand at 977.385 kb on - strand at 977.385 kb on - strand at 977.386 kb on + strand at 977.386 kb on + strand at 977.386 kb on + strand at 977.387 kb on - strand at 977.387 kb on - strand at 977.387 kb on - strand at 977.388 kb on + strand at 977.388 kb on + strand at 977.388 kb on + strand at 977.389 kb on - strand at 977.425 kb on + strand at 977.427 kb on - strand at 977.436 kb on - strand at 977.450 kb on + strand at 977.503 kb on - strand at 977.527 kb on - strand at 977.528 kb on + strand at 977.529 kb on - strand at 977.530 kb on + strand at 977.534 kb on + strand at 977.604 kb on - strand at 977.673 kb on - strand, within MMP_RS05125 at 977.673 kb on - strand, within MMP_RS05125 at 977.708 kb on - strand, within MMP_RS05125 at 977.735 kb on - strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.737 kb on - strand, within MMP_RS05125 at 977.739 kb on - strand, within MMP_RS05125 at 977.740 kb on + strand, within MMP_RS05125 at 977.740 kb on + strand, within MMP_RS05125 at 977.787 kb on - strand, within MMP_RS05125 at 977.788 kb on + strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.792 kb on + strand, within MMP_RS05125 at 977.952 kb on - strand, within MMP_RS05125 at 978.072 kb on - strand, within MMP_RS05125 at 978.137 kb on - strand, within MMP_RS05125 at 978.193 kb on + strand, within MMP_RS05125 at 978.214 kb on + strand, within MMP_RS05125 at 978.224 kb on + strand, within MMP_RS05125 at 978.299 kb on + strand at 978.389 kb on - strand at 978.390 kb on + strand at 978.393 kb on - strand at 978.394 kb on + strand at 978.394 kb on + strand at 978.396 kb on - strand at 978.408 kb on - strand at 978.409 kb on + strand at 978.423 kb on - strand at 978.423 kb on - strand at 978.423 kb on - strand at 978.424 kb on + strand at 978.424 kb on + strand at 978.435 kb on - strand at 978.439 kb on - strand at 978.439 kb on - strand at 978.446 kb on - strand at 978.447 kb on + strand at 978.630 kb on - strand at 978.630 kb on - strand at 978.631 kb on + strand at 978.633 kb on + strand at 978.702 kb on - strand at 978.703 kb on + strand at 978.721 kb on - strand at 978.745 kb on - strand at 978.745 kb on - strand at 978.745 kb on - strand at 978.745 kb on - strand at 978.746 kb on + strand at 978.746 kb on + strand at 978.777 kb on - strand at 978.874 kb on - strand at 978.882 kb on + strand at 978.909 kb on - strand, within MMP_RS05130 at 978.944 kb on + strand, within MMP_RS05130 at 979.233 kb on - strand, within MMP_RS05130 at 979.234 kb on + strand, within MMP_RS05130 at 979.286 kb on - strand, within MMP_RS05130 at 979.305 kb on + strand, within MMP_RS05130 at 979.330 kb on - strand, within MMP_RS05130 at 979.331 kb on + strand, within MMP_RS05130
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 976,703 - +0.6 977,280 - -0.0 977,283 + +2.4 977,287 - +0.3 977,371 - +0.5 977,371 - +0.3 977,372 + -0.1 977,372 + -0.9 977,372 + -0.3 977,384 + -0.5 977,385 - -0.4 977,385 - -0.3 977,385 - +0.8 977,386 + +0.3 977,386 + +4.4 977,386 + -0.7 977,387 - +0.1 977,387 - -0.6 977,387 - +0.2 977,388 + +0.6 977,388 + -1.1 977,388 + -1.1 977,389 - -0.3 977,425 + -0.4 977,427 - +0.0 977,436 - -0.2 977,450 + -1.9 977,503 - +0.1 977,527 - +0.2 977,528 + +0.4 977,529 - -0.0 977,530 + +0.1 977,534 + +0.1 977,604 - +0.8 977,673 - MMP_RS05125 0.11 -0.7 977,673 - MMP_RS05125 0.11 -0.1 977,708 - MMP_RS05125 0.15 -0.6 977,735 - MMP_RS05125 0.19 -0.6 977,736 + MMP_RS05125 0.19 +1.2 977,736 + MMP_RS05125 0.19 +1.4 977,736 + MMP_RS05125 0.19 -0.2 977,736 + MMP_RS05125 0.19 -0.5 977,737 - MMP_RS05125 0.19 +2.2 977,739 - MMP_RS05125 0.19 -0.7 977,740 + MMP_RS05125 0.19 -1.0 977,740 + MMP_RS05125 0.19 -0.1 977,787 - MMP_RS05125 0.26 -0.1 977,788 + MMP_RS05125 0.26 -0.2 977,791 - MMP_RS05125 0.26 -0.0 977,791 - MMP_RS05125 0.26 -0.0 977,791 - MMP_RS05125 0.26 -1.1 977,792 + MMP_RS05125 0.26 +2.2 977,952 - MMP_RS05125 0.47 +0.1 978,072 - MMP_RS05125 0.63 -1.9 978,137 - MMP_RS05125 0.71 +0.2 978,193 + MMP_RS05125 0.79 -0.6 978,214 + MMP_RS05125 0.81 -1.2 978,224 + MMP_RS05125 0.83 -1.1 978,299 + -0.2 978,389 - -1.0 978,390 + -0.1 978,393 - +1.0 978,394 + +0.9 978,394 + -0.3 978,396 - +0.2 978,408 - +0.0 978,409 + -1.3 978,423 - +0.6 978,423 - +0.1 978,423 - -0.1 978,424 + -1.2 978,424 + -0.1 978,435 - +0.1 978,439 - -0.1 978,439 - +1.3 978,446 - +0.0 978,447 + -0.1 978,630 - -0.2 978,630 - -0.3 978,631 + -0.1 978,633 + -0.3 978,702 - +1.0 978,703 + -0.8 978,721 - +0.3 978,745 - +0.7 978,745 - +0.1 978,745 - +0.7 978,745 - +0.1 978,746 + +0.1 978,746 + +1.1 978,777 - +0.9 978,874 - +0.6 978,882 + -0.6 978,909 - MMP_RS05130 0.11 -0.9 978,944 + MMP_RS05130 0.13 +0.7 979,233 - MMP_RS05130 0.31 -1.5 979,234 + MMP_RS05130 0.31 -0.4 979,286 - MMP_RS05130 0.35 +0.9 979,305 + MMP_RS05130 0.36 +1.5 979,330 - MMP_RS05130 0.37 +0.3 979,331 + MMP_RS05130 0.38 -0.6
Or see this region's nucleotide sequence