Strain Fitness in Methanococcus maripaludis S2 around MMP_RS05020

Experiment: H2 McCas- cells grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS05015 and MMP_RS05020 are separated by 33 nucleotidesMMP_RS05020 and MMP_RS05025 are separated by 28 nucleotides MMP_RS05015: MMP_RS05015 - histidinol dehydrogenase, at 954,600 to 955,874 _RS05015 MMP_RS05020: MMP_RS05020 - hypothetical protein, at 955,908 to 956,324 _RS05020 MMP_RS05025: MMP_RS05025 - ATP-dependent DNA ligase, at 956,353 to 958,074 _RS05025 Position (kb) 955 956 957Strain fitness (log2 ratio) -1 0 1 2at 954.979 kb on + strandat 955.060 kb on - strandat 955.394 kb on - strandat 955.394 kb on - strandat 955.401 kb on + strandat 955.554 kb on - strandat 955.927 kb on - strandat 955.932 kb on + strandat 956.053 kb on - strand, within MMP_RS05020at 956.131 kb on - strand, within MMP_RS05020at 956.174 kb on + strand, within MMP_RS05020at 956.281 kb on - strand, within MMP_RS05020at 956.281 kb on - strand, within MMP_RS05020at 956.299 kb on - strandat 956.661 kb on - strandat 956.695 kb on + strandat 956.893 kb on - strandat 957.050 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin
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954,979 + +0.4
955,060 - +1.9
955,394 - +1.1
955,394 - +2.4
955,401 + +1.1
955,554 - -0.3
955,927 - -0.2
955,932 + -0.2
956,053 - MMP_RS05020 0.35 +1.6
956,131 - MMP_RS05020 0.53 -0.1
956,174 + MMP_RS05020 0.64 -0.9
956,281 - MMP_RS05020 0.89 -0.0
956,281 - MMP_RS05020 0.89 -0.6
956,299 - +0.6
956,661 - +1.9
956,695 + +1.9
956,893 - +1.4
957,050 - +1.1

Or see this region's nucleotide sequence