Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04955 and MMP_RS04960 are separated by 1 nucleotides MMP_RS04960 and MMP_RS04965 are separated by 19 nucleotides MMP_RS04965 and MMP_RS04970 are separated by 16 nucleotides
MMP_RS04955: MMP_RS04955 - DNA topoisomerase I, at 943,765 to 946,236
_RS04955
MMP_RS04960: MMP_RS04960 - cytochrome c biogenesis CcdA family protein, at 946,238 to 946,891
_RS04960
MMP_RS04965: MMP_RS04965 - thioredoxin family protein, at 946,911 to 947,258
_RS04965
MMP_RS04970: MMP_RS04970 - F420-dependent thioredoxin reductase, at 947,275 to 948,177
_RS04970
Position (kb) 946
947
948 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 945.976 kb on + strand, within MMP_RS04955 at 946.035 kb on - strand at 946.080 kb on - strand at 946.120 kb on + strand at 946.150 kb on + strand at 946.155 kb on - strand at 946.234 kb on - strand at 946.246 kb on - strand at 946.247 kb on + strand at 946.247 kb on + strand at 946.250 kb on - strand at 946.250 kb on - strand at 946.251 kb on + strand at 946.253 kb on - strand at 946.277 kb on - strand at 946.277 kb on - strand at 946.279 kb on - strand at 946.279 kb on - strand at 946.280 kb on + strand at 946.280 kb on + strand at 946.291 kb on - strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.298 kb on - strand at 946.299 kb on + strand at 946.300 kb on - strand at 946.301 kb on + strand at 946.301 kb on + strand at 946.316 kb on - strand, within MMP_RS04960 at 946.328 kb on - strand, within MMP_RS04960 at 946.328 kb on - strand, within MMP_RS04960 at 946.479 kb on + strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.481 kb on + strand, within MMP_RS04960 at 946.539 kb on + strand, within MMP_RS04960 at 946.600 kb on - strand, within MMP_RS04960 at 946.601 kb on + strand, within MMP_RS04960 at 946.619 kb on + strand, within MMP_RS04960 at 946.619 kb on + strand, within MMP_RS04960 at 946.622 kb on - strand, within MMP_RS04960 at 946.623 kb on + strand, within MMP_RS04960 at 946.764 kb on + strand, within MMP_RS04960 at 946.766 kb on - strand, within MMP_RS04960 at 946.917 kb on + strand at 947.070 kb on - strand, within MMP_RS04965 at 947.071 kb on + strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.073 kb on + strand, within MMP_RS04965 at 947.073 kb on + strand, within MMP_RS04965 at 947.102 kb on - strand, within MMP_RS04965 at 947.201 kb on - strand, within MMP_RS04965 at 947.221 kb on + strand, within MMP_RS04965 at 947.226 kb on - strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.232 kb on - strand at 947.233 kb on + strand at 947.233 kb on + strand at 947.273 kb on - strand at 947.293 kb on - strand at 947.392 kb on + strand, within MMP_RS04970 at 947.434 kb on + strand, within MMP_RS04970 at 947.488 kb on - strand, within MMP_RS04970 at 947.635 kb on - strand, within MMP_RS04970 at 947.653 kb on - strand, within MMP_RS04970 at 947.674 kb on - strand, within MMP_RS04970 at 947.675 kb on + strand, within MMP_RS04970 at 947.675 kb on + strand, within MMP_RS04970 at 947.694 kb on - strand, within MMP_RS04970 at 947.695 kb on + strand, within MMP_RS04970 at 947.706 kb on - strand, within MMP_RS04970 at 947.706 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.786 kb on + strand, within MMP_RS04970 at 947.828 kb on + strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.863 kb on - strand, within MMP_RS04970 at 947.863 kb on - strand, within MMP_RS04970 at 947.864 kb on + strand, within MMP_RS04970 at 947.866 kb on - strand, within MMP_RS04970 at 947.883 kb on + strand, within MMP_RS04970 at 947.925 kb on - strand, within MMP_RS04970 at 947.930 kb on + strand, within MMP_RS04970 at 948.172 kb on - strand at 948.202 kb on - strand at 948.203 kb on + strand at 948.220 kb on + strand at 948.221 kb on - strand at 948.223 kb on - strand at 948.223 kb on - strand at 948.223 kb on - strand at 948.228 kb on - strand at 948.228 kb on - strand at 948.229 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 945,976 + MMP_RS04955 0.89 +1.0 946,035 - -0.5 946,080 - +1.0 946,120 + +0.1 946,150 + +0.2 946,155 - -1.4 946,234 - +0.5 946,246 - -0.5 946,247 + -1.0 946,247 + +0.7 946,250 - +0.3 946,250 - +1.9 946,251 + +0.2 946,253 - -1.0 946,277 - -0.6 946,277 - +0.4 946,279 - -0.5 946,279 - +0.9 946,280 + +1.1 946,280 + -0.6 946,291 - -0.6 946,292 + -0.0 946,292 + +0.3 946,292 + -0.5 946,292 + -1.0 946,293 - -0.4 946,293 - +0.2 946,293 - +0.7 946,293 - +0.7 946,293 - +1.2 946,293 - +1.2 946,293 - +0.7 946,294 + -0.8 946,294 + +1.0 946,294 + -0.7 946,294 + +0.1 946,294 + +1.1 946,298 - -0.6 946,299 + -0.2 946,300 - -1.3 946,301 + +0.0 946,301 + +0.3 946,316 - MMP_RS04960 0.12 -1.8 946,328 - MMP_RS04960 0.14 +0.6 946,328 - MMP_RS04960 0.14 -0.4 946,479 + MMP_RS04960 0.37 -0.2 946,480 - MMP_RS04960 0.37 +1.4 946,480 - MMP_RS04960 0.37 -0.2 946,480 - MMP_RS04960 0.37 +0.1 946,481 + MMP_RS04960 0.37 -0.2 946,539 + MMP_RS04960 0.46 +1.1 946,600 - MMP_RS04960 0.55 +0.2 946,601 + MMP_RS04960 0.56 +0.0 946,619 + MMP_RS04960 0.58 +0.6 946,619 + MMP_RS04960 0.58 -0.7 946,622 - MMP_RS04960 0.59 -0.3 946,623 + MMP_RS04960 0.59 -0.9 946,764 + MMP_RS04960 0.80 +0.3 946,766 - MMP_RS04960 0.81 -0.7 946,917 + -0.2 947,070 - MMP_RS04965 0.46 +0.1 947,071 + MMP_RS04965 0.46 -0.3 947,072 - MMP_RS04965 0.46 -0.7 947,072 - MMP_RS04965 0.46 -0.5 947,072 - MMP_RS04965 0.46 -0.5 947,072 - MMP_RS04965 0.46 +0.3 947,072 - MMP_RS04965 0.46 -1.0 947,072 - MMP_RS04965 0.46 -0.2 947,072 - MMP_RS04965 0.46 +0.7 947,073 + MMP_RS04965 0.47 +0.1 947,073 + MMP_RS04965 0.47 +0.3 947,102 - MMP_RS04965 0.55 +0.5 947,201 - MMP_RS04965 0.83 -0.7 947,221 + MMP_RS04965 0.89 -2.7 947,226 - -1.1 947,229 + -0.1 947,229 + -0.8 947,232 - -0.6 947,233 + +0.2 947,233 + -0.6 947,273 - +0.0 947,293 - -0.1 947,392 + MMP_RS04970 0.13 +0.3 947,434 + MMP_RS04970 0.18 -0.6 947,488 - MMP_RS04970 0.24 +1.1 947,635 - MMP_RS04970 0.40 +0.7 947,653 - MMP_RS04970 0.42 +0.3 947,674 - MMP_RS04970 0.44 +0.6 947,675 + MMP_RS04970 0.44 -0.2 947,675 + MMP_RS04970 0.44 +0.8 947,694 - MMP_RS04970 0.46 -0.1 947,695 + MMP_RS04970 0.47 +1.1 947,706 - MMP_RS04970 0.48 +3.4 947,706 - MMP_RS04970 0.48 +0.5 947,708 - MMP_RS04970 0.48 -0.4 947,708 - MMP_RS04970 0.48 +0.7 947,708 - MMP_RS04970 0.48 -0.9 947,786 + MMP_RS04970 0.57 +0.6 947,828 + MMP_RS04970 0.61 +0.3 947,847 - MMP_RS04970 0.63 -0.8 947,847 - MMP_RS04970 0.63 +0.0 947,847 - MMP_RS04970 0.63 -0.3 947,847 - MMP_RS04970 0.63 -0.2 947,863 - MMP_RS04970 0.65 +2.4 947,863 - MMP_RS04970 0.65 -0.9 947,864 + MMP_RS04970 0.65 -0.4 947,866 - MMP_RS04970 0.65 +1.1 947,883 + MMP_RS04970 0.67 +0.7 947,925 - MMP_RS04970 0.72 -0.4 947,930 + MMP_RS04970 0.73 -0.1 948,172 - -0.3 948,202 - -0.3 948,203 + +0.1 948,220 + -0.4 948,221 - -1.2 948,223 - -0.5 948,223 - -0.4 948,223 - +0.3 948,228 - +0.7 948,228 - +0.4 948,229 + +4.0
Or see this region's nucleotide sequence