Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04330 and MMP_RS04335 are separated by 51 nucleotides MMP_RS04335 and MMP_RS04340 are separated by 5 nucleotides MMP_RS04340 and MMP_RS04345 are separated by 68 nucleotides
MMP_RS04330: MMP_RS04330 - ASKHA domain-containing protein, at 826,046 to 827,824
_RS04330
MMP_RS04335: MMP_RS04335 - GTP-binding protein, at 827,876 to 828,784
_RS04335
MMP_RS04340: MMP_RS04340 - uroporphyrinogen decarboxylase, at 828,790 to 829,653
_RS04340
MMP_RS04345: MMP_RS04345 - MFS transporter, at 829,722 to 831,026
_RS04345
Position (kb) 828
829
830 Strain fitness (log2 ratio) -2
-1
0
1
2 at 827.800 kb on + strand at 827.830 kb on + strand at 827.833 kb on + strand at 827.841 kb on - strand at 827.844 kb on - strand at 827.851 kb on + strand at 827.862 kb on - strand at 827.873 kb on + strand at 827.895 kb on - strand at 827.896 kb on + strand at 827.929 kb on - strand at 827.930 kb on + strand at 827.931 kb on - strand at 828.077 kb on + strand, within MMP_RS04335 at 828.174 kb on - strand, within MMP_RS04335 at 828.229 kb on - strand, within MMP_RS04335 at 828.230 kb on + strand, within MMP_RS04335 at 828.240 kb on - strand, within MMP_RS04335 at 828.240 kb on - strand, within MMP_RS04335 at 828.394 kb on - strand, within MMP_RS04335 at 828.408 kb on - strand, within MMP_RS04335 at 828.409 kb on + strand, within MMP_RS04335 at 828.427 kb on - strand, within MMP_RS04335 at 828.486 kb on - strand, within MMP_RS04335 at 828.486 kb on - strand, within MMP_RS04335 at 828.487 kb on + strand, within MMP_RS04335 at 828.487 kb on + strand, within MMP_RS04335 at 828.487 kb on + strand, within MMP_RS04335 at 828.487 kb on + strand, within MMP_RS04335 at 828.489 kb on + strand, within MMP_RS04335 at 828.530 kb on + strand, within MMP_RS04335 at 828.616 kb on + strand, within MMP_RS04335 at 828.665 kb on + strand, within MMP_RS04335 at 828.665 kb on + strand, within MMP_RS04335 at 828.668 kb on - strand, within MMP_RS04335 at 828.760 kb on + strand at 829.058 kb on - strand, within MMP_RS04340 at 829.059 kb on + strand, within MMP_RS04340 at 829.065 kb on - strand, within MMP_RS04340 at 829.066 kb on + strand, within MMP_RS04340 at 829.082 kb on + strand, within MMP_RS04340 at 829.084 kb on - strand, within MMP_RS04340 at 829.084 kb on - strand, within MMP_RS04340 at 829.084 kb on - strand, within MMP_RS04340 at 829.084 kb on - strand, within MMP_RS04340 at 829.084 kb on - strand, within MMP_RS04340 at 829.085 kb on + strand, within MMP_RS04340 at 829.085 kb on + strand, within MMP_RS04340 at 829.092 kb on - strand, within MMP_RS04340 at 829.154 kb on - strand, within MMP_RS04340 at 829.154 kb on - strand, within MMP_RS04340 at 829.154 kb on - strand, within MMP_RS04340 at 829.155 kb on + strand, within MMP_RS04340 at 829.155 kb on + strand, within MMP_RS04340 at 829.155 kb on + strand, within MMP_RS04340 at 829.155 kb on + strand, within MMP_RS04340 at 829.320 kb on - strand, within MMP_RS04340 at 829.545 kb on - strand, within MMP_RS04340 at 829.545 kb on - strand, within MMP_RS04340 at 829.546 kb on + strand, within MMP_RS04340 at 829.549 kb on - strand, within MMP_RS04340 at 829.550 kb on + strand, within MMP_RS04340 at 829.550 kb on + strand, within MMP_RS04340 at 829.669 kb on - strand at 829.724 kb on + strand at 829.785 kb on - strand at 829.786 kb on + strand at 829.836 kb on + strand at 829.948 kb on + strand, within MMP_RS04345 at 829.967 kb on + strand, within MMP_RS04345 at 829.971 kb on - strand, within MMP_RS04345 at 829.971 kb on - strand, within MMP_RS04345 at 829.972 kb on + strand, within MMP_RS04345 at 829.972 kb on + strand, within MMP_RS04345 at 829.972 kb on + strand, within MMP_RS04345 at 830.008 kb on - strand, within MMP_RS04345 at 830.008 kb on - strand, within MMP_RS04345 at 830.009 kb on + strand, within MMP_RS04345 at 830.018 kb on - strand, within MMP_RS04345 at 830.157 kb on - strand, within MMP_RS04345 at 830.197 kb on - strand, within MMP_RS04345 at 830.322 kb on - strand, within MMP_RS04345 at 830.323 kb on + strand, within MMP_RS04345 at 830.326 kb on + strand, within MMP_RS04345 at 830.353 kb on + strand, within MMP_RS04345 at 830.461 kb on + strand, within MMP_RS04345 at 830.461 kb on + strand, within MMP_RS04345 at 830.494 kb on + strand, within MMP_RS04345 at 830.507 kb on - strand, within MMP_RS04345 at 830.511 kb on - strand, within MMP_RS04345 at 830.527 kb on + strand, within MMP_RS04345 at 830.527 kb on + strand, within MMP_RS04345 at 830.597 kb on - strand, within MMP_RS04345
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 827,800 + -0.8 827,830 + -0.2 827,833 + +0.0 827,841 - -1.4 827,844 - +0.0 827,851 + +0.8 827,862 - -0.2 827,873 + -1.3 827,895 - +0.6 827,896 + -0.7 827,929 - +0.5 827,930 + -0.1 827,931 - -0.9 828,077 + MMP_RS04335 0.22 +0.1 828,174 - MMP_RS04335 0.33 -0.6 828,229 - MMP_RS04335 0.39 -0.3 828,230 + MMP_RS04335 0.39 +2.0 828,240 - MMP_RS04335 0.40 +0.0 828,240 - MMP_RS04335 0.40 +1.1 828,394 - MMP_RS04335 0.57 -1.1 828,408 - MMP_RS04335 0.59 +0.1 828,409 + MMP_RS04335 0.59 -0.6 828,427 - MMP_RS04335 0.61 -0.6 828,486 - MMP_RS04335 0.67 -0.2 828,486 - MMP_RS04335 0.67 -1.0 828,487 + MMP_RS04335 0.67 +0.6 828,487 + MMP_RS04335 0.67 -0.1 828,487 + MMP_RS04335 0.67 -0.2 828,487 + MMP_RS04335 0.67 -0.8 828,489 + MMP_RS04335 0.67 -0.4 828,530 + MMP_RS04335 0.72 -0.0 828,616 + MMP_RS04335 0.81 -0.4 828,665 + MMP_RS04335 0.87 -0.9 828,665 + MMP_RS04335 0.87 +0.4 828,668 - MMP_RS04335 0.87 +1.9 828,760 + -1.2 829,058 - MMP_RS04340 0.31 -0.1 829,059 + MMP_RS04340 0.31 +0.1 829,065 - MMP_RS04340 0.32 -0.2 829,066 + MMP_RS04340 0.32 -1.1 829,082 + MMP_RS04340 0.34 +0.7 829,084 - MMP_RS04340 0.34 -2.0 829,084 - MMP_RS04340 0.34 -0.5 829,084 - MMP_RS04340 0.34 -0.5 829,084 - MMP_RS04340 0.34 -0.4 829,084 - MMP_RS04340 0.34 -0.3 829,085 + MMP_RS04340 0.34 +0.2 829,085 + MMP_RS04340 0.34 -0.3 829,092 - MMP_RS04340 0.35 -0.1 829,154 - MMP_RS04340 0.42 -0.2 829,154 - MMP_RS04340 0.42 -0.3 829,154 - MMP_RS04340 0.42 -0.2 829,155 + MMP_RS04340 0.42 -1.5 829,155 + MMP_RS04340 0.42 -1.0 829,155 + MMP_RS04340 0.42 +0.5 829,155 + MMP_RS04340 0.42 -0.2 829,320 - MMP_RS04340 0.61 +0.0 829,545 - MMP_RS04340 0.87 +0.2 829,545 - MMP_RS04340 0.87 -1.0 829,546 + MMP_RS04340 0.88 +0.9 829,549 - MMP_RS04340 0.88 +0.8 829,550 + MMP_RS04340 0.88 -0.2 829,550 + MMP_RS04340 0.88 -0.2 829,669 - -0.5 829,724 + +0.2 829,785 - -0.3 829,786 + +0.8 829,836 + +0.9 829,948 + MMP_RS04345 0.17 -0.4 829,967 + MMP_RS04345 0.19 -1.2 829,971 - MMP_RS04345 0.19 +2.5 829,971 - MMP_RS04345 0.19 +0.9 829,972 + MMP_RS04345 0.19 +0.0 829,972 + MMP_RS04345 0.19 -1.0 829,972 + MMP_RS04345 0.19 -1.0 830,008 - MMP_RS04345 0.22 -0.7 830,008 - MMP_RS04345 0.22 -0.2 830,009 + MMP_RS04345 0.22 +0.2 830,018 - MMP_RS04345 0.23 -0.6 830,157 - MMP_RS04345 0.33 +0.0 830,197 - MMP_RS04345 0.36 +1.1 830,322 - MMP_RS04345 0.46 +0.2 830,323 + MMP_RS04345 0.46 -0.3 830,326 + MMP_RS04345 0.46 -0.6 830,353 + MMP_RS04345 0.48 -0.1 830,461 + MMP_RS04345 0.57 -0.3 830,461 + MMP_RS04345 0.57 -0.1 830,494 + MMP_RS04345 0.59 -1.2 830,507 - MMP_RS04345 0.60 +0.7 830,511 - MMP_RS04345 0.60 -0.0 830,527 + MMP_RS04345 0.62 -0.9 830,527 + MMP_RS04345 0.62 -0.4 830,597 - MMP_RS04345 0.67 +0.0
Or see this region's nucleotide sequence