Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04185 and MMP_RS04190 are separated by 87 nucleotides MMP_RS04190 and MMP_RS04195 are separated by 217 nucleotides MMP_RS04195 and MMP_RS04200 are separated by 30 nucleotides
MMP_RS04185: MMP_RS04185 - aldo/keto reductase, at 796,127 to 797,239
_RS04185
MMP_RS04190: MMP_RS04190 - hypothetical protein, at 797,327 to 797,842
_RS04190
MMP_RS04195: MMP_RS04195 - flavodoxin, at 798,060 to 798,596
_RS04195
MMP_RS04200: MMP_RS04200 - DUF111 family protein, at 798,627 to 799,346
_RS04200
Position (kb) 797
798 Strain fitness (log2 ratio) -2
-1
0
1
2 at 796.333 kb on - strand, within MMP_RS04185 at 796.333 kb on - strand, within MMP_RS04185 at 796.347 kb on - strand, within MMP_RS04185 at 796.347 kb on - strand, within MMP_RS04185 at 796.445 kb on + strand, within MMP_RS04185 at 796.448 kb on + strand, within MMP_RS04185 at 796.600 kb on - strand, within MMP_RS04185 at 796.635 kb on - strand, within MMP_RS04185 at 796.861 kb on + strand, within MMP_RS04185 at 796.881 kb on - strand, within MMP_RS04185 at 796.916 kb on - strand, within MMP_RS04185 at 796.919 kb on - strand, within MMP_RS04185 at 797.002 kb on - strand, within MMP_RS04185 at 797.047 kb on + strand, within MMP_RS04185 at 797.055 kb on + strand, within MMP_RS04185 at 797.099 kb on - strand, within MMP_RS04185 at 797.100 kb on + strand, within MMP_RS04185 at 797.172 kb on - strand at 797.273 kb on - strand at 797.305 kb on + strand at 797.361 kb on - strand at 797.362 kb on + strand at 797.362 kb on + strand at 797.362 kb on + strand at 797.365 kb on + strand at 797.403 kb on + strand, within MMP_RS04190 at 797.536 kb on + strand, within MMP_RS04190 at 797.597 kb on + strand, within MMP_RS04190 at 797.709 kb on + strand, within MMP_RS04190 at 797.723 kb on - strand, within MMP_RS04190 at 797.728 kb on - strand, within MMP_RS04190 at 797.728 kb on - strand, within MMP_RS04190 at 797.810 kb on + strand at 797.810 kb on + strand at 797.818 kb on - strand at 797.821 kb on + strand at 797.821 kb on + strand at 797.859 kb on - strand at 797.959 kb on + strand at 797.959 kb on + strand at 798.002 kb on + strand at 798.090 kb on + strand at 798.090 kb on + strand at 798.179 kb on + strand, within MMP_RS04195 at 798.301 kb on - strand, within MMP_RS04195 at 798.508 kb on - strand, within MMP_RS04195 at 798.511 kb on - strand, within MMP_RS04195 at 798.574 kb on - strand at 798.578 kb on - strand at 798.579 kb on + strand at 798.608 kb on + strand at 798.612 kb on + strand at 798.622 kb on - strand at 798.623 kb on + strand at 798.639 kb on + strand at 798.687 kb on + strand at 798.687 kb on + strand at 798.743 kb on + strand, within MMP_RS04200 at 798.759 kb on + strand, within MMP_RS04200 at 798.782 kb on - strand, within MMP_RS04200 at 798.806 kb on - strand, within MMP_RS04200 at 798.807 kb on + strand, within MMP_RS04200 at 798.837 kb on - strand, within MMP_RS04200
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 796,333 - MMP_RS04185 0.19 -0.7 796,333 - MMP_RS04185 0.19 +1.8 796,347 - MMP_RS04185 0.20 -0.2 796,347 - MMP_RS04185 0.20 -0.2 796,445 + MMP_RS04185 0.29 +0.1 796,448 + MMP_RS04185 0.29 -0.1 796,600 - MMP_RS04185 0.42 +0.5 796,635 - MMP_RS04185 0.46 -0.4 796,861 + MMP_RS04185 0.66 -1.2 796,881 - MMP_RS04185 0.68 -2.5 796,916 - MMP_RS04185 0.71 +0.1 796,919 - MMP_RS04185 0.71 +0.8 797,002 - MMP_RS04185 0.79 +0.1 797,047 + MMP_RS04185 0.83 +0.2 797,055 + MMP_RS04185 0.83 -0.4 797,099 - MMP_RS04185 0.87 -0.7 797,100 + MMP_RS04185 0.87 +1.0 797,172 - +0.7 797,273 - -0.6 797,305 + -1.1 797,361 - -0.8 797,362 + +0.4 797,362 + -0.5 797,362 + -0.2 797,365 + -0.4 797,403 + MMP_RS04190 0.15 +0.2 797,536 + MMP_RS04190 0.41 -0.2 797,597 + MMP_RS04190 0.52 -0.4 797,709 + MMP_RS04190 0.74 -0.2 797,723 - MMP_RS04190 0.77 +0.1 797,728 - MMP_RS04190 0.78 -0.5 797,728 - MMP_RS04190 0.78 -2.0 797,810 + +0.7 797,810 + +0.1 797,818 - +1.1 797,821 + +0.8 797,821 + +0.1 797,859 - +0.2 797,959 + +0.5 797,959 + -2.2 798,002 + -0.0 798,090 + +0.1 798,090 + -0.6 798,179 + MMP_RS04195 0.22 +0.7 798,301 - MMP_RS04195 0.45 -1.1 798,508 - MMP_RS04195 0.83 -1.3 798,511 - MMP_RS04195 0.84 +0.0 798,574 - +0.2 798,578 - -0.3 798,579 + +0.4 798,608 + -0.2 798,612 + -0.2 798,622 - -0.5 798,623 + -0.2 798,639 + -1.2 798,687 + +1.1 798,687 + +0.3 798,743 + MMP_RS04200 0.16 +0.4 798,759 + MMP_RS04200 0.18 -0.2 798,782 - MMP_RS04200 0.22 +1.1 798,806 - MMP_RS04200 0.25 -0.1 798,807 + MMP_RS04200 0.25 +1.4 798,837 - MMP_RS04200 0.29 +1.5
Or see this region's nucleotide sequence