Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08885 and MMP_RS03975 are separated by 111 nucleotides MMP_RS03975 and MMP_RS03980 are separated by 31 nucleotides MMP_RS03980 and MMP_RS03985 are separated by 118 nucleotides MMP_RS03985 and MMP_RS03990 are separated by 944 nucleotides
MMP_RS08885: MMP_RS08885 - tetratricopeptide repeat protein, at 753,138 to 753,662
_RS08885
MMP_RS03975: MMP_RS03975 - hypothetical protein, at 753,774 to 754,082
_RS03975
MMP_RS03980: MMP_RS03980 - hypothetical protein, at 754,114 to 754,386
_RS03980
MMP_RS03985: MMP_RS03985 - hypothetical protein, at 754,505 to 755,509
_RS03985
MMP_RS03990: MMP_RS03990 - recombinase family protein, at 756,454 to 757,008
_RS03990
Position (kb) 754
755
756 Strain fitness (log2 ratio) -2
-1
0
1
2 at 753.546 kb on + strand, within MMP_RS08885 at 753.546 kb on + strand, within MMP_RS08885 at 753.549 kb on - strand, within MMP_RS08885 at 753.549 kb on - strand, within MMP_RS08885 at 753.549 kb on - strand, within MMP_RS08885 at 753.550 kb on + strand, within MMP_RS08885 at 753.550 kb on + strand, within MMP_RS08885 at 753.553 kb on + strand, within MMP_RS08885 at 753.569 kb on - strand, within MMP_RS08885 at 753.570 kb on + strand, within MMP_RS08885 at 753.600 kb on - strand, within MMP_RS08885 at 753.608 kb on + strand, within MMP_RS08885 at 753.624 kb on - strand at 753.658 kb on - strand at 753.661 kb on + strand at 753.682 kb on + strand at 753.709 kb on - strand at 753.740 kb on + strand at 753.747 kb on + strand at 753.785 kb on - strand at 753.809 kb on - strand, within MMP_RS03975 at 753.810 kb on + strand, within MMP_RS03975 at 753.867 kb on + strand, within MMP_RS03975 at 753.869 kb on - strand, within MMP_RS03975 at 753.870 kb on + strand, within MMP_RS03975 at 753.950 kb on + strand, within MMP_RS03975 at 753.957 kb on + strand, within MMP_RS03975 at 753.967 kb on + strand, within MMP_RS03975 at 753.997 kb on - strand, within MMP_RS03975 at 753.997 kb on - strand, within MMP_RS03975 at 753.998 kb on + strand, within MMP_RS03975 at 754.000 kb on + strand, within MMP_RS03975 at 754.015 kb on - strand, within MMP_RS03975 at 754.021 kb on - strand, within MMP_RS03975 at 754.021 kb on - strand, within MMP_RS03975 at 754.022 kb on + strand, within MMP_RS03975 at 754.022 kb on + strand, within MMP_RS03975 at 754.023 kb on - strand, within MMP_RS03975 at 754.023 kb on - strand, within MMP_RS03975 at 754.023 kb on - strand, within MMP_RS03975 at 754.023 kb on - strand, within MMP_RS03975 at 754.023 kb on - strand, within MMP_RS03975 at 754.024 kb on + strand, within MMP_RS03975 at 754.025 kb on - strand, within MMP_RS03975 at 754.026 kb on + strand, within MMP_RS03975 at 754.026 kb on + strand, within MMP_RS03975 at 754.026 kb on + strand, within MMP_RS03975 at 754.070 kb on + strand at 754.073 kb on + strand at 754.177 kb on - strand, within MMP_RS03980 at 754.228 kb on + strand, within MMP_RS03980 at 754.241 kb on + strand, within MMP_RS03980 at 754.264 kb on + strand, within MMP_RS03980 at 754.266 kb on + strand, within MMP_RS03980 at 754.284 kb on - strand, within MMP_RS03980 at 754.299 kb on - strand, within MMP_RS03980 at 754.305 kb on + strand, within MMP_RS03980 at 754.305 kb on + strand, within MMP_RS03980 at 754.323 kb on - strand, within MMP_RS03980 at 754.323 kb on - strand, within MMP_RS03980 at 754.347 kb on + strand, within MMP_RS03980 at 754.408 kb on - strand at 754.502 kb on - strand at 754.541 kb on + strand at 754.594 kb on - strand at 754.597 kb on - strand at 754.601 kb on - strand at 754.635 kb on + strand, within MMP_RS03985 at 754.707 kb on + strand, within MMP_RS03985 at 754.883 kb on + strand, within MMP_RS03985 at 754.886 kb on - strand, within MMP_RS03985 at 754.893 kb on + strand, within MMP_RS03985 at 754.938 kb on + strand, within MMP_RS03985 at 754.974 kb on - strand, within MMP_RS03985 at 754.975 kb on + strand, within MMP_RS03985 at 755.024 kb on - strand, within MMP_RS03985 at 755.025 kb on + strand, within MMP_RS03985 at 755.051 kb on + strand, within MMP_RS03985 at 755.126 kb on - strand, within MMP_RS03985 at 755.157 kb on - strand, within MMP_RS03985 at 755.163 kb on - strand, within MMP_RS03985 at 755.163 kb on - strand, within MMP_RS03985 at 755.164 kb on + strand, within MMP_RS03985 at 755.164 kb on + strand, within MMP_RS03985 at 755.180 kb on - strand, within MMP_RS03985 at 755.180 kb on - strand, within MMP_RS03985 at 755.181 kb on + strand, within MMP_RS03985 at 755.284 kb on - strand, within MMP_RS03985 at 755.287 kb on - strand, within MMP_RS03985 at 755.325 kb on - strand, within MMP_RS03985 at 755.371 kb on - strand, within MMP_RS03985 at 755.454 kb on - strand at 755.498 kb on + strand at 755.512 kb on - strand at 755.643 kb on - strand at 755.645 kb on - strand at 755.645 kb on - strand at 755.646 kb on + strand at 755.648 kb on + strand at 755.648 kb on + strand at 755.648 kb on + strand at 755.665 kb on - strand at 755.708 kb on - strand at 755.708 kb on - strand at 755.709 kb on + strand at 755.709 kb on + strand at 755.709 kb on + strand at 755.709 kb on + strand at 755.710 kb on - strand at 755.711 kb on + strand at 755.794 kb on - strand at 755.836 kb on - strand at 755.836 kb on - strand at 755.836 kb on - strand at 755.837 kb on + strand at 755.837 kb on + strand at 755.859 kb on - strand at 755.860 kb on + strand at 755.892 kb on + strand at 755.937 kb on + strand at 755.937 kb on + strand at 755.955 kb on - strand at 755.956 kb on + strand at 755.989 kb on - strand at 755.990 kb on + strand at 755.991 kb on - strand at 755.995 kb on - strand at 755.996 kb on + strand at 755.998 kb on - strand at 755.998 kb on - strand at 756.074 kb on - strand at 756.093 kb on - strand at 756.093 kb on - strand at 756.109 kb on - strand at 756.118 kb on + strand at 756.143 kb on - strand at 756.147 kb on - strand at 756.148 kb on + strand at 756.203 kb on + strand at 756.209 kb on + strand at 756.267 kb on - strand at 756.305 kb on + strand at 756.339 kb on + strand at 756.371 kb on + strand at 756.391 kb on - strand at 756.392 kb on + strand at 756.455 kb on - strand at 756.455 kb on - strand at 756.456 kb on + strand at 756.473 kb on - strand at 756.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 753,546 + MMP_RS08885 0.78 +0.1 753,546 + MMP_RS08885 0.78 +0.8 753,549 - MMP_RS08885 0.78 -2.0 753,549 - MMP_RS08885 0.78 -1.3 753,549 - MMP_RS08885 0.78 -0.2 753,550 + MMP_RS08885 0.78 +1.6 753,550 + MMP_RS08885 0.78 -0.2 753,553 + MMP_RS08885 0.79 -1.2 753,569 - MMP_RS08885 0.82 +0.1 753,570 + MMP_RS08885 0.82 +0.7 753,600 - MMP_RS08885 0.88 -0.4 753,608 + MMP_RS08885 0.90 -0.2 753,624 - +0.7 753,658 - +1.4 753,661 + -0.7 753,682 + +0.4 753,709 - -0.1 753,740 + +0.1 753,747 + +0.6 753,785 - -1.0 753,809 - MMP_RS03975 0.11 +0.4 753,810 + MMP_RS03975 0.12 +0.5 753,867 + MMP_RS03975 0.30 -0.6 753,869 - MMP_RS03975 0.31 -0.3 753,870 + MMP_RS03975 0.31 +0.1 753,950 + MMP_RS03975 0.57 -0.4 753,957 + MMP_RS03975 0.59 -0.5 753,967 + MMP_RS03975 0.62 -0.4 753,997 - MMP_RS03975 0.72 -0.8 753,997 - MMP_RS03975 0.72 +0.2 753,998 + MMP_RS03975 0.72 +0.2 754,000 + MMP_RS03975 0.73 -0.2 754,015 - MMP_RS03975 0.78 -0.6 754,021 - MMP_RS03975 0.80 -1.8 754,021 - MMP_RS03975 0.80 +0.4 754,022 + MMP_RS03975 0.80 -0.1 754,022 + MMP_RS03975 0.80 +0.1 754,023 - MMP_RS03975 0.81 +1.1 754,023 - MMP_RS03975 0.81 -0.9 754,023 - MMP_RS03975 0.81 +1.2 754,023 - MMP_RS03975 0.81 -2.5 754,023 - MMP_RS03975 0.81 +1.0 754,024 + MMP_RS03975 0.81 +0.1 754,025 - MMP_RS03975 0.81 +1.0 754,026 + MMP_RS03975 0.82 +1.1 754,026 + MMP_RS03975 0.82 +0.4 754,026 + MMP_RS03975 0.82 +1.6 754,070 + -0.8 754,073 + +0.1 754,177 - MMP_RS03980 0.23 +0.0 754,228 + MMP_RS03980 0.42 -0.4 754,241 + MMP_RS03980 0.47 -1.8 754,264 + MMP_RS03980 0.55 -0.1 754,266 + MMP_RS03980 0.56 +0.4 754,284 - MMP_RS03980 0.62 +1.9 754,299 - MMP_RS03980 0.68 -0.4 754,305 + MMP_RS03980 0.70 +0.0 754,305 + MMP_RS03980 0.70 +0.3 754,323 - MMP_RS03980 0.77 +0.5 754,323 - MMP_RS03980 0.77 +0.3 754,347 + MMP_RS03980 0.85 -0.8 754,408 - -1.1 754,502 - -0.7 754,541 + +1.1 754,594 - -1.4 754,597 - +0.0 754,601 - +0.7 754,635 + MMP_RS03985 0.13 +0.2 754,707 + MMP_RS03985 0.20 +0.1 754,883 + MMP_RS03985 0.38 -0.2 754,886 - MMP_RS03985 0.38 +0.5 754,893 + MMP_RS03985 0.39 -0.2 754,938 + MMP_RS03985 0.43 -0.4 754,974 - MMP_RS03985 0.47 +0.2 754,975 + MMP_RS03985 0.47 -0.7 755,024 - MMP_RS03985 0.52 -1.1 755,025 + MMP_RS03985 0.52 -1.7 755,051 + MMP_RS03985 0.54 +0.1 755,126 - MMP_RS03985 0.62 -1.0 755,157 - MMP_RS03985 0.65 +0.1 755,163 - MMP_RS03985 0.65 -0.9 755,163 - MMP_RS03985 0.65 -1.0 755,164 + MMP_RS03985 0.66 -0.1 755,164 + MMP_RS03985 0.66 +0.4 755,180 - MMP_RS03985 0.67 -0.3 755,180 - MMP_RS03985 0.67 -0.6 755,181 + MMP_RS03985 0.67 -1.1 755,284 - MMP_RS03985 0.78 -0.1 755,287 - MMP_RS03985 0.78 +0.1 755,325 - MMP_RS03985 0.82 +1.1 755,371 - MMP_RS03985 0.86 +1.3 755,454 - +1.0 755,498 + -0.7 755,512 - -0.3 755,643 - +0.5 755,645 - -0.6 755,645 - -0.7 755,646 + +0.2 755,648 + +0.0 755,648 + -0.1 755,648 + +0.6 755,665 - -0.4 755,708 - -0.0 755,708 - -0.3 755,709 + +0.1 755,709 + +0.6 755,709 + +0.0 755,709 + +1.6 755,710 - +0.3 755,711 + +0.1 755,794 - -0.2 755,836 - -0.6 755,836 - -0.7 755,836 - +0.3 755,837 + +0.6 755,837 + +2.3 755,859 - -0.1 755,860 + +0.4 755,892 + +0.5 755,937 + +1.3 755,937 + -1.7 755,955 - +0.0 755,956 + +0.5 755,989 - -0.6 755,990 + -0.5 755,991 - +0.5 755,995 - +0.1 755,996 + -0.6 755,998 - -0.5 755,998 - -0.4 756,074 - +0.5 756,093 - -0.0 756,093 - +0.6 756,109 - +0.4 756,118 + -0.5 756,143 - -0.9 756,147 - -0.4 756,148 + -0.8 756,203 + +1.4 756,209 + -0.3 756,267 - -0.5 756,305 + -1.2 756,339 + +0.9 756,371 + +0.5 756,391 - -0.8 756,392 + -0.1 756,455 - +0.2 756,455 - +1.5 756,456 + -1.4 756,473 - -0.2 756,477 - +0.7
Or see this region's nucleotide sequence