Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03835 and MMP_RS03840 are separated by 136 nucleotides MMP_RS03840 and MMP_RS03845 are separated by 74 nucleotides
MMP_RS03835: MMP_RS03835 - MATE family efflux transporter, at 725,998 to 727,350
_RS03835
MMP_RS03840: MMP_RS03840 - hypothetical protein, at 727,487 to 727,804
_RS03840
MMP_RS03845: MMP_RS03845 - DNA adenine methylase, at 727,879 to 728,799
_RS03845
Position (kb)
727
728 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 726.494 kb on - strand, within MMP_RS03835 at 726.513 kb on + strand, within MMP_RS03835 at 726.719 kb on + strand, within MMP_RS03835 at 726.725 kb on + strand, within MMP_RS03835 at 726.956 kb on - strand, within MMP_RS03835 at 726.957 kb on + strand, within MMP_RS03835 at 726.985 kb on + strand, within MMP_RS03835 at 727.027 kb on + strand, within MMP_RS03835 at 727.045 kb on - strand, within MMP_RS03835 at 727.045 kb on - strand, within MMP_RS03835 at 727.052 kb on - strand, within MMP_RS03835 at 727.052 kb on - strand, within MMP_RS03835 at 727.052 kb on - strand, within MMP_RS03835 at 727.053 kb on + strand, within MMP_RS03835 at 727.053 kb on + strand, within MMP_RS03835 at 727.058 kb on + strand, within MMP_RS03835 at 727.070 kb on + strand, within MMP_RS03835 at 727.120 kb on - strand, within MMP_RS03835 at 727.147 kb on + strand, within MMP_RS03835 at 727.161 kb on - strand, within MMP_RS03835 at 727.234 kb on - strand at 727.263 kb on - strand at 727.266 kb on - strand at 727.314 kb on - strand at 727.314 kb on - strand at 727.315 kb on + strand at 727.350 kb on - strand at 727.382 kb on + strand at 727.391 kb on - strand at 727.429 kb on - strand at 727.429 kb on - strand at 727.429 kb on - strand at 727.430 kb on + strand at 727.431 kb on - strand at 727.431 kb on - strand at 727.431 kb on - strand at 727.431 kb on - strand at 727.431 kb on - strand at 727.432 kb on + strand at 727.432 kb on + strand at 727.432 kb on + strand at 727.433 kb on - strand at 727.433 kb on - strand at 727.433 kb on - strand at 727.433 kb on - strand at 727.433 kb on - strand at 727.434 kb on + strand at 727.434 kb on + strand at 727.434 kb on + strand at 727.434 kb on + strand at 727.468 kb on - strand at 727.469 kb on + strand at 727.469 kb on + strand at 727.469 kb on + strand at 727.484 kb on - strand at 727.485 kb on + strand at 727.485 kb on + strand at 727.485 kb on + strand at 727.485 kb on + strand at 727.486 kb on - strand at 727.486 kb on - strand at 727.486 kb on - strand at 727.497 kb on + strand at 727.528 kb on + strand, within MMP_RS03840 at 727.564 kb on - strand, within MMP_RS03840 at 727.656 kb on - strand, within MMP_RS03840 at 727.781 kb on + strand at 727.782 kb on - strand at 727.810 kb on - strand at 727.848 kb on + strand at 727.873 kb on - strand at 727.878 kb on - strand at 727.879 kb on + strand at 728.024 kb on + strand, within MMP_RS03845 at 728.075 kb on - strand, within MMP_RS03845 at 728.198 kb on + strand, within MMP_RS03845 at 728.219 kb on + strand, within MMP_RS03845 at 728.323 kb on + strand, within MMP_RS03845 at 728.382 kb on + strand, within MMP_RS03845 at 728.472 kb on + strand, within MMP_RS03845 at 728.583 kb on + strand, within MMP_RS03845 at 728.602 kb on + strand, within MMP_RS03845 at 728.626 kb on - strand, within MMP_RS03845 at 728.627 kb on + strand, within MMP_RS03845 at 728.627 kb on + strand, within MMP_RS03845 at 728.640 kb on - strand, within MMP_RS03845 at 728.641 kb on + strand, within MMP_RS03845 at 728.658 kb on - strand, within MMP_RS03845 at 728.704 kb on + strand, within MMP_RS03845 at 728.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 726,494 - MMP_RS03835 0.37 -1.9 726,513 + MMP_RS03835 0.38 +0.7 726,719 + MMP_RS03835 0.53 -0.8 726,725 + MMP_RS03835 0.54 +0.5 726,956 - MMP_RS03835 0.71 +0.4 726,957 + MMP_RS03835 0.71 +0.5 726,985 + MMP_RS03835 0.73 +0.9 727,027 + MMP_RS03835 0.76 +0.2 727,045 - MMP_RS03835 0.77 +0.7 727,045 - MMP_RS03835 0.77 +0.3 727,052 - MMP_RS03835 0.78 +3.5 727,052 - MMP_RS03835 0.78 -0.2 727,052 - MMP_RS03835 0.78 +0.6 727,053 + MMP_RS03835 0.78 -1.0 727,053 + MMP_RS03835 0.78 -0.9 727,058 + MMP_RS03835 0.78 -0.9 727,070 + MMP_RS03835 0.79 +0.2 727,120 - MMP_RS03835 0.83 -0.8 727,147 + MMP_RS03835 0.85 -0.5 727,161 - MMP_RS03835 0.86 -2.1 727,234 - -1.1 727,263 - +0.2 727,266 - +0.1 727,314 - -2.0 727,314 - +0.7 727,315 + +1.7 727,350 - +2.8 727,382 + -0.6 727,391 - -1.1 727,429 - +0.3 727,429 - +1.0 727,429 - -0.1 727,430 + -0.1 727,431 - +1.0 727,431 - +0.0 727,431 - -1.5 727,431 - +0.5 727,431 - +0.2 727,432 + +0.7 727,432 + +0.2 727,432 + +0.1 727,433 - +0.1 727,433 - -1.1 727,433 - -0.5 727,433 - +0.0 727,433 - -0.2 727,434 + -1.2 727,434 + +1.0 727,434 + +1.6 727,434 + -0.6 727,468 - -1.0 727,469 + -0.1 727,469 + +0.2 727,469 + -0.2 727,484 - +1.5 727,485 + -1.2 727,485 + -0.6 727,485 + -1.3 727,485 + +0.8 727,486 - +0.3 727,486 - +0.3 727,486 - +0.3 727,497 + -0.4 727,528 + MMP_RS03840 0.13 +0.2 727,564 - MMP_RS03840 0.24 -0.9 727,656 - MMP_RS03840 0.53 -1.0 727,781 + +0.4 727,782 - +0.5 727,810 - +0.6 727,848 + -0.6 727,873 - +0.1 727,878 - +0.1 727,879 + -1.1 728,024 + MMP_RS03845 0.16 -1.0 728,075 - MMP_RS03845 0.21 -1.0 728,198 + MMP_RS03845 0.35 -0.3 728,219 + MMP_RS03845 0.37 +0.9 728,323 + MMP_RS03845 0.48 +0.0 728,382 + MMP_RS03845 0.55 -0.5 728,472 + MMP_RS03845 0.64 -0.5 728,583 + MMP_RS03845 0.76 +0.4 728,602 + MMP_RS03845 0.79 +0.0 728,626 - MMP_RS03845 0.81 +0.7 728,627 + MMP_RS03845 0.81 +0.3 728,627 + MMP_RS03845 0.81 +0.2 728,640 - MMP_RS03845 0.83 +1.0 728,641 + MMP_RS03845 0.83 +0.2 728,658 - MMP_RS03845 0.85 -0.7 728,704 + MMP_RS03845 0.90 +0.8 728,777 - +0.2
Or see this region's nucleotide sequence