Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02825 and MMP_RS02830 are separated by 0 nucleotides MMP_RS02830 and MMP_RS02835 are separated by 148 nucleotides MMP_RS02835 and MMP_RS02840 are separated by 23 nucleotides
MMP_RS02825: MMP_RS02825 - mechanosensitive ion channel family protein, at 539,194 to 540,276
_RS02825
MMP_RS02830: MMP_RS02830 - DUF432 domain-containing protein, at 540,277 to 540,915
_RS02830
MMP_RS02835: MMP_RS02835 - CGGC domain-containing protein, at 541,064 to 541,477
_RS02835
MMP_RS02840: MMP_RS02840 - DUF123 domain-containing protein, at 541,501 to 542,571
_RS02840
Position (kb) 540
541 Strain fitness (log2 ratio) -2
-1
0
1
2 at 539.376 kb on - strand, within MMP_RS02825 at 539.405 kb on + strand, within MMP_RS02825 at 539.431 kb on - strand, within MMP_RS02825 at 539.435 kb on + strand, within MMP_RS02825 at 539.438 kb on - strand, within MMP_RS02825 at 539.438 kb on - strand, within MMP_RS02825 at 539.438 kb on - strand, within MMP_RS02825 at 539.438 kb on - strand, within MMP_RS02825 at 539.438 kb on - strand, within MMP_RS02825 at 539.439 kb on + strand, within MMP_RS02825 at 539.445 kb on - strand, within MMP_RS02825 at 539.456 kb on + strand, within MMP_RS02825 at 539.614 kb on + strand, within MMP_RS02825 at 539.724 kb on - strand, within MMP_RS02825 at 539.725 kb on + strand, within MMP_RS02825 at 539.766 kb on - strand, within MMP_RS02825 at 539.930 kb on - strand, within MMP_RS02825 at 539.931 kb on + strand, within MMP_RS02825 at 539.967 kb on + strand, within MMP_RS02825 at 540.013 kb on - strand, within MMP_RS02825 at 540.020 kb on - strand, within MMP_RS02825 at 540.021 kb on + strand, within MMP_RS02825 at 540.393 kb on - strand, within MMP_RS02830 at 540.538 kb on + strand, within MMP_RS02830 at 540.541 kb on - strand, within MMP_RS02830 at 540.542 kb on + strand, within MMP_RS02830 at 540.574 kb on - strand, within MMP_RS02830 at 540.575 kb on + strand, within MMP_RS02830 at 540.575 kb on + strand, within MMP_RS02830 at 540.629 kb on + strand, within MMP_RS02830 at 540.649 kb on - strand, within MMP_RS02830 at 540.678 kb on + strand, within MMP_RS02830 at 540.827 kb on - strand, within MMP_RS02830 at 540.915 kb on - strand at 540.919 kb on + strand at 540.921 kb on - strand at 540.922 kb on + strand at 540.923 kb on - strand at 540.923 kb on - strand at 540.923 kb on - strand at 540.923 kb on - strand at 540.923 kb on - strand at 540.925 kb on - strand at 540.925 kb on - strand at 540.925 kb on - strand at 540.925 kb on - strand at 540.926 kb on + strand at 540.972 kb on + strand at 541.175 kb on + strand, within MMP_RS02835 at 541.222 kb on - strand, within MMP_RS02835 at 541.238 kb on - strand, within MMP_RS02835 at 541.350 kb on - strand, within MMP_RS02835 at 541.454 kb on - strand at 541.456 kb on - strand at 541.456 kb on - strand at 541.457 kb on + strand at 541.460 kb on - strand at 541.519 kb on - strand at 541.654 kb on - strand, within MMP_RS02840 at 541.835 kb on - strand, within MMP_RS02840 at 541.836 kb on + strand, within MMP_RS02840
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 539,376 - MMP_RS02825 0.17 -0.4 539,405 + MMP_RS02825 0.19 +0.6 539,431 - MMP_RS02825 0.22 +0.0 539,435 + MMP_RS02825 0.22 -0.7 539,438 - MMP_RS02825 0.23 -0.3 539,438 - MMP_RS02825 0.23 +0.5 539,438 - MMP_RS02825 0.23 -1.1 539,438 - MMP_RS02825 0.23 +0.4 539,438 - MMP_RS02825 0.23 -0.4 539,439 + MMP_RS02825 0.23 +0.1 539,445 - MMP_RS02825 0.23 -1.2 539,456 + MMP_RS02825 0.24 -0.9 539,614 + MMP_RS02825 0.39 -0.9 539,724 - MMP_RS02825 0.49 -0.6 539,725 + MMP_RS02825 0.49 -1.3 539,766 - MMP_RS02825 0.53 -0.6 539,930 - MMP_RS02825 0.68 +1.1 539,931 + MMP_RS02825 0.68 +1.8 539,967 + MMP_RS02825 0.71 +0.4 540,013 - MMP_RS02825 0.76 -0.3 540,020 - MMP_RS02825 0.76 +0.3 540,021 + MMP_RS02825 0.76 +0.1 540,393 - MMP_RS02830 0.18 +0.3 540,538 + MMP_RS02830 0.41 -0.3 540,541 - MMP_RS02830 0.41 +0.3 540,542 + MMP_RS02830 0.41 +0.3 540,574 - MMP_RS02830 0.46 -1.1 540,575 + MMP_RS02830 0.47 -0.3 540,575 + MMP_RS02830 0.47 -0.4 540,629 + MMP_RS02830 0.55 +0.3 540,649 - MMP_RS02830 0.58 -0.9 540,678 + MMP_RS02830 0.63 -2.2 540,827 - MMP_RS02830 0.86 -0.2 540,915 - +0.0 540,919 + +1.0 540,921 - +0.9 540,922 + -0.2 540,923 - +1.1 540,923 - +0.6 540,923 - -0.5 540,923 - +0.5 540,923 - -0.9 540,925 - -1.3 540,925 - -0.6 540,925 - +0.4 540,925 - -1.5 540,926 + +0.7 540,972 + -0.5 541,175 + MMP_RS02835 0.27 -0.3 541,222 - MMP_RS02835 0.38 -1.4 541,238 - MMP_RS02835 0.42 -0.1 541,350 - MMP_RS02835 0.69 -0.8 541,454 - +1.6 541,456 - +0.2 541,456 - +0.9 541,457 + -0.4 541,460 - +0.8 541,519 - -0.2 541,654 - MMP_RS02840 0.14 +0.2 541,835 - MMP_RS02840 0.31 -1.6 541,836 + MMP_RS02840 0.31 -0.5
Or see this region's nucleotide sequence