Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02685 and MMP_RS02690 are separated by 586 nucleotides MMP_RS02690 and MMP_RS02695 are separated by 43 nucleotides
MMP_RS02685: MMP_RS02685 - molybdate ABC transporter substrate-binding protein, at 510,908 to 511,690
_RS02685
MMP_RS02690: MMP_RS02690 - FmdE family protein, at 512,277 to 512,852
_RS02690
MMP_RS02695: MMP_RS02695 - formylmethanofuran dehydrogenase subunit A, at 512,896 to 514,647
_RS02695
Position (kb) 512
513 Strain fitness (log2 ratio) -2
-1
0
1
2 at 511.403 kb on + strand, within MMP_RS02685 at 511.445 kb on - strand, within MMP_RS02685 at 511.446 kb on + strand, within MMP_RS02685 at 511.584 kb on - strand, within MMP_RS02685 at 511.585 kb on + strand, within MMP_RS02685 at 511.593 kb on + strand, within MMP_RS02685 at 511.596 kb on + strand, within MMP_RS02685 at 511.596 kb on + strand, within MMP_RS02685 at 511.781 kb on - strand at 511.782 kb on + strand at 511.782 kb on + strand at 511.785 kb on - strand at 511.989 kb on - strand at 512.139 kb on - strand at 512.140 kb on + strand at 512.191 kb on + strand at 512.194 kb on - strand at 512.194 kb on - strand at 512.195 kb on + strand at 512.250 kb on - strand at 512.251 kb on + strand at 512.262 kb on - strand at 512.263 kb on + strand at 512.275 kb on + strand at 512.275 kb on + strand at 512.275 kb on + strand at 512.282 kb on - strand at 512.344 kb on - strand, within MMP_RS02690 at 512.400 kb on + strand, within MMP_RS02690 at 512.611 kb on - strand, within MMP_RS02690 at 512.634 kb on - strand, within MMP_RS02690 at 512.675 kb on + strand, within MMP_RS02690 at 512.682 kb on + strand, within MMP_RS02690 at 512.724 kb on - strand, within MMP_RS02690 at 512.749 kb on - strand, within MMP_RS02690 at 512.878 kb on - strand at 512.911 kb on + strand at 512.912 kb on - strand at 512.939 kb on - strand at 512.939 kb on - strand at 512.940 kb on + strand at 512.960 kb on - strand at 513.018 kb on + strand at 513.042 kb on + strand at 513.139 kb on - strand, within MMP_RS02695 at 513.371 kb on - strand, within MMP_RS02695 at 513.442 kb on + strand, within MMP_RS02695 at 513.445 kb on + strand, within MMP_RS02695 at 513.832 kb on - strand, within MMP_RS02695
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 511,403 + MMP_RS02685 0.63 +1.1 511,445 - MMP_RS02685 0.69 -0.4 511,446 + MMP_RS02685 0.69 +1.1 511,584 - MMP_RS02685 0.86 -0.6 511,585 + MMP_RS02685 0.86 +0.0 511,593 + MMP_RS02685 0.87 -0.2 511,596 + MMP_RS02685 0.88 -0.5 511,596 + MMP_RS02685 0.88 -0.3 511,781 - +0.2 511,782 + +1.8 511,782 + -0.5 511,785 - -0.1 511,989 - -0.3 512,139 - -2.6 512,140 + +0.1 512,191 + -2.2 512,194 - +0.1 512,194 - +2.0 512,195 + -0.2 512,250 - -0.3 512,251 + -1.5 512,262 - +0.8 512,263 + +0.0 512,275 + -1.3 512,275 + -0.1 512,275 + +1.2 512,282 - -0.6 512,344 - MMP_RS02690 0.12 -0.6 512,400 + MMP_RS02690 0.21 -0.7 512,611 - MMP_RS02690 0.58 -2.5 512,634 - MMP_RS02690 0.62 +0.0 512,675 + MMP_RS02690 0.69 +0.2 512,682 + MMP_RS02690 0.70 +0.6 512,724 - MMP_RS02690 0.78 -0.4 512,749 - MMP_RS02690 0.82 -1.5 512,878 - -0.7 512,911 + -0.0 512,912 - -0.9 512,939 - -0.6 512,939 - +0.7 512,940 + +0.8 512,960 - -1.2 513,018 + +0.2 513,042 + +0.5 513,139 - MMP_RS02695 0.14 +0.4 513,371 - MMP_RS02695 0.27 +1.8 513,442 + MMP_RS02695 0.31 +0.4 513,445 + MMP_RS02695 0.31 -0.7 513,832 - MMP_RS02695 0.53 -0.4
Or see this region's nucleotide sequence