Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02430 and MMP_RS02435 are separated by 71 nucleotides MMP_RS02435 and MMP_RS02440 are separated by 345 nucleotides
MMP_RS02430: MMP_RS02430 - hypothetical protein, at 452,313 to 453,959
_RS02430
MMP_RS02435: MMP_RS02435 - tRNA-Ser, at 454,031 to 454,115
_RS02435
MMP_RS02440: MMP_RS02440 - hypothetical protein, at 454,461 to 454,838
_RS02440
Position (kb) 454
455 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 453.079 kb on + strand, within MMP_RS02430 at 453.313 kb on + strand, within MMP_RS02430 at 453.313 kb on + strand, within MMP_RS02430 at 453.451 kb on - strand, within MMP_RS02430 at 453.452 kb on + strand, within MMP_RS02430 at 453.459 kb on + strand, within MMP_RS02430 at 453.459 kb on + strand, within MMP_RS02430 at 453.463 kb on - strand, within MMP_RS02430 at 453.633 kb on + strand, within MMP_RS02430 at 453.670 kb on - strand, within MMP_RS02430 at 453.671 kb on + strand, within MMP_RS02430 at 453.676 kb on - strand, within MMP_RS02430 at 453.677 kb on + strand, within MMP_RS02430 at 453.693 kb on - strand, within MMP_RS02430 at 453.693 kb on - strand, within MMP_RS02430 at 453.722 kb on - strand, within MMP_RS02430 at 453.734 kb on - strand, within MMP_RS02430 at 453.735 kb on + strand, within MMP_RS02430 at 453.745 kb on - strand, within MMP_RS02430 at 453.858 kb on + strand at 453.928 kb on - strand at 453.928 kb on - strand at 453.928 kb on - strand at 453.931 kb on - strand at 453.965 kb on + strand at 453.970 kb on - strand at 453.971 kb on + strand at 454.170 kb on - strand at 454.185 kb on - strand at 454.196 kb on - strand at 454.312 kb on + strand at 454.312 kb on + strand at 454.314 kb on - strand at 454.315 kb on + strand at 454.504 kb on + strand, within MMP_RS02440 at 454.506 kb on + strand, within MMP_RS02440 at 454.506 kb on + strand, within MMP_RS02440 at 454.515 kb on - strand, within MMP_RS02440 at 454.516 kb on + strand, within MMP_RS02440 at 454.573 kb on + strand, within MMP_RS02440 at 454.624 kb on - strand, within MMP_RS02440 at 454.624 kb on - strand, within MMP_RS02440 at 454.841 kb on + strand at 454.862 kb on - strand at 454.944 kb on - strand at 454.944 kb on - strand at 454.969 kb on + strand at 454.988 kb on + strand at 454.989 kb on - strand at 455.021 kb on + strand at 455.039 kb on + strand at 455.039 kb on + strand at 455.060 kb on - strand at 455.061 kb on + strand at 455.061 kb on + strand at 455.099 kb on + strand at 455.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 453,079 + MMP_RS02430 0.47 +2.2 453,313 + MMP_RS02430 0.61 +1.3 453,313 + MMP_RS02430 0.61 +0.1 453,451 - MMP_RS02430 0.69 +0.7 453,452 + MMP_RS02430 0.69 +0.1 453,459 + MMP_RS02430 0.70 -1.0 453,459 + MMP_RS02430 0.70 -0.4 453,463 - MMP_RS02430 0.70 +0.7 453,633 + MMP_RS02430 0.80 -0.2 453,670 - MMP_RS02430 0.82 -0.8 453,671 + MMP_RS02430 0.82 -1.9 453,676 - MMP_RS02430 0.83 +0.1 453,677 + MMP_RS02430 0.83 -1.1 453,693 - MMP_RS02430 0.84 -1.4 453,693 - MMP_RS02430 0.84 -0.3 453,722 - MMP_RS02430 0.86 +0.4 453,734 - MMP_RS02430 0.86 +0.8 453,735 + MMP_RS02430 0.86 -1.3 453,745 - MMP_RS02430 0.87 +0.7 453,858 + +1.9 453,928 - -0.1 453,928 - -1.4 453,928 - -0.6 453,931 - +0.6 453,965 + +0.2 453,970 - -0.7 453,971 + +0.1 454,170 - -0.5 454,185 - +0.7 454,196 - +1.5 454,312 + -1.2 454,312 + -0.1 454,314 - +1.1 454,315 + -0.6 454,504 + MMP_RS02440 0.11 -0.6 454,506 + MMP_RS02440 0.12 -0.6 454,506 + MMP_RS02440 0.12 -0.7 454,515 - MMP_RS02440 0.14 +0.2 454,516 + MMP_RS02440 0.15 +0.7 454,573 + MMP_RS02440 0.30 +0.1 454,624 - MMP_RS02440 0.43 +0.2 454,624 - MMP_RS02440 0.43 -0.7 454,841 + -1.8 454,862 - +0.4 454,944 - +0.6 454,944 - +2.4 454,969 + +5.0 454,988 + +0.1 454,989 - -1.1 455,021 + +0.5 455,039 + -0.5 455,039 + +1.0 455,060 - -0.3 455,061 + +1.4 455,061 + +0.1 455,099 + +1.1 455,109 - +0.2
Or see this region's nucleotide sequence