Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02385 and MMP_RS02390 are separated by 9 nucleotides MMP_RS02390 and MMP_RS02395 are separated by 178 nucleotides MMP_RS02395 and MMP_RS02400 are separated by 41 nucleotides
MMP_RS02385: MMP_RS02385 - ATP-binding protein, at 444,206 to 445,063
_RS02385
MMP_RS02390: MMP_RS02390 - NifB/NifX family molybdenum-iron cluster-binding protein, at 445,073 to 445,432
_RS02390
MMP_RS02395: MMP_RS02395 - hypothetical protein, at 445,611 to 445,883
_RS02395
MMP_RS02400: MMP_RS02400 - site-2 protease family protein, at 445,925 to 446,938
_RS02400
Position (kb) 445
446 Strain fitness (log2 ratio) -1
0
1 at 444.828 kb on + strand, within MMP_RS02385 at 444.964 kb on + strand, within MMP_RS02385 at 444.964 kb on + strand, within MMP_RS02385 at 445.010 kb on + strand at 445.014 kb on + strand at 445.050 kb on + strand at 445.057 kb on - strand at 445.078 kb on - strand at 445.223 kb on + strand, within MMP_RS02390 at 445.236 kb on + strand, within MMP_RS02390 at 445.309 kb on - strand, within MMP_RS02390 at 445.353 kb on + strand, within MMP_RS02390 at 445.355 kb on + strand, within MMP_RS02390 at 445.561 kb on + strand at 445.568 kb on - strand at 445.570 kb on - strand at 445.623 kb on - strand at 445.638 kb on - strand at 445.691 kb on - strand, within MMP_RS02395 at 445.692 kb on + strand, within MMP_RS02395 at 445.692 kb on + strand, within MMP_RS02395 at 445.719 kb on + strand, within MMP_RS02395 at 445.722 kb on - strand, within MMP_RS02395 at 445.734 kb on - strand, within MMP_RS02395 at 445.764 kb on - strand, within MMP_RS02395 at 445.772 kb on - strand, within MMP_RS02395 at 445.773 kb on + strand, within MMP_RS02395 at 445.773 kb on + strand, within MMP_RS02395 at 445.802 kb on + strand, within MMP_RS02395 at 445.809 kb on - strand, within MMP_RS02395 at 445.815 kb on - strand, within MMP_RS02395 at 445.816 kb on + strand, within MMP_RS02395 at 445.839 kb on + strand, within MMP_RS02395 at 445.947 kb on + strand at 445.951 kb on - strand at 445.979 kb on + strand at 445.985 kb on - strand at 446.030 kb on + strand, within MMP_RS02400 at 446.096 kb on - strand, within MMP_RS02400 at 446.106 kb on + strand, within MMP_RS02400 at 446.111 kb on + strand, within MMP_RS02400 at 446.129 kb on + strand, within MMP_RS02400 at 446.129 kb on + strand, within MMP_RS02400 at 446.149 kb on - strand, within MMP_RS02400 at 446.259 kb on + strand, within MMP_RS02400 at 446.272 kb on - strand, within MMP_RS02400 at 446.273 kb on + strand, within MMP_RS02400 at 446.273 kb on + strand, within MMP_RS02400 at 446.276 kb on + strand, within MMP_RS02400 at 446.301 kb on + strand, within MMP_RS02400 at 446.305 kb on - strand, within MMP_RS02400 at 446.305 kb on - strand, within MMP_RS02400 at 446.306 kb on + strand, within MMP_RS02400 at 446.306 kb on + strand, within MMP_RS02400 at 446.351 kb on + strand, within MMP_RS02400 at 446.387 kb on - strand, within MMP_RS02400 at 446.403 kb on + strand, within MMP_RS02400 at 446.411 kb on - strand, within MMP_RS02400 at 446.458 kb on - strand, within MMP_RS02400 at 446.459 kb on + strand, within MMP_RS02400 at 446.460 kb on - strand, within MMP_RS02400 at 446.494 kb on - strand, within MMP_RS02400 at 446.494 kb on - strand, within MMP_RS02400 at 446.553 kb on + strand, within MMP_RS02400 at 446.775 kb on - strand, within MMP_RS02400 at 446.776 kb on + strand, within MMP_RS02400 at 446.824 kb on + strand, within MMP_RS02400 at 446.877 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 444,828 + MMP_RS02385 0.72 -0.2 444,964 + MMP_RS02385 0.88 +0.2 444,964 + MMP_RS02385 0.88 +0.0 445,010 + -0.4 445,014 + +1.2 445,050 + -0.2 445,057 - +0.2 445,078 - +0.1 445,223 + MMP_RS02390 0.42 +0.8 445,236 + MMP_RS02390 0.45 +0.0 445,309 - MMP_RS02390 0.66 -0.3 445,353 + MMP_RS02390 0.78 -1.0 445,355 + MMP_RS02390 0.78 +0.6 445,561 + +0.8 445,568 - -0.4 445,570 - -0.7 445,623 - +0.1 445,638 - -0.2 445,691 - MMP_RS02395 0.29 -0.8 445,692 + MMP_RS02395 0.30 -0.7 445,692 + MMP_RS02395 0.30 +0.9 445,719 + MMP_RS02395 0.40 +0.1 445,722 - MMP_RS02395 0.41 -0.7 445,734 - MMP_RS02395 0.45 -1.1 445,764 - MMP_RS02395 0.56 +0.1 445,772 - MMP_RS02395 0.59 -0.8 445,773 + MMP_RS02395 0.59 -1.0 445,773 + MMP_RS02395 0.59 +0.5 445,802 + MMP_RS02395 0.70 +0.1 445,809 - MMP_RS02395 0.73 +0.7 445,815 - MMP_RS02395 0.75 -1.3 445,816 + MMP_RS02395 0.75 -0.4 445,839 + MMP_RS02395 0.84 -0.3 445,947 + +0.1 445,951 - -1.1 445,979 + +0.8 445,985 - -1.2 446,030 + MMP_RS02400 0.10 -0.7 446,096 - MMP_RS02400 0.17 -0.6 446,106 + MMP_RS02400 0.18 -1.0 446,111 + MMP_RS02400 0.18 -0.3 446,129 + MMP_RS02400 0.20 -0.2 446,129 + MMP_RS02400 0.20 -0.1 446,149 - MMP_RS02400 0.22 +0.5 446,259 + MMP_RS02400 0.33 +0.5 446,272 - MMP_RS02400 0.34 -1.1 446,273 + MMP_RS02400 0.34 -0.5 446,273 + MMP_RS02400 0.34 +0.7 446,276 + MMP_RS02400 0.35 -0.2 446,301 + MMP_RS02400 0.37 -1.5 446,305 - MMP_RS02400 0.37 -0.1 446,305 - MMP_RS02400 0.37 -0.7 446,306 + MMP_RS02400 0.38 -0.3 446,306 + MMP_RS02400 0.38 +0.7 446,351 + MMP_RS02400 0.42 +0.2 446,387 - MMP_RS02400 0.46 -0.4 446,403 + MMP_RS02400 0.47 -0.8 446,411 - MMP_RS02400 0.48 -0.2 446,458 - MMP_RS02400 0.53 -0.3 446,459 + MMP_RS02400 0.53 +0.8 446,460 - MMP_RS02400 0.53 -0.3 446,494 - MMP_RS02400 0.56 +0.0 446,494 - MMP_RS02400 0.56 +0.5 446,553 + MMP_RS02400 0.62 -0.3 446,775 - MMP_RS02400 0.84 +0.1 446,776 + MMP_RS02400 0.84 -0.5 446,824 + MMP_RS02400 0.89 -0.0 446,877 + -0.6
Or see this region's nucleotide sequence