Strain Fitness in Methanococcus maripaludis S2 around MMP_RS02100

Experiment: H2 McCas- cells grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS02095 and MMP_RS02100 are separated by 200 nucleotidesMMP_RS02100 and MMP_RS02105 are separated by 58 nucleotidesMMP_RS02105 and MMP_RS02110 are separated by 15 nucleotides MMP_RS02095: MMP_RS02095 - phosphoribosylamine--glycine ligase, at 389,726 to 391,060 _RS02095 MMP_RS02100: MMP_RS02100 - metallophosphoesterase, at 391,261 to 391,776 _RS02100 MMP_RS02105: MMP_RS02105 - uroporphyrinogen-III synthase, at 391,835 to 392,569 _RS02105 MMP_RS02110: MMP_RS02110 - hypothetical protein, at 392,585 to 392,863 _RS02110 Position (kb) 391 392Strain fitness (log2 ratio) -1 0 1 2 3at 390.945 kb on - strandat 390.945 kb on - strandat 391.107 kb on - strandat 391.148 kb on + strandat 391.174 kb on - strandat 391.177 kb on + strandat 391.179 kb on - strandat 391.179 kb on - strandat 391.220 kb on - strandat 391.220 kb on - strandat 391.282 kb on - strandat 391.375 kb on - strand, within MMP_RS02100at 391.529 kb on - strand, within MMP_RS02100at 391.565 kb on + strand, within MMP_RS02100at 391.729 kb on - strandat 391.731 kb on - strandat 391.738 kb on - strandat 391.743 kb on + strandat 391.762 kb on - strandat 391.789 kb on - strandat 391.789 kb on - strandat 391.801 kb on - strandat 391.822 kb on + strandat 391.852 kb on - strandat 391.881 kb on - strandat 391.952 kb on - strandat 391.959 kb on - strandat 392.032 kb on - strandat 392.074 kb on - strandat 392.169 kb on + strandat 392.433 kb on - strandat 392.616 kb on - strand, within MMP_RS02110at 392.616 kb on - strand, within MMP_RS02110

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin
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390,945 - +1.4
390,945 - -0.7
391,107 - -0.3
391,148 + -1.2
391,174 - +0.4
391,177 + -0.2
391,179 - -0.1
391,179 - -1.5
391,220 - -0.0
391,220 - +1.2
391,282 - +0.3
391,375 - MMP_RS02100 0.22 -0.7
391,529 - MMP_RS02100 0.52 -0.8
391,565 + MMP_RS02100 0.59 -0.1
391,729 - +1.2
391,731 - +0.3
391,738 - +0.7
391,743 + -0.5
391,762 - +0.2
391,789 - +0.0
391,789 - +0.8
391,801 - -0.8
391,822 + +0.0
391,852 - -0.2
391,881 - -0.6
391,952 - +0.5
391,959 - -0.0
392,032 - +1.2
392,074 - -0.2
392,169 + +3.4
392,433 - -1.0
392,616 - MMP_RS02110 0.11 -0.4
392,616 - MMP_RS02110 0.11 +0.8

Or see this region's nucleotide sequence