Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02005 and MMP_RS02010 are separated by 5 nucleotides MMP_RS02010 and MMP_RS02015 are separated by 28 nucleotides
MMP_RS02005: MMP_RS02005 - RNA ligase, at 373,010 to 374,194
_RS02005
MMP_RS02010: MMP_RS02010 - PHP domain-containing protein, at 374,200 to 374,844
_RS02010
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
Position (kb) 374
375 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 373.421 kb on + strand, within MMP_RS02005 at 373.421 kb on + strand, within MMP_RS02005 at 373.426 kb on - strand, within MMP_RS02005 at 373.427 kb on + strand, within MMP_RS02005 at 373.517 kb on - strand, within MMP_RS02005 at 373.535 kb on + strand, within MMP_RS02005 at 373.574 kb on + strand, within MMP_RS02005 at 373.574 kb on + strand, within MMP_RS02005 at 373.575 kb on - strand, within MMP_RS02005 at 373.692 kb on - strand, within MMP_RS02005 at 373.865 kb on - strand, within MMP_RS02005 at 374.061 kb on - strand, within MMP_RS02005 at 374.141 kb on - strand at 374.181 kb on - strand at 374.472 kb on - strand, within MMP_RS02010 at 374.474 kb on - strand, within MMP_RS02010 at 374.475 kb on + strand, within MMP_RS02010 at 374.476 kb on - strand, within MMP_RS02010 at 374.477 kb on + strand, within MMP_RS02010 at 374.485 kb on - strand, within MMP_RS02010 at 374.487 kb on - strand, within MMP_RS02010 at 374.492 kb on + strand, within MMP_RS02010 at 374.532 kb on - strand, within MMP_RS02010 at 374.576 kb on - strand, within MMP_RS02010 at 374.577 kb on + strand, within MMP_RS02010 at 374.599 kb on - strand, within MMP_RS02010 at 374.599 kb on - strand, within MMP_RS02010 at 374.698 kb on - strand, within MMP_RS02010 at 374.869 kb on - strand at 374.875 kb on + strand at 374.902 kb on - strand at 374.914 kb on - strand at 374.918 kb on + strand at 374.921 kb on - strand at 374.943 kb on - strand at 374.963 kb on - strand at 374.965 kb on - strand at 374.982 kb on + strand at 375.019 kb on - strand, within MMP_RS02015 at 375.038 kb on + strand, within MMP_RS02015 at 375.074 kb on - strand, within MMP_RS02015 at 375.077 kb on + strand, within MMP_RS02015 at 375.479 kb on - strand, within MMP_RS02015 at 375.480 kb on + strand, within MMP_RS02015 at 375.518 kb on - strand, within MMP_RS02015 at 375.626 kb on + strand, within MMP_RS02015 at 375.665 kb on - strand, within MMP_RS02015 at 375.667 kb on - strand, within MMP_RS02015 at 375.835 kb on + strand, within MMP_RS02015
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 373,421 + MMP_RS02005 0.35 +0.4 373,421 + MMP_RS02005 0.35 +0.6 373,426 - MMP_RS02005 0.35 +0.9 373,427 + MMP_RS02005 0.35 -0.3 373,517 - MMP_RS02005 0.43 +0.4 373,535 + MMP_RS02005 0.44 +0.6 373,574 + MMP_RS02005 0.48 +0.6 373,574 + MMP_RS02005 0.48 +0.0 373,575 - MMP_RS02005 0.48 -0.3 373,692 - MMP_RS02005 0.58 +0.3 373,865 - MMP_RS02005 0.72 -1.9 374,061 - MMP_RS02005 0.89 +1.4 374,141 - +0.4 374,181 - -0.3 374,472 - MMP_RS02010 0.42 -1.4 374,474 - MMP_RS02010 0.42 -0.9 374,475 + MMP_RS02010 0.43 +0.1 374,476 - MMP_RS02010 0.43 -0.8 374,477 + MMP_RS02010 0.43 -0.9 374,485 - MMP_RS02010 0.44 -1.4 374,487 - MMP_RS02010 0.44 -1.1 374,492 + MMP_RS02010 0.45 -2.6 374,532 - MMP_RS02010 0.51 -1.1 374,576 - MMP_RS02010 0.58 -2.1 374,577 + MMP_RS02010 0.58 -2.4 374,599 - MMP_RS02010 0.62 -0.8 374,599 - MMP_RS02010 0.62 -3.1 374,698 - MMP_RS02010 0.77 -2.0 374,869 - -0.4 374,875 + -1.1 374,902 - -0.6 374,914 - -1.1 374,918 + +0.1 374,921 - -0.2 374,943 - +1.5 374,963 - -0.6 374,965 - +0.1 374,982 + +1.5 375,019 - MMP_RS02015 0.11 +0.2 375,038 + MMP_RS02015 0.13 +2.8 375,074 - MMP_RS02015 0.16 +0.7 375,077 + MMP_RS02015 0.16 +0.4 375,479 - MMP_RS02015 0.47 +1.5 375,480 + MMP_RS02015 0.47 -0.5 375,518 - MMP_RS02015 0.50 +0.5 375,626 + MMP_RS02015 0.59 -1.4 375,665 - MMP_RS02015 0.62 -0.2 375,667 - MMP_RS02015 0.62 -0.7 375,835 + MMP_RS02015 0.75 -1.0
Or see this region's nucleotide sequence