Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01860 and MMP_RS01865 are separated by 90 nucleotides MMP_RS01865 and MMP_RS01870 are separated by 126 nucleotides MMP_RS01870 and MMP_RS01875 are separated by 140 nucleotides MMP_RS01875 and MMP_RS01880 are separated by 1 nucleotides
MMP_RS01860: MMP_RS01860 - hypothetical protein, at 343,814 to 344,128
_RS01860
MMP_RS01865: MMP_RS01865 - GPR1/FUN34/YaaH family transporter, at 344,219 to 344,812
_RS01865
MMP_RS01870: MMP_RS01870 - acyl-CoA dehydratase activase, at 344,939 to 345,679
_RS01870
MMP_RS01875: MMP_RS01875 - N-acetyltransferase, at 345,820 to 346,410
_RS01875
MMP_RS01880: MMP_RS01880 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 346,412 to 347,515
_RS01880
Position (kb) 344
345
346 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 343.992 kb on + strand, within MMP_RS01860 at 344.004 kb on - strand, within MMP_RS01860 at 344.023 kb on - strand, within MMP_RS01860 at 344.096 kb on - strand, within MMP_RS01860 at 344.099 kb on + strand at 344.103 kb on + strand at 344.115 kb on + strand at 344.128 kb on - strand at 344.182 kb on - strand at 344.182 kb on - strand at 344.182 kb on - strand at 344.183 kb on + strand at 344.184 kb on - strand at 344.184 kb on - strand at 344.185 kb on + strand at 344.186 kb on - strand at 344.187 kb on + strand at 344.187 kb on + strand at 344.388 kb on + strand, within MMP_RS01865 at 344.790 kb on + strand at 344.819 kb on + strand at 344.856 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.859 kb on + strand at 344.859 kb on + strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.861 kb on + strand at 344.861 kb on + strand at 344.873 kb on + strand at 344.884 kb on - strand at 344.910 kb on - strand at 344.913 kb on + strand at 344.924 kb on + strand at 345.036 kb on + strand, within MMP_RS01870 at 345.078 kb on + strand, within MMP_RS01870 at 345.139 kb on + strand, within MMP_RS01870 at 345.140 kb on - strand, within MMP_RS01870 at 345.141 kb on + strand, within MMP_RS01870 at 345.289 kb on - strand, within MMP_RS01870 at 345.372 kb on + strand, within MMP_RS01870 at 345.372 kb on + strand, within MMP_RS01870 at 345.485 kb on - strand, within MMP_RS01870 at 345.560 kb on - strand, within MMP_RS01870 at 345.605 kb on + strand, within MMP_RS01870 at 345.614 kb on - strand at 345.671 kb on - strand at 345.729 kb on - strand at 345.735 kb on - strand at 345.739 kb on - strand at 345.739 kb on - strand at 345.740 kb on + strand at 345.743 kb on - strand at 345.756 kb on + strand at 345.757 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.775 kb on + strand at 345.775 kb on + strand at 345.783 kb on - strand at 345.789 kb on + strand at 345.816 kb on + strand at 345.826 kb on + strand at 345.829 kb on - strand at 345.883 kb on + strand, within MMP_RS01875 at 345.958 kb on - strand, within MMP_RS01875 at 345.958 kb on - strand, within MMP_RS01875 at 345.959 kb on + strand, within MMP_RS01875 at 345.959 kb on + strand, within MMP_RS01875 at 345.959 kb on + strand, within MMP_RS01875 at 345.960 kb on - strand, within MMP_RS01875 at 345.961 kb on + strand, within MMP_RS01875 at 345.962 kb on - strand, within MMP_RS01875 at 345.962 kb on - strand, within MMP_RS01875 at 345.962 kb on - strand, within MMP_RS01875 at 345.963 kb on + strand, within MMP_RS01875 at 345.963 kb on + strand, within MMP_RS01875 at 345.967 kb on + strand, within MMP_RS01875 at 345.967 kb on + strand, within MMP_RS01875 at 346.016 kb on - strand, within MMP_RS01875 at 346.017 kb on + strand, within MMP_RS01875 at 346.018 kb on - strand, within MMP_RS01875 at 346.019 kb on + strand, within MMP_RS01875 at 346.019 kb on + strand, within MMP_RS01875 at 346.035 kb on + strand, within MMP_RS01875 at 346.067 kb on - strand, within MMP_RS01875 at 346.082 kb on - strand, within MMP_RS01875 at 346.104 kb on - strand, within MMP_RS01875 at 346.167 kb on + strand, within MMP_RS01875 at 346.247 kb on - strand, within MMP_RS01875 at 346.271 kb on - strand, within MMP_RS01875 at 346.272 kb on + strand, within MMP_RS01875 at 346.287 kb on - strand, within MMP_RS01875 at 346.287 kb on - strand, within MMP_RS01875 at 346.288 kb on + strand, within MMP_RS01875 at 346.288 kb on + strand, within MMP_RS01875 at 346.343 kb on + strand, within MMP_RS01875 at 346.350 kb on - strand, within MMP_RS01875 at 346.350 kb on - strand, within MMP_RS01875 at 346.353 kb on - strand at 346.365 kb on - strand at 346.366 kb on + strand at 346.366 kb on + strand at 346.366 kb on + strand at 346.367 kb on - strand at 346.367 kb on - strand at 346.408 kb on - strand at 346.411 kb on - strand at 346.412 kb on + strand at 346.421 kb on + strand at 346.437 kb on - strand at 346.438 kb on + strand at 346.462 kb on + strand at 346.545 kb on - strand, within MMP_RS01880 at 346.645 kb on - strand, within MMP_RS01880 at 346.675 kb on + strand, within MMP_RS01880
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 343,992 + MMP_RS01860 0.57 +0.0 344,004 - MMP_RS01860 0.60 -0.7 344,023 - MMP_RS01860 0.66 -0.8 344,096 - MMP_RS01860 0.90 -1.9 344,099 + +0.8 344,103 + +0.8 344,115 + +0.8 344,128 - -1.3 344,182 - -1.4 344,182 - -0.1 344,182 - +0.6 344,183 + +0.4 344,184 - -1.1 344,184 - -0.2 344,185 + +0.4 344,186 - +1.1 344,187 + +0.3 344,187 + +0.9 344,388 + MMP_RS01865 0.28 -0.4 344,790 + -2.1 344,819 + -0.1 344,856 - -0.5 344,858 - +0.8 344,858 - +0.5 344,858 - +0.3 344,858 - -0.8 344,859 + +1.7 344,859 + +0.8 344,860 - +0.6 344,860 - -0.4 344,860 - +1.7 344,860 - +1.7 344,861 + +1.8 344,861 + -0.6 344,873 + -0.2 344,884 - -0.6 344,910 - -0.5 344,913 + -0.4 344,924 + +0.1 345,036 + MMP_RS01870 0.13 -0.4 345,078 + MMP_RS01870 0.19 -0.2 345,139 + MMP_RS01870 0.27 -0.4 345,140 - MMP_RS01870 0.27 -1.3 345,141 + MMP_RS01870 0.27 -0.4 345,289 - MMP_RS01870 0.47 -0.3 345,372 + MMP_RS01870 0.58 -0.6 345,372 + MMP_RS01870 0.58 -0.7 345,485 - MMP_RS01870 0.74 -0.2 345,560 - MMP_RS01870 0.84 +0.2 345,605 + MMP_RS01870 0.90 -0.3 345,614 - +0.7 345,671 - -0.3 345,729 - -2.2 345,735 - +0.1 345,739 - +0.2 345,739 - -0.6 345,740 + -0.7 345,743 - +0.0 345,756 + -1.9 345,757 - -1.5 345,774 - +2.0 345,774 - +0.2 345,774 - +0.3 345,775 + +0.4 345,775 + +1.4 345,783 - -0.5 345,789 + -0.9 345,816 + +2.4 345,826 + -0.8 345,829 - +0.4 345,883 + MMP_RS01875 0.11 +1.2 345,958 - MMP_RS01875 0.23 +1.8 345,958 - MMP_RS01875 0.23 -1.3 345,959 + MMP_RS01875 0.24 -0.4 345,959 + MMP_RS01875 0.24 +0.6 345,959 + MMP_RS01875 0.24 +1.3 345,960 - MMP_RS01875 0.24 +0.8 345,961 + MMP_RS01875 0.24 +4.4 345,962 - MMP_RS01875 0.24 +0.2 345,962 - MMP_RS01875 0.24 -0.5 345,962 - MMP_RS01875 0.24 -0.3 345,963 + MMP_RS01875 0.24 +1.1 345,963 + MMP_RS01875 0.24 -0.3 345,967 + MMP_RS01875 0.25 -0.7 345,967 + MMP_RS01875 0.25 +1.8 346,016 - MMP_RS01875 0.33 -0.4 346,017 + MMP_RS01875 0.33 +3.4 346,018 - MMP_RS01875 0.34 +2.0 346,019 + MMP_RS01875 0.34 +1.2 346,019 + MMP_RS01875 0.34 +2.4 346,035 + MMP_RS01875 0.36 +0.4 346,067 - MMP_RS01875 0.42 +1.4 346,082 - MMP_RS01875 0.44 +1.4 346,104 - MMP_RS01875 0.48 +0.1 346,167 + MMP_RS01875 0.59 +0.6 346,247 - MMP_RS01875 0.72 +0.6 346,271 - MMP_RS01875 0.76 -1.8 346,272 + MMP_RS01875 0.76 -0.4 346,287 - MMP_RS01875 0.79 +0.4 346,287 - MMP_RS01875 0.79 +0.6 346,288 + MMP_RS01875 0.79 +0.2 346,288 + MMP_RS01875 0.79 +2.7 346,343 + MMP_RS01875 0.88 -1.0 346,350 - MMP_RS01875 0.90 +1.4 346,350 - MMP_RS01875 0.90 -1.1 346,353 - +1.0 346,365 - -1.5 346,366 + +0.9 346,366 + +0.1 346,366 + -0.1 346,367 - +0.8 346,367 - +0.4 346,408 - +0.4 346,411 - +1.8 346,412 + +1.1 346,421 + +1.5 346,437 - -0.7 346,438 + +1.4 346,462 + +1.4 346,545 - MMP_RS01880 0.12 +1.4 346,645 - MMP_RS01880 0.21 +1.8 346,675 + MMP_RS01880 0.24 -0.9
Or see this region's nucleotide sequence