Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01660 and MMP_RS01665 are separated by 54 nucleotides MMP_RS01665 and MMP_RS01670 are separated by 20 nucleotides MMP_RS01670 and MMP_RS01675 are separated by 8 nucleotides MMP_RS01675 and MMP_RS01680 are separated by 314 nucleotides
MMP_RS01660: MMP_RS01660 - hypothetical protein, at 306,663 to 307,247
_RS01660
MMP_RS01665: MMP_RS01665 - DUF2111 domain-containing protein, at 307,302 to 307,685
_RS01665
MMP_RS01670: MMP_RS01670 - methanogenesis marker 5 protein, at 307,706 to 308,146
_RS01670
MMP_RS01675: MMP_RS01675 - hypothetical protein, at 308,155 to 308,610
_RS01675
MMP_RS01680: MMP_RS01680 - phenylacetate--CoA ligase, at 308,925 to 310,169
_RS01680
Position (kb) 308
309 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 307.249 kb on - strand at 307.263 kb on + strand at 307.272 kb on - strand at 307.272 kb on - strand at 307.273 kb on + strand at 307.273 kb on + strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.277 kb on + strand at 307.277 kb on + strand at 307.277 kb on + strand at 307.288 kb on - strand at 307.288 kb on - strand at 307.289 kb on + strand at 307.337 kb on + strand at 307.351 kb on + strand, within MMP_RS01665 at 307.355 kb on - strand, within MMP_RS01665 at 307.363 kb on - strand, within MMP_RS01665 at 307.363 kb on - strand, within MMP_RS01665 at 307.450 kb on + strand, within MMP_RS01665 at 307.609 kb on - strand, within MMP_RS01665 at 307.684 kb on + strand at 308.172 kb on - strand at 308.340 kb on + strand, within MMP_RS01675 at 308.592 kb on + strand at 308.592 kb on + strand at 308.595 kb on + strand at 308.612 kb on - strand at 308.613 kb on + strand at 308.613 kb on + strand at 308.614 kb on - strand at 308.615 kb on + strand at 308.671 kb on - strand at 308.671 kb on - strand at 308.672 kb on + strand at 308.674 kb on + strand at 308.676 kb on + strand at 308.677 kb on - strand at 308.690 kb on - strand at 308.708 kb on - strand at 308.717 kb on + strand at 308.725 kb on - strand at 308.726 kb on + strand at 308.726 kb on + strand at 308.727 kb on - strand at 308.854 kb on - strand at 309.019 kb on - strand at 309.020 kb on + strand at 309.036 kb on - strand at 309.092 kb on - strand, within MMP_RS01680 at 309.097 kb on + strand, within MMP_RS01680 at 309.110 kb on - strand, within MMP_RS01680 at 309.276 kb on + strand, within MMP_RS01680 at 309.344 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.490 kb on + strand, within MMP_RS01680 at 309.509 kb on - strand, within MMP_RS01680
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 307,249 - +0.0 307,263 + +0.1 307,272 - -1.0 307,272 - +0.6 307,273 + +0.5 307,273 + +1.2 307,276 - +0.5 307,276 - -0.6 307,276 - -0.5 307,276 - +5.4 307,276 - +0.5 307,276 - +0.6 307,277 + +0.7 307,277 + -0.0 307,277 + +0.6 307,288 - +1.1 307,288 - +1.5 307,289 + +0.8 307,337 + +1.1 307,351 + MMP_RS01665 0.13 +1.5 307,355 - MMP_RS01665 0.14 +1.3 307,363 - MMP_RS01665 0.16 +0.9 307,363 - MMP_RS01665 0.16 +0.8 307,450 + MMP_RS01665 0.39 +0.7 307,609 - MMP_RS01665 0.80 +1.8 307,684 + +0.6 308,172 - -0.5 308,340 + MMP_RS01675 0.41 -0.6 308,592 + +1.8 308,592 + -0.3 308,595 + -0.1 308,612 - +0.3 308,613 + -0.8 308,613 + -1.1 308,614 - -0.9 308,615 + -0.3 308,671 - -0.4 308,671 - -0.4 308,672 + +1.2 308,674 + +0.5 308,676 + +0.6 308,677 - +0.5 308,690 - +5.9 308,708 - -1.4 308,717 + -0.7 308,725 - +2.4 308,726 + -0.4 308,726 + -0.3 308,727 - -0.8 308,854 - +0.4 309,019 - +0.9 309,020 + -0.4 309,036 - +0.1 309,092 - MMP_RS01680 0.13 -1.1 309,097 + MMP_RS01680 0.14 -0.1 309,110 - MMP_RS01680 0.15 -0.3 309,276 + MMP_RS01680 0.28 +0.7 309,344 - MMP_RS01680 0.34 -0.3 309,489 - MMP_RS01680 0.45 -1.8 309,489 - MMP_RS01680 0.45 -0.9 309,489 - MMP_RS01680 0.45 +0.5 309,490 + MMP_RS01680 0.45 -1.4 309,509 - MMP_RS01680 0.47 -0.9
Or see this region's nucleotide sequence