Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01610 and MMP_RS01615 overlap by 1 nucleotides MMP_RS01615 and MMP_RS01620 are separated by 198 nucleotides MMP_RS01620 and MMP_RS01625 are separated by 14 nucleotides MMP_RS01625 and MMP_RS01630 are separated by 65 nucleotides
MMP_RS01610: MMP_RS01610 - hypothetical protein, at 300,053 to 300,514
_RS01610
MMP_RS01615: MMP_RS01615 - hydrogenase maturation nickel metallochaperone HypA, at 300,514 to 300,894
_RS01615
MMP_RS01620: MMP_RS01620 - hypothetical protein, at 301,093 to 301,452
_RS01620
MMP_RS01625: MMP_RS01625 - rubredoxin, at 301,467 to 301,604
_RS01625
MMP_RS01630: MMP_RS01630 - N-glycosylase/DNA lyase, at 301,670 to 302,428
_RS01630
Position (kb) 301
302 Strain fitness (log2 ratio) -1
0
1
2
3 at 300.178 kb on + strand, within MMP_RS01610 at 300.252 kb on - strand, within MMP_RS01610 at 300.256 kb on - strand, within MMP_RS01610 at 300.256 kb on - strand, within MMP_RS01610 at 300.351 kb on - strand, within MMP_RS01610 at 300.496 kb on - strand at 300.596 kb on - strand at 300.615 kb on + strand at 300.645 kb on + strand at 300.829 kb on + strand at 300.852 kb on - strand at 300.857 kb on - strand at 300.860 kb on - strand at 300.861 kb on + strand at 300.909 kb on - strand at 300.909 kb on - strand at 300.936 kb on - strand at 300.938 kb on - strand at 300.938 kb on - strand at 300.939 kb on + strand at 300.941 kb on + strand at 300.941 kb on + strand at 300.943 kb on - strand at 300.950 kb on - strand at 300.950 kb on - strand at 301.035 kb on - strand at 301.036 kb on + strand at 301.037 kb on - strand at 301.038 kb on + strand at 301.190 kb on - strand, within MMP_RS01620 at 301.191 kb on + strand, within MMP_RS01620 at 301.264 kb on + strand, within MMP_RS01620 at 301.350 kb on - strand, within MMP_RS01620 at 301.350 kb on - strand, within MMP_RS01620 at 301.417 kb on - strand at 301.432 kb on + strand at 301.441 kb on + strand at 301.530 kb on - strand, within MMP_RS01625 at 301.568 kb on + strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.569 kb on - strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.570 kb on + strand, within MMP_RS01625 at 301.582 kb on + strand, within MMP_RS01625 at 301.604 kb on - strand at 301.604 kb on - strand at 301.642 kb on - strand at 301.651 kb on + strand at 301.671 kb on - strand at 301.672 kb on + strand at 301.692 kb on + strand at 301.777 kb on - strand, within MMP_RS01630 at 302.105 kb on - strand, within MMP_RS01630 at 302.233 kb on - strand, within MMP_RS01630 at 302.237 kb on + strand, within MMP_RS01630 at 302.252 kb on - strand, within MMP_RS01630 at 302.338 kb on - strand, within MMP_RS01630 at 302.408 kb on - strand at 302.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 300,178 + MMP_RS01610 0.27 +0.9 300,252 - MMP_RS01610 0.43 -0.5 300,256 - MMP_RS01610 0.44 -1.2 300,256 - MMP_RS01610 0.44 +0.2 300,351 - MMP_RS01610 0.65 +0.4 300,496 - +0.1 300,596 - +0.4 300,615 + +0.3 300,645 + -0.3 300,829 + -1.6 300,852 - +1.0 300,857 - +1.0 300,860 - -0.9 300,861 + +0.4 300,909 - +0.2 300,909 - +0.5 300,936 - +0.7 300,938 - +0.3 300,938 - +0.9 300,939 + +0.3 300,941 + -0.7 300,941 + +1.9 300,943 - -0.1 300,950 - +0.4 300,950 - +1.2 301,035 - -0.0 301,036 + +3.4 301,037 - -1.0 301,038 + +0.3 301,190 - MMP_RS01620 0.27 -1.0 301,191 + MMP_RS01620 0.27 +0.1 301,264 + MMP_RS01620 0.47 -0.3 301,350 - MMP_RS01620 0.71 -0.3 301,350 - MMP_RS01620 0.71 -0.6 301,417 - -0.5 301,432 + +0.0 301,441 + +0.1 301,530 - MMP_RS01625 0.46 +0.4 301,568 + MMP_RS01625 0.73 -0.1 301,569 - MMP_RS01625 0.74 +0.2 301,569 - MMP_RS01625 0.74 +0.0 301,569 - MMP_RS01625 0.74 +0.2 301,569 - MMP_RS01625 0.74 -0.6 301,569 - MMP_RS01625 0.74 -1.1 301,569 - MMP_RS01625 0.74 +0.9 301,569 - MMP_RS01625 0.74 +1.1 301,569 - MMP_RS01625 0.74 -1.2 301,569 - MMP_RS01625 0.74 +0.2 301,569 - MMP_RS01625 0.74 +1.7 301,569 - MMP_RS01625 0.74 +0.8 301,569 - MMP_RS01625 0.74 -0.0 301,569 - MMP_RS01625 0.74 -0.2 301,569 - MMP_RS01625 0.74 -0.8 301,569 - MMP_RS01625 0.74 +0.8 301,569 - MMP_RS01625 0.74 -0.2 301,569 - MMP_RS01625 0.74 +0.2 301,570 + MMP_RS01625 0.75 +1.3 301,570 + MMP_RS01625 0.75 +0.4 301,570 + MMP_RS01625 0.75 -0.6 301,570 + MMP_RS01625 0.75 +0.9 301,570 + MMP_RS01625 0.75 +2.4 301,570 + MMP_RS01625 0.75 +1.1 301,570 + MMP_RS01625 0.75 +0.3 301,570 + MMP_RS01625 0.75 -0.9 301,570 + MMP_RS01625 0.75 +0.2 301,570 + MMP_RS01625 0.75 +1.9 301,570 + MMP_RS01625 0.75 +0.1 301,582 + MMP_RS01625 0.83 -0.4 301,604 - +0.0 301,604 - +0.0 301,642 - +0.4 301,651 + -0.1 301,671 - -0.5 301,672 + +0.5 301,692 + -0.5 301,777 - MMP_RS01630 0.14 +0.8 302,105 - MMP_RS01630 0.57 -0.3 302,233 - MMP_RS01630 0.74 -0.2 302,237 + MMP_RS01630 0.75 -0.6 302,252 - MMP_RS01630 0.77 +0.3 302,338 - MMP_RS01630 0.88 +0.0 302,408 - +1.4 302,447 - +1.1
Or see this region's nucleotide sequence