Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01365 and MMP_RS01370 are separated by 531 nucleotides MMP_RS01370 and MMP_RS01375 are separated by 142 nucleotides
MMP_RS01365: MMP_RS01365 - archaeal proteasome endopeptidase complex subunit alpha, at 252,275 to 253,054
_RS01365
MMP_RS01370: MMP_RS01370 - acetate--CoA ligase family protein, at 253,586 to 255,694
_RS01370
MMP_RS01375: MMP_RS01375 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 255,837 to 257,360
_RS01375
Position (kb) 253
254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 252.681 kb on - strand, within MMP_RS01365 at 252.681 kb on - strand, within MMP_RS01365 at 253.056 kb on - strand at 253.056 kb on - strand at 253.057 kb on + strand at 253.057 kb on + strand at 253.082 kb on + strand at 253.108 kb on - strand at 253.108 kb on - strand at 253.109 kb on + strand at 253.109 kb on + strand at 253.115 kb on - strand at 253.133 kb on - strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.140 kb on + strand at 253.140 kb on + strand at 253.140 kb on + strand at 253.175 kb on - strand at 253.176 kb on + strand at 253.176 kb on + strand at 253.178 kb on + strand at 253.186 kb on + strand at 253.200 kb on - strand at 253.210 kb on - strand at 253.216 kb on - strand at 253.226 kb on - strand at 253.451 kb on + strand at 253.452 kb on - strand at 253.455 kb on + strand at 253.455 kb on + strand at 253.455 kb on + strand at 253.526 kb on - strand at 253.580 kb on + strand at 253.595 kb on - strand at 253.595 kb on - strand at 253.597 kb on - strand at 253.597 kb on - strand at 253.598 kb on + strand at 253.602 kb on + strand at 253.741 kb on - strand at 254.087 kb on - strand, within MMP_RS01370 at 254.088 kb on + strand, within MMP_RS01370 at 254.089 kb on - strand, within MMP_RS01370 at 254.089 kb on - strand, within MMP_RS01370 at 254.143 kb on - strand, within MMP_RS01370 at 254.156 kb on + strand, within MMP_RS01370 at 254.161 kb on - strand, within MMP_RS01370 at 254.162 kb on + strand, within MMP_RS01370 at 254.485 kb on - strand, within MMP_RS01370 at 254.496 kb on - strand, within MMP_RS01370 at 254.562 kb on + strand, within MMP_RS01370 at 254.579 kb on - strand, within MMP_RS01370 at 254.579 kb on - strand, within MMP_RS01370 at 254.601 kb on + strand, within MMP_RS01370 at 254.624 kb on + strand, within MMP_RS01370 at 254.624 kb on + strand, within MMP_RS01370 at 254.850 kb on + strand, within MMP_RS01370 at 254.910 kb on + strand, within MMP_RS01370 at 254.910 kb on + strand, within MMP_RS01370 at 254.994 kb on + strand, within MMP_RS01370 at 255.099 kb on - strand, within MMP_RS01370 at 255.099 kb on - strand, within MMP_RS01370 at 255.099 kb on - strand, within MMP_RS01370 at 255.172 kb on + strand, within MMP_RS01370 at 255.449 kb on - strand, within MMP_RS01370 at 255.475 kb on - strand, within MMP_RS01370 at 255.479 kb on - strand, within MMP_RS01370 at 255.547 kb on + strand at 255.694 kb on - strand at 255.694 kb on - strand at 255.694 kb on - strand at 255.694 kb on - strand at 255.694 kb on - strand at 255.695 kb on + strand at 255.695 kb on + strand at 255.695 kb on + strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.696 kb on - strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.697 kb on + strand at 255.698 kb on - strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.716 kb on + strand at 255.733 kb on - strand at 255.733 kb on - strand at 255.739 kb on - strand at 255.740 kb on + strand at 255.749 kb on - strand at 255.756 kb on + strand at 255.759 kb on - strand at 255.759 kb on - strand at 255.776 kb on + strand at 255.779 kb on + strand at 255.779 kb on + strand at 255.780 kb on - strand at 255.780 kb on - strand at 255.785 kb on + strand at 256.172 kb on + strand, within MMP_RS01375 at 256.200 kb on + strand, within MMP_RS01375 at 256.322 kb on - strand, within MMP_RS01375 at 256.358 kb on - strand, within MMP_RS01375 at 256.402 kb on - strand, within MMP_RS01375 at 256.418 kb on - strand, within MMP_RS01375 at 256.419 kb on + strand, within MMP_RS01375 at 256.450 kb on - strand, within MMP_RS01375 at 256.451 kb on + strand, within MMP_RS01375 at 256.455 kb on + strand, within MMP_RS01375 at 256.455 kb on + strand, within MMP_RS01375 at 256.490 kb on - strand, within MMP_RS01375 at 256.492 kb on - strand, within MMP_RS01375 at 256.493 kb on + strand, within MMP_RS01375 at 256.561 kb on + strand, within MMP_RS01375
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 252,681 - MMP_RS01365 0.52 -0.7 252,681 - MMP_RS01365 0.52 +0.3 253,056 - -0.9 253,056 - +3.3 253,057 + -0.6 253,057 + +1.0 253,082 + -0.4 253,108 - -1.2 253,108 - -0.7 253,109 + +0.6 253,109 + +0.8 253,115 - +0.1 253,133 - +0.5 253,137 - +1.6 253,137 - +1.0 253,137 - -1.9 253,137 - -2.1 253,138 + +0.3 253,138 + -2.2 253,138 + +0.8 253,138 + +0.2 253,138 + +0.1 253,138 + +0.5 253,139 - +1.4 253,139 - +0.2 253,139 - -0.9 253,139 - -1.5 253,139 - +1.0 253,140 + -0.2 253,140 + +0.6 253,140 + -0.4 253,175 - -0.9 253,176 + -0.5 253,176 + +0.2 253,178 + -0.7 253,186 + +0.4 253,200 - -0.8 253,210 - +0.2 253,216 - +0.8 253,226 - +0.7 253,451 + -2.1 253,452 - +1.8 253,455 + -0.5 253,455 + +1.8 253,455 + -0.8 253,526 - +2.3 253,580 + +0.1 253,595 - +0.9 253,595 - -0.7 253,597 - -0.3 253,597 - +0.8 253,598 + +1.1 253,602 + +0.8 253,741 - +0.4 254,087 - MMP_RS01370 0.24 -0.3 254,088 + MMP_RS01370 0.24 -1.4 254,089 - MMP_RS01370 0.24 +1.2 254,089 - MMP_RS01370 0.24 -1.8 254,143 - MMP_RS01370 0.26 -1.0 254,156 + MMP_RS01370 0.27 -0.7 254,161 - MMP_RS01370 0.27 +0.8 254,162 + MMP_RS01370 0.27 -0.7 254,485 - MMP_RS01370 0.43 -1.5 254,496 - MMP_RS01370 0.43 -0.8 254,562 + MMP_RS01370 0.46 +0.1 254,579 - MMP_RS01370 0.47 -1.0 254,579 - MMP_RS01370 0.47 -0.9 254,601 + MMP_RS01370 0.48 -1.5 254,624 + MMP_RS01370 0.49 +1.8 254,624 + MMP_RS01370 0.49 -0.5 254,850 + MMP_RS01370 0.60 +0.1 254,910 + MMP_RS01370 0.63 +0.8 254,910 + MMP_RS01370 0.63 -1.0 254,994 + MMP_RS01370 0.67 -1.2 255,099 - MMP_RS01370 0.72 -1.2 255,099 - MMP_RS01370 0.72 +1.5 255,099 - MMP_RS01370 0.72 +0.4 255,172 + MMP_RS01370 0.75 +0.0 255,449 - MMP_RS01370 0.88 +0.5 255,475 - MMP_RS01370 0.90 +0.2 255,479 - MMP_RS01370 0.90 -1.5 255,547 + +0.3 255,694 - -1.2 255,694 - -2.0 255,694 - +0.3 255,694 - +1.2 255,694 - +0.3 255,695 + +0.7 255,695 + -0.1 255,695 + +0.8 255,696 - -0.8 255,696 - -0.4 255,696 - +1.9 255,696 - -1.0 255,696 - +0.8 255,696 - -0.4 255,696 - +1.1 255,696 - +1.5 255,696 - +1.3 255,697 + -1.5 255,697 + -1.4 255,697 + -0.9 255,697 + -1.8 255,697 + -1.4 255,697 + +1.0 255,697 + -0.2 255,697 + +0.5 255,697 + -0.7 255,697 + -2.3 255,698 - -2.8 255,715 - -0.1 255,715 - +0.4 255,716 + +0.5 255,733 - +0.8 255,733 - -1.7 255,739 - +0.0 255,740 + -0.1 255,749 - +1.3 255,756 + +0.4 255,759 - +0.2 255,759 - -0.2 255,776 + +0.3 255,779 + -0.1 255,779 + -1.3 255,780 - -0.5 255,780 - +0.0 255,785 + -1.1 256,172 + MMP_RS01375 0.22 +0.3 256,200 + MMP_RS01375 0.24 -0.1 256,322 - MMP_RS01375 0.32 -0.6 256,358 - MMP_RS01375 0.34 +0.8 256,402 - MMP_RS01375 0.37 +1.5 256,418 - MMP_RS01375 0.38 -1.1 256,419 + MMP_RS01375 0.38 -0.0 256,450 - MMP_RS01375 0.40 -2.4 256,451 + MMP_RS01375 0.40 +1.0 256,455 + MMP_RS01375 0.41 -0.1 256,455 + MMP_RS01375 0.41 +0.0 256,490 - MMP_RS01375 0.43 +0.7 256,492 - MMP_RS01375 0.43 +0.6 256,493 + MMP_RS01375 0.43 +0.4 256,561 + MMP_RS01375 0.48 +0.2
Or see this region's nucleotide sequence