Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01355 and MMP_RS01360 are separated by 29 nucleotides MMP_RS01360 and MMP_RS01365 are separated by 98 nucleotides MMP_RS01365 and MMP_RS01370 are separated by 531 nucleotides
MMP_RS01355: MMP_RS01355 - 50S ribosomal protein L37Ae, at 251,152 to 251,442
_RS01355
MMP_RS01360: MMP_RS01360 - ribosome assembly factor SBDS, at 251,472 to 252,176
_RS01360
MMP_RS01365: MMP_RS01365 - archaeal proteasome endopeptidase complex subunit alpha, at 252,275 to 253,054
_RS01365
MMP_RS01370: MMP_RS01370 - acetate--CoA ligase family protein, at 253,586 to 255,694
_RS01370
Position (kb) 252
253
254 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 251.491 kb on + strand at 251.495 kb on - strand at 252.199 kb on + strand at 252.389 kb on - strand, within MMP_RS01365 at 252.454 kb on - strand, within MMP_RS01365 at 252.681 kb on - strand, within MMP_RS01365 at 252.681 kb on - strand, within MMP_RS01365 at 253.056 kb on - strand at 253.056 kb on - strand at 253.057 kb on + strand at 253.057 kb on + strand at 253.082 kb on + strand at 253.108 kb on - strand at 253.108 kb on - strand at 253.109 kb on + strand at 253.109 kb on + strand at 253.115 kb on - strand at 253.133 kb on - strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.138 kb on + strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.139 kb on - strand at 253.140 kb on + strand at 253.140 kb on + strand at 253.140 kb on + strand at 253.175 kb on - strand at 253.176 kb on + strand at 253.176 kb on + strand at 253.178 kb on + strand at 253.186 kb on + strand at 253.200 kb on - strand at 253.210 kb on - strand at 253.216 kb on - strand at 253.226 kb on - strand at 253.451 kb on + strand at 253.452 kb on - strand at 253.455 kb on + strand at 253.455 kb on + strand at 253.455 kb on + strand at 253.526 kb on - strand at 253.580 kb on + strand at 253.595 kb on - strand at 253.595 kb on - strand at 253.597 kb on - strand at 253.597 kb on - strand at 253.598 kb on + strand at 253.602 kb on + strand at 253.741 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 251,491 + +1.8 251,495 - +1.0 252,199 + +2.3 252,389 - MMP_RS01365 0.15 -0.7 252,454 - MMP_RS01365 0.23 -0.4 252,681 - MMP_RS01365 0.52 -0.7 252,681 - MMP_RS01365 0.52 +0.3 253,056 - -0.9 253,056 - +3.3 253,057 + -0.6 253,057 + +1.0 253,082 + -0.4 253,108 - -1.2 253,108 - -0.7 253,109 + +0.6 253,109 + +0.8 253,115 - +0.1 253,133 - +0.5 253,137 - +1.6 253,137 - +1.0 253,137 - -1.9 253,137 - -2.1 253,138 + +0.3 253,138 + -2.2 253,138 + +0.8 253,138 + +0.2 253,138 + +0.1 253,138 + +0.5 253,139 - +1.4 253,139 - +0.2 253,139 - -0.9 253,139 - -1.5 253,139 - +1.0 253,140 + -0.2 253,140 + +0.6 253,140 + -0.4 253,175 - -0.9 253,176 + -0.5 253,176 + +0.2 253,178 + -0.7 253,186 + +0.4 253,200 - -0.8 253,210 - +0.2 253,216 - +0.8 253,226 - +0.7 253,451 + -2.1 253,452 - +1.8 253,455 + -0.5 253,455 + +1.8 253,455 + -0.8 253,526 - +2.3 253,580 + +0.1 253,595 - +0.9 253,595 - -0.7 253,597 - -0.3 253,597 - +0.8 253,598 + +1.1 253,602 + +0.8 253,741 - +0.4
Or see this region's nucleotide sequence