Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01145 and MMP_RS01150 are separated by 155 nucleotides MMP_RS01150 and MMP_RS01155 are separated by 15 nucleotides MMP_RS01155 and MMP_RS01160 are separated by 137 nucleotides
MMP_RS01145: MMP_RS01145 - ABC transporter ATP-binding protein, at 214,016 to 215,158
_RS01145
MMP_RS01150: MMP_RS01150 - aldo/keto reductase, at 215,314 to 216,543
_RS01150
MMP_RS01155: MMP_RS01155 - XRE family transcriptional regulator, at 216,559 to 217,113
_RS01155
MMP_RS01160: MMP_RS01160 - DMT family transporter, at 217,251 to 218,150
_RS01160
Position (kb) 215
216
217 Strain fitness (log2 ratio) -1
0
1
2 at 214.408 kb on + strand, within MMP_RS01145 at 214.474 kb on + strand, within MMP_RS01145 at 214.782 kb on - strand, within MMP_RS01145 at 214.782 kb on - strand, within MMP_RS01145 at 214.903 kb on + strand, within MMP_RS01145 at 214.911 kb on - strand, within MMP_RS01145 at 214.911 kb on - strand, within MMP_RS01145 at 214.916 kb on + strand, within MMP_RS01145 at 214.917 kb on - strand, within MMP_RS01145 at 214.965 kb on - strand, within MMP_RS01145 at 214.965 kb on - strand, within MMP_RS01145 at 214.966 kb on + strand, within MMP_RS01145 at 215.062 kb on + strand at 215.217 kb on - strand at 215.218 kb on + strand at 215.218 kb on + strand at 215.218 kb on + strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.220 kb on + strand at 215.220 kb on + strand at 215.220 kb on + strand at 215.222 kb on + strand at 215.237 kb on - strand at 215.309 kb on - strand at 215.313 kb on - strand at 215.313 kb on - strand at 215.314 kb on + strand at 215.467 kb on + strand, within MMP_RS01150 at 215.603 kb on + strand, within MMP_RS01150 at 215.650 kb on - strand, within MMP_RS01150 at 215.835 kb on - strand, within MMP_RS01150 at 216.216 kb on - strand, within MMP_RS01150 at 216.331 kb on + strand, within MMP_RS01150 at 216.486 kb on + strand at 216.510 kb on - strand at 216.572 kb on - strand at 216.572 kb on - strand at 216.572 kb on - strand at 216.575 kb on + strand at 216.640 kb on + strand, within MMP_RS01155 at 216.739 kb on + strand, within MMP_RS01155 at 216.790 kb on - strand, within MMP_RS01155 at 216.824 kb on + strand, within MMP_RS01155 at 216.857 kb on - strand, within MMP_RS01155 at 216.924 kb on - strand, within MMP_RS01155 at 216.955 kb on - strand, within MMP_RS01155 at 217.120 kb on - strand at 217.136 kb on - strand at 217.140 kb on + strand at 217.163 kb on + strand at 217.171 kb on - strand at 217.180 kb on - strand at 217.180 kb on - strand at 217.181 kb on + strand at 217.230 kb on + strand at 217.232 kb on - strand at 217.233 kb on + strand at 217.238 kb on + strand at 217.379 kb on + strand, within MMP_RS01160 at 217.391 kb on + strand, within MMP_RS01160 at 217.408 kb on - strand, within MMP_RS01160 at 217.464 kb on - strand, within MMP_RS01160 at 217.464 kb on - strand, within MMP_RS01160 at 217.504 kb on + strand, within MMP_RS01160
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 214,408 + MMP_RS01145 0.34 -0.9 214,474 + MMP_RS01145 0.40 -0.5 214,782 - MMP_RS01145 0.67 +1.9 214,782 - MMP_RS01145 0.67 +0.6 214,903 + MMP_RS01145 0.78 -0.0 214,911 - MMP_RS01145 0.78 +0.5 214,911 - MMP_RS01145 0.78 +0.9 214,916 + MMP_RS01145 0.79 +1.7 214,917 - MMP_RS01145 0.79 -0.7 214,965 - MMP_RS01145 0.83 -0.0 214,965 - MMP_RS01145 0.83 -0.5 214,966 + MMP_RS01145 0.83 -0.8 215,062 + +0.6 215,217 - -1.1 215,218 + +0.3 215,218 + +0.6 215,218 + +1.2 215,219 - -0.4 215,219 - -0.1 215,219 - -0.7 215,219 - +0.8 215,219 - -0.7 215,219 - -0.1 215,219 - +0.0 215,220 + -0.5 215,220 + +1.4 215,220 + +1.0 215,222 + -0.3 215,237 - +1.3 215,309 - -0.2 215,313 - +0.1 215,313 - +1.1 215,314 + +0.2 215,467 + MMP_RS01150 0.12 -0.9 215,603 + MMP_RS01150 0.23 -0.4 215,650 - MMP_RS01150 0.27 +0.3 215,835 - MMP_RS01150 0.42 -1.1 216,216 - MMP_RS01150 0.73 -0.6 216,331 + MMP_RS01150 0.83 -0.1 216,486 + +0.3 216,510 - -0.2 216,572 - -0.4 216,572 - +0.4 216,572 - -1.6 216,575 + +1.0 216,640 + MMP_RS01155 0.15 +1.0 216,739 + MMP_RS01155 0.32 +1.2 216,790 - MMP_RS01155 0.42 +0.6 216,824 + MMP_RS01155 0.48 -0.6 216,857 - MMP_RS01155 0.54 +0.2 216,924 - MMP_RS01155 0.66 +0.6 216,955 - MMP_RS01155 0.71 +0.6 217,120 - +1.0 217,136 - -1.6 217,140 + -0.3 217,163 + +0.8 217,171 - +0.3 217,180 - +0.0 217,180 - +0.2 217,181 + +0.6 217,230 + +1.7 217,232 - -1.4 217,233 + +0.2 217,238 + -0.8 217,379 + MMP_RS01160 0.14 +0.3 217,391 + MMP_RS01160 0.16 -0.2 217,408 - MMP_RS01160 0.17 +0.1 217,464 - MMP_RS01160 0.24 -0.4 217,464 - MMP_RS01160 0.24 +1.7 217,504 + MMP_RS01160 0.28 -0.4
Or see this region's nucleotide sequence