Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01105 and MMP_RS01110 are separated by 16 nucleotides MMP_RS01110 and MMP_RS01115 are separated by 26 nucleotides MMP_RS01115 and MMP_RS01120 are separated by 789 nucleotides
MMP_RS01105: MMP_RS01105 - hypothetical protein, at 206,848 to 207,624
_RS01105
MMP_RS01110: MMP_RS01110 - FmdE family protein, at 207,641 to 208,225
_RS01110
MMP_RS01115: MMP_RS01115 - molybdopterin molybdotransferase MoeA, at 208,252 to 209,379
_RS01115
MMP_RS01120: MMP_RS01120 - permease, at 210,169 to 211,125
_RS01120
Position (kb)
208
209
210 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 207.365 kb on - strand, within MMP_RS01105 at 207.383 kb on - strand, within MMP_RS01105 at 207.390 kb on - strand, within MMP_RS01105 at 207.390 kb on - strand, within MMP_RS01105 at 207.652 kb on + strand at 207.817 kb on - strand, within MMP_RS01110 at 208.044 kb on - strand, within MMP_RS01110 at 208.045 kb on + strand, within MMP_RS01110 at 208.165 kb on - strand, within MMP_RS01110 at 208.278 kb on - strand at 208.299 kb on - strand at 208.648 kb on + strand, within MMP_RS01115 at 208.648 kb on + strand, within MMP_RS01115 at 208.929 kb on - strand, within MMP_RS01115 at 208.973 kb on + strand, within MMP_RS01115 at 208.988 kb on - strand, within MMP_RS01115 at 209.078 kb on - strand, within MMP_RS01115 at 209.079 kb on + strand, within MMP_RS01115 at 209.079 kb on + strand, within MMP_RS01115 at 209.111 kb on - strand, within MMP_RS01115 at 209.293 kb on + strand at 209.377 kb on - strand at 209.380 kb on - strand at 209.384 kb on + strand at 209.386 kb on - strand at 209.388 kb on - strand at 209.388 kb on - strand at 209.388 kb on - strand at 209.388 kb on - strand at 209.389 kb on + strand at 209.393 kb on + strand at 209.393 kb on + strand at 209.393 kb on + strand at 209.519 kb on - strand at 209.549 kb on - strand at 209.549 kb on - strand at 209.550 kb on + strand at 209.555 kb on - strand at 209.560 kb on + strand at 209.573 kb on - strand at 209.643 kb on - strand at 209.644 kb on + strand at 209.645 kb on - strand at 209.645 kb on - strand at 209.646 kb on + strand at 209.646 kb on + strand at 209.646 kb on + strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.658 kb on - strand at 209.659 kb on + strand at 209.670 kb on - strand at 209.678 kb on - strand at 209.678 kb on - strand at 209.688 kb on + strand at 209.689 kb on - strand at 209.689 kb on - strand at 209.689 kb on - strand at 209.690 kb on + strand at 209.691 kb on - strand at 209.691 kb on - strand at 209.691 kb on - strand at 209.692 kb on + strand at 209.692 kb on + strand at 209.705 kb on + strand at 209.746 kb on + strand at 209.773 kb on - strand at 209.776 kb on - strand at 209.791 kb on + strand at 209.801 kb on - strand at 209.820 kb on + strand at 209.840 kb on - strand at 209.841 kb on + strand at 209.947 kb on - strand at 209.947 kb on - strand at 209.955 kb on - strand at 209.959 kb on - strand at 209.959 kb on - strand at 209.959 kb on - strand at 210.003 kb on + strand at 210.010 kb on - strand at 210.010 kb on - strand at 210.010 kb on - strand at 210.010 kb on - strand at 210.011 kb on + strand at 210.012 kb on - strand at 210.012 kb on - strand at 210.012 kb on - strand at 210.013 kb on + strand at 210.013 kb on + strand at 210.013 kb on + strand at 210.013 kb on + strand at 210.013 kb on + strand at 210.014 kb on - strand at 210.055 kb on - strand at 210.055 kb on - strand at 210.055 kb on - strand at 210.055 kb on - strand at 210.057 kb on - strand at 210.057 kb on - strand at 210.062 kb on - strand at 210.062 kb on - strand at 210.066 kb on - strand at 210.066 kb on - strand at 210.066 kb on - strand at 210.067 kb on + strand at 210.067 kb on + strand at 210.123 kb on - strand at 210.188 kb on + strand at 210.201 kb on - strand at 210.202 kb on + strand at 210.290 kb on - strand, within MMP_RS01120 at 210.309 kb on + strand, within MMP_RS01120 at 210.309 kb on + strand, within MMP_RS01120 at 210.309 kb on + strand, within MMP_RS01120 at 210.322 kb on - strand, within MMP_RS01120 at 210.327 kb on - strand, within MMP_RS01120 at 210.328 kb on + strand, within MMP_RS01120 at 210.334 kb on - strand, within MMP_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 207,365 - MMP_RS01105 0.67 +0.4 207,383 - MMP_RS01105 0.69 +0.7 207,390 - MMP_RS01105 0.70 -0.3 207,390 - MMP_RS01105 0.70 +0.3 207,652 + -0.5 207,817 - MMP_RS01110 0.30 +1.6 208,044 - MMP_RS01110 0.69 -0.7 208,045 + MMP_RS01110 0.69 +0.7 208,165 - MMP_RS01110 0.90 -0.2 208,278 - -1.4 208,299 - +0.8 208,648 + MMP_RS01115 0.35 +0.3 208,648 + MMP_RS01115 0.35 -0.5 208,929 - MMP_RS01115 0.60 -1.3 208,973 + MMP_RS01115 0.64 +0.5 208,988 - MMP_RS01115 0.65 -0.7 209,078 - MMP_RS01115 0.73 -0.3 209,079 + MMP_RS01115 0.73 +0.1 209,079 + MMP_RS01115 0.73 -0.1 209,111 - MMP_RS01115 0.76 -0.5 209,293 + -0.9 209,377 - -0.5 209,380 - +2.3 209,384 + -0.4 209,386 - +0.2 209,388 - -0.1 209,388 - +0.7 209,388 - +0.2 209,388 - -1.3 209,389 + +0.1 209,393 + +1.1 209,393 + +0.6 209,393 + -0.4 209,519 - -0.7 209,549 - -0.8 209,549 - -0.2 209,550 + -0.4 209,555 - -0.7 209,560 + +1.1 209,573 - -1.2 209,643 - -0.0 209,644 + -0.1 209,645 - -0.1 209,645 - -0.0 209,646 + -2.1 209,646 + -0.3 209,646 + -0.1 209,656 - -0.8 209,656 - +0.2 209,658 - -0.3 209,659 + -0.8 209,670 - -0.0 209,678 - +0.4 209,678 - +0.0 209,688 + -0.5 209,689 - -0.2 209,689 - +0.4 209,689 - +0.2 209,690 + -1.2 209,691 - +1.1 209,691 - -0.9 209,691 - -0.2 209,692 + -0.8 209,692 + +0.3 209,705 + -1.5 209,746 + +0.0 209,773 - +0.2 209,776 - -0.5 209,791 + +3.2 209,801 - -0.0 209,820 + +1.2 209,840 - -0.3 209,841 + +0.6 209,947 - -0.1 209,947 - +0.4 209,955 - +0.5 209,959 - -0.7 209,959 - -0.0 209,959 - -0.9 210,003 + +1.3 210,010 - +0.6 210,010 - -0.7 210,010 - -1.3 210,010 - +0.6 210,011 + -0.8 210,012 - +0.7 210,012 - -1.1 210,012 - +0.6 210,013 + -0.1 210,013 + +0.1 210,013 + -1.4 210,013 + -0.7 210,013 + -0.5 210,014 - -0.4 210,055 - +4.1 210,055 - -0.6 210,055 - -0.1 210,055 - +0.5 210,057 - +0.3 210,057 - -1.0 210,062 - +0.8 210,062 - +0.7 210,066 - +2.2 210,066 - -0.1 210,066 - -0.2 210,067 + +0.4 210,067 + +0.4 210,123 - -0.7 210,188 + -1.0 210,201 - +1.4 210,202 + +1.2 210,290 - MMP_RS01120 0.13 +0.8 210,309 + MMP_RS01120 0.15 -1.1 210,309 + MMP_RS01120 0.15 +0.9 210,309 + MMP_RS01120 0.15 +0.0 210,322 - MMP_RS01120 0.16 -1.6 210,327 - MMP_RS01120 0.17 +0.7 210,328 + MMP_RS01120 0.17 +0.2 210,334 - MMP_RS01120 0.17 +0.3
Or see this region's nucleotide sequence