Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01070 and MMP_RS01075 are separated by 84 nucleotides MMP_RS01075 and MMP_RS01080 are separated by 281 nucleotides
MMP_RS01070: MMP_RS01070 - hypothetical protein, at 200,856 to 201,914
_RS01070
MMP_RS01075: MMP_RS01075 - hypothetical protein, at 201,999 to 202,223
_RS01075
MMP_RS01080: MMP_RS01080 - DUF2202 domain-containing protein, at 202,505 to 203,332
_RS01080
Position (kb) 201
202
203 Strain fitness (log2 ratio) -1
0
1
2 at 201.098 kb on - strand, within MMP_RS01070 at 201.159 kb on - strand, within MMP_RS01070 at 201.237 kb on - strand, within MMP_RS01070 at 201.282 kb on + strand, within MMP_RS01070 at 201.334 kb on + strand, within MMP_RS01070 at 201.336 kb on + strand, within MMP_RS01070 at 201.342 kb on - strand, within MMP_RS01070 at 201.379 kb on + strand, within MMP_RS01070 at 201.380 kb on - strand, within MMP_RS01070 at 201.391 kb on - strand, within MMP_RS01070 at 201.392 kb on + strand, within MMP_RS01070 at 201.437 kb on - strand, within MMP_RS01070 at 201.443 kb on - strand, within MMP_RS01070 at 201.461 kb on - strand, within MMP_RS01070 at 201.466 kb on - strand, within MMP_RS01070 at 201.504 kb on + strand, within MMP_RS01070 at 201.559 kb on + strand, within MMP_RS01070 at 201.583 kb on - strand, within MMP_RS01070 at 201.583 kb on - strand, within MMP_RS01070 at 201.583 kb on - strand, within MMP_RS01070 at 201.588 kb on - strand, within MMP_RS01070 at 201.589 kb on + strand, within MMP_RS01070 at 201.601 kb on + strand, within MMP_RS01070 at 201.620 kb on - strand, within MMP_RS01070 at 201.656 kb on - strand, within MMP_RS01070 at 201.657 kb on + strand, within MMP_RS01070 at 201.658 kb on - strand, within MMP_RS01070 at 201.659 kb on + strand, within MMP_RS01070 at 201.659 kb on + strand, within MMP_RS01070 at 201.659 kb on + strand, within MMP_RS01070 at 201.665 kb on + strand, within MMP_RS01070 at 201.795 kb on - strand, within MMP_RS01070 at 201.888 kb on - strand at 201.889 kb on + strand at 201.894 kb on + strand at 201.895 kb on - strand at 201.933 kb on - strand at 201.938 kb on + strand at 202.195 kb on - strand, within MMP_RS01075 at 202.200 kb on + strand, within MMP_RS01075 at 202.204 kb on - strand at 202.384 kb on + strand at 202.394 kb on - strand at 202.428 kb on - strand at 202.428 kb on - strand at 202.464 kb on - strand at 202.465 kb on + strand at 202.466 kb on - strand at 202.466 kb on - strand at 202.466 kb on - strand at 202.467 kb on + strand at 202.488 kb on + strand at 202.488 kb on + strand at 202.491 kb on + strand at 202.494 kb on - strand at 202.495 kb on + strand at 202.554 kb on - strand at 202.760 kb on - strand, within MMP_RS01080 at 202.771 kb on + strand, within MMP_RS01080 at 202.771 kb on + strand, within MMP_RS01080 at 202.800 kb on - strand, within MMP_RS01080 at 202.814 kb on + strand, within MMP_RS01080 at 202.846 kb on + strand, within MMP_RS01080 at 202.852 kb on + strand, within MMP_RS01080 at 203.169 kb on + strand, within MMP_RS01080
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 201,098 - MMP_RS01070 0.23 -0.4 201,159 - MMP_RS01070 0.29 -0.1 201,237 - MMP_RS01070 0.36 -0.7 201,282 + MMP_RS01070 0.40 -0.8 201,334 + MMP_RS01070 0.45 +0.4 201,336 + MMP_RS01070 0.45 -1.6 201,342 - MMP_RS01070 0.46 +0.5 201,379 + MMP_RS01070 0.49 +1.0 201,380 - MMP_RS01070 0.49 +1.2 201,391 - MMP_RS01070 0.51 +0.7 201,392 + MMP_RS01070 0.51 -1.1 201,437 - MMP_RS01070 0.55 -0.2 201,443 - MMP_RS01070 0.55 +0.2 201,461 - MMP_RS01070 0.57 -0.4 201,466 - MMP_RS01070 0.58 +0.4 201,504 + MMP_RS01070 0.61 -0.5 201,559 + MMP_RS01070 0.66 +0.1 201,583 - MMP_RS01070 0.69 -0.9 201,583 - MMP_RS01070 0.69 +1.9 201,583 - MMP_RS01070 0.69 +1.7 201,588 - MMP_RS01070 0.69 -0.1 201,589 + MMP_RS01070 0.69 +0.2 201,601 + MMP_RS01070 0.70 -0.2 201,620 - MMP_RS01070 0.72 +1.7 201,656 - MMP_RS01070 0.76 +0.4 201,657 + MMP_RS01070 0.76 +2.1 201,658 - MMP_RS01070 0.76 +0.6 201,659 + MMP_RS01070 0.76 +0.4 201,659 + MMP_RS01070 0.76 -1.1 201,659 + MMP_RS01070 0.76 +0.3 201,665 + MMP_RS01070 0.76 -0.3 201,795 - MMP_RS01070 0.89 +0.2 201,888 - -0.5 201,889 + -0.3 201,894 + -0.3 201,895 - -0.4 201,933 - +0.4 201,938 + +0.2 202,195 - MMP_RS01075 0.87 -0.9 202,200 + MMP_RS01075 0.89 +0.3 202,204 - -0.6 202,384 + -0.8 202,394 - -1.2 202,428 - -0.0 202,428 - -0.2 202,464 - +0.3 202,465 + +0.3 202,466 - +0.8 202,466 - -1.0 202,466 - +0.2 202,467 + +0.0 202,488 + +0.8 202,488 + +0.4 202,491 + -1.0 202,494 - +0.4 202,495 + -1.1 202,554 - +0.6 202,760 - MMP_RS01080 0.31 -0.1 202,771 + MMP_RS01080 0.32 -0.7 202,771 + MMP_RS01080 0.32 -0.5 202,800 - MMP_RS01080 0.36 -0.6 202,814 + MMP_RS01080 0.37 -0.2 202,846 + MMP_RS01080 0.41 +0.3 202,852 + MMP_RS01080 0.42 +0.4 203,169 + MMP_RS01080 0.80 -0.7
Or see this region's nucleotide sequence