Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00580 and MMP_RS00585 are separated by 46 nucleotides MMP_RS00585 and MMP_RS00590 are separated by 8 nucleotides MMP_RS00590 and MMP_RS00595 are separated by 16 nucleotides MMP_RS00595 and MMP_RS00600 are separated by 40 nucleotides MMP_RS00600 and MMP_RS00605 are separated by 16 nucleotides MMP_RS00605 and MMP_RS00610 are separated by 33 nucleotides MMP_RS00610 and MMP_RS00615 are separated by 20 nucleotides MMP_RS00615 and MMP_RS00620 are separated by 52 nucleotides MMP_RS00620 and MMP_RS00625 are separated by 89 nucleotides MMP_RS00625 and MMP_RS00630 are separated by 102 nucleotides
MMP_RS00580: MMP_RS00580 - tRNA-Thr, at 112,878 to 112,951
_RS00580
MMP_RS00585: MMP_RS00585 - tRNA-Pro, at 112,998 to 113,074
_RS00585
MMP_RS00590: MMP_RS00590 - tRNA-Tyr, at 113,083 to 113,156
_RS00590
MMP_RS00595: MMP_RS00595 - tRNA-Lys, at 113,173 to 113,246
_RS00595
MMP_RS00600: MMP_RS00600 - 5S ribosomal RNA, at 113,287 to 113,401
_RS00600
MMP_RS00605: MMP_RS00605 - tRNA-Asp, at 113,418 to 113,492
_RS00605
MMP_RS00610: MMP_RS00610 - tRNA-Lys, at 113,526 to 113,599
_RS00610
MMP_RS00615: MMP_RS00615 - tRNA-Asp, at 113,620 to 113,694
_RS00615
MMP_RS00620: MMP_RS00620 - 4Fe-4S binding protein, at 113,747 to 114,364
_RS00620
MMP_RS00625: MMP_RS00625 - hypothetical protein, at 114,454 to 115,014
_RS00625
MMP_RS00630: MMP_RS00630 - ECF transporter S component, at 115,117 to 115,677
_RS00630
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 112.751 kb on - strand at 112.752 kb on + strand at 112.777 kb on - strand at 112.798 kb on - strand at 112.798 kb on - strand at 112.799 kb on + strand at 112.799 kb on + strand at 112.809 kb on - strand at 113.770 kb on - strand at 113.806 kb on - strand at 113.886 kb on - strand, within MMP_RS00620 at 113.911 kb on + strand, within MMP_RS00620 at 113.953 kb on + strand, within MMP_RS00620 at 113.986 kb on + strand, within MMP_RS00620 at 114.042 kb on - strand, within MMP_RS00620 at 114.043 kb on + strand, within MMP_RS00620 at 114.055 kb on + strand, within MMP_RS00620 at 114.122 kb on + strand, within MMP_RS00620 at 114.122 kb on + strand, within MMP_RS00620 at 114.142 kb on - strand, within MMP_RS00620 at 114.229 kb on - strand, within MMP_RS00620 at 114.261 kb on - strand, within MMP_RS00620 at 114.304 kb on + strand at 114.346 kb on + strand at 114.402 kb on - strand at 114.403 kb on + strand at 114.405 kb on + strand at 114.406 kb on - strand at 114.407 kb on + strand at 114.409 kb on - strand at 114.486 kb on + strand at 114.493 kb on + strand at 114.503 kb on - strand at 114.520 kb on - strand, within MMP_RS00625 at 114.525 kb on + strand, within MMP_RS00625 at 114.533 kb on - strand, within MMP_RS00625 at 114.534 kb on + strand, within MMP_RS00625 at 114.535 kb on - strand, within MMP_RS00625 at 114.536 kb on + strand, within MMP_RS00625 at 114.808 kb on - strand, within MMP_RS00625 at 114.880 kb on + strand, within MMP_RS00625 at 114.963 kb on + strand at 114.963 kb on + strand at 114.964 kb on - strand at 114.965 kb on + strand at 114.965 kb on + strand at 115.069 kb on + strand at 115.073 kb on + strand at 115.073 kb on + strand at 115.135 kb on - strand at 115.135 kb on - strand at 115.136 kb on + strand at 115.326 kb on - strand, within MMP_RS00630 at 115.344 kb on + strand, within MMP_RS00630
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 112,751 - -0.1 112,752 + +0.4 112,777 - -0.2 112,798 - +3.3 112,798 - +0.4 112,799 + +0.2 112,799 + +1.7 112,809 - +1.4 113,770 - -1.3 113,806 - -0.5 113,886 - MMP_RS00620 0.22 -1.8 113,911 + MMP_RS00620 0.27 -0.4 113,953 + MMP_RS00620 0.33 +0.5 113,986 + MMP_RS00620 0.39 -0.4 114,042 - MMP_RS00620 0.48 +0.2 114,043 + MMP_RS00620 0.48 -0.6 114,055 + MMP_RS00620 0.50 +0.7 114,122 + MMP_RS00620 0.61 +1.3 114,122 + MMP_RS00620 0.61 -0.4 114,142 - MMP_RS00620 0.64 +0.1 114,229 - MMP_RS00620 0.78 -2.0 114,261 - MMP_RS00620 0.83 +0.1 114,304 + +0.1 114,346 + +0.1 114,402 - +0.6 114,403 + +0.7 114,405 + +0.4 114,406 - -0.5 114,407 + -0.2 114,409 - +1.3 114,486 + +0.6 114,493 + -0.6 114,503 - -0.2 114,520 - MMP_RS00625 0.12 -0.6 114,525 + MMP_RS00625 0.13 +0.1 114,533 - MMP_RS00625 0.14 +0.4 114,534 + MMP_RS00625 0.14 -0.1 114,535 - MMP_RS00625 0.14 -0.4 114,536 + MMP_RS00625 0.15 +1.4 114,808 - MMP_RS00625 0.63 +0.6 114,880 + MMP_RS00625 0.76 -0.9 114,963 + +0.7 114,963 + +0.7 114,964 - -0.3 114,965 + -0.2 114,965 + +0.7 115,069 + -0.5 115,073 + +0.4 115,073 + +0.2 115,135 - -0.2 115,135 - -0.2 115,136 + -0.1 115,326 - MMP_RS00630 0.37 +0.1 115,344 + MMP_RS00630 0.40 -0.9
Or see this region's nucleotide sequence