Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00530 and MMP_RS00535 are separated by 21 nucleotides MMP_RS00535 and MMP_RS00540 are separated by 2 nucleotides
MMP_RS00530: MMP_RS00530 - tRNA pseudouridine(54/55) synthase Pus10, at 103,274 to 104,686
_RS00530
MMP_RS00535: MMP_RS00535 - TIGR00375 family protein, at 104,708 to 105,892
_RS00535
MMP_RS00540: MMP_RS00540 - PLP-dependent aminotransferase family protein, at 105,895 to 107,082
_RS00540
Position (kb) 104
105
106 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 103.766 kb on - strand, within MMP_RS00530 at 103.905 kb on - strand, within MMP_RS00530 at 103.906 kb on + strand, within MMP_RS00530 at 103.906 kb on + strand, within MMP_RS00530 at 104.001 kb on - strand, within MMP_RS00530 at 104.006 kb on + strand, within MMP_RS00530 at 104.006 kb on + strand, within MMP_RS00530 at 104.038 kb on - strand, within MMP_RS00530 at 104.446 kb on + strand, within MMP_RS00530 at 104.608 kb on - strand at 104.673 kb on - strand at 104.674 kb on + strand at 104.674 kb on + strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.679 kb on + strand at 104.679 kb on + strand at 104.679 kb on + strand at 104.687 kb on + strand at 104.687 kb on + strand at 104.688 kb on - strand at 104.716 kb on + strand at 104.727 kb on - strand at 104.727 kb on - strand at 104.727 kb on - strand at 104.728 kb on + strand at 104.728 kb on + strand at 104.728 kb on + strand at 104.731 kb on - strand at 104.732 kb on + strand at 104.744 kb on - strand at 104.750 kb on + strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.762 kb on + strand at 104.762 kb on + strand at 104.762 kb on + strand at 104.765 kb on - strand at 104.765 kb on - strand at 104.766 kb on + strand at 104.766 kb on + strand at 104.767 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.783 kb on + strand at 104.793 kb on + strand at 104.947 kb on - strand, within MMP_RS00535 at 104.947 kb on - strand, within MMP_RS00535 at 105.791 kb on - strand at 106.134 kb on - strand, within MMP_RS00540 at 106.192 kb on + strand, within MMP_RS00540 at 106.193 kb on - strand, within MMP_RS00540 at 106.196 kb on + strand, within MMP_RS00540 at 106.300 kb on - strand, within MMP_RS00540 at 106.300 kb on - strand, within MMP_RS00540 at 106.302 kb on - strand, within MMP_RS00540 at 106.706 kb on - strand, within MMP_RS00540 at 106.706 kb on - strand, within MMP_RS00540 at 106.724 kb on + strand, within MMP_RS00540
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 103,766 - MMP_RS00530 0.35 -1.3 103,905 - MMP_RS00530 0.45 -1.6 103,906 + MMP_RS00530 0.45 +1.1 103,906 + MMP_RS00530 0.45 -1.3 104,001 - MMP_RS00530 0.51 -1.6 104,006 + MMP_RS00530 0.52 -1.6 104,006 + MMP_RS00530 0.52 -1.4 104,038 - MMP_RS00530 0.54 +0.5 104,446 + MMP_RS00530 0.83 +2.9 104,608 - -0.4 104,673 - +0.7 104,674 + +0.7 104,674 + +0.1 104,678 - +1.1 104,678 - +1.0 104,678 - -0.9 104,678 - -0.7 104,679 + -0.3 104,679 + -0.5 104,679 + +0.0 104,687 + -1.0 104,687 + -1.2 104,688 - -0.3 104,716 + +0.8 104,727 - +3.6 104,727 - -1.2 104,727 - +2.3 104,728 + +0.3 104,728 + -0.8 104,728 + -0.6 104,731 - +0.6 104,732 + +0.0 104,744 - +0.8 104,750 + -0.2 104,761 - +1.3 104,761 - -0.1 104,761 - -1.0 104,761 - +0.8 104,761 - +1.8 104,762 + +0.8 104,762 + -1.5 104,762 + +0.5 104,765 - +2.7 104,765 - -0.1 104,766 + -1.2 104,766 + -0.6 104,767 - +0.1 104,782 - +0.3 104,782 - +0.3 104,782 - +1.5 104,782 - -0.2 104,782 - -0.3 104,783 + -1.9 104,793 + +0.8 104,947 - MMP_RS00535 0.20 -2.3 104,947 - MMP_RS00535 0.20 +0.0 105,791 - -0.4 106,134 - MMP_RS00540 0.20 +0.4 106,192 + MMP_RS00540 0.25 -0.4 106,193 - MMP_RS00540 0.25 -0.3 106,196 + MMP_RS00540 0.25 -1.5 106,300 - MMP_RS00540 0.34 -0.7 106,300 - MMP_RS00540 0.34 +0.1 106,302 - MMP_RS00540 0.34 +0.7 106,706 - MMP_RS00540 0.68 +0.0 106,706 - MMP_RS00540 0.68 -0.1 106,724 + MMP_RS00540 0.70 +1.5
Or see this region's nucleotide sequence