Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00515 and MMP_RS00520 are separated by 100 nucleotides MMP_RS00520 and MMP_RS00525 are separated by 48 nucleotides MMP_RS00525 and MMP_RS00530 are separated by 85 nucleotides MMP_RS00530 and MMP_RS00535 are separated by 21 nucleotides
MMP_RS00515: MMP_RS00515 - DUF655 domain-containing protein, at 101,838 to 102,422
_RS00515
MMP_RS00520: MMP_RS00520 - RNA polymerase Rpb4 family protein, at 102,523 to 102,846
_RS00520
MMP_RS00525: MMP_RS00525 - 50S ribosomal protein L21e, at 102,895 to 103,188
_RS00525
MMP_RS00530: MMP_RS00530 - tRNA pseudouridine(54/55) synthase Pus10, at 103,274 to 104,686
_RS00530
MMP_RS00535: MMP_RS00535 - TIGR00375 family protein, at 104,708 to 105,892
_RS00535
Position (kb) 103
104
105 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 102.335 kb on - strand, within MMP_RS00515 at 102.373 kb on - strand at 102.379 kb on - strand at 102.454 kb on + strand at 102.460 kb on + strand at 102.463 kb on - strand at 102.463 kb on - strand at 102.479 kb on - strand at 102.541 kb on - strand at 102.622 kb on - strand, within MMP_RS00520 at 102.706 kb on - strand, within MMP_RS00520 at 102.758 kb on + strand, within MMP_RS00520 at 103.258 kb on - strand at 103.296 kb on - strand at 103.393 kb on - strand at 103.446 kb on - strand, within MMP_RS00530 at 103.475 kb on + strand, within MMP_RS00530 at 103.476 kb on - strand, within MMP_RS00530 at 103.766 kb on - strand, within MMP_RS00530 at 103.905 kb on - strand, within MMP_RS00530 at 103.906 kb on + strand, within MMP_RS00530 at 103.906 kb on + strand, within MMP_RS00530 at 104.001 kb on - strand, within MMP_RS00530 at 104.006 kb on + strand, within MMP_RS00530 at 104.006 kb on + strand, within MMP_RS00530 at 104.038 kb on - strand, within MMP_RS00530 at 104.446 kb on + strand, within MMP_RS00530 at 104.608 kb on - strand at 104.673 kb on - strand at 104.674 kb on + strand at 104.674 kb on + strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.679 kb on + strand at 104.679 kb on + strand at 104.679 kb on + strand at 104.687 kb on + strand at 104.687 kb on + strand at 104.688 kb on - strand at 104.716 kb on + strand at 104.727 kb on - strand at 104.727 kb on - strand at 104.727 kb on - strand at 104.728 kb on + strand at 104.728 kb on + strand at 104.728 kb on + strand at 104.731 kb on - strand at 104.732 kb on + strand at 104.744 kb on - strand at 104.750 kb on + strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.761 kb on - strand at 104.762 kb on + strand at 104.762 kb on + strand at 104.762 kb on + strand at 104.765 kb on - strand at 104.765 kb on - strand at 104.766 kb on + strand at 104.766 kb on + strand at 104.767 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.782 kb on - strand at 104.783 kb on + strand at 104.793 kb on + strand at 104.947 kb on - strand, within MMP_RS00535 at 104.947 kb on - strand, within MMP_RS00535
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 102,335 - MMP_RS00515 0.85 -0.2 102,373 - +0.3 102,379 - +1.3 102,454 + +0.5 102,460 + -0.8 102,463 - +2.3 102,463 - +0.3 102,479 - +1.7 102,541 - +1.7 102,622 - MMP_RS00520 0.31 +1.3 102,706 - MMP_RS00520 0.56 +1.7 102,758 + MMP_RS00520 0.73 -0.4 103,258 - +2.3 103,296 - -2.0 103,393 - -0.1 103,446 - MMP_RS00530 0.12 -1.4 103,475 + MMP_RS00530 0.14 -0.2 103,476 - MMP_RS00530 0.14 +0.0 103,766 - MMP_RS00530 0.35 -1.3 103,905 - MMP_RS00530 0.45 -1.6 103,906 + MMP_RS00530 0.45 +1.1 103,906 + MMP_RS00530 0.45 -1.3 104,001 - MMP_RS00530 0.51 -1.6 104,006 + MMP_RS00530 0.52 -1.6 104,006 + MMP_RS00530 0.52 -1.4 104,038 - MMP_RS00530 0.54 +0.5 104,446 + MMP_RS00530 0.83 +2.9 104,608 - -0.4 104,673 - +0.7 104,674 + +0.7 104,674 + +0.1 104,678 - +1.1 104,678 - +1.0 104,678 - -0.9 104,678 - -0.7 104,679 + -0.3 104,679 + -0.5 104,679 + +0.0 104,687 + -1.0 104,687 + -1.2 104,688 - -0.3 104,716 + +0.8 104,727 - +3.6 104,727 - -1.2 104,727 - +2.3 104,728 + +0.3 104,728 + -0.8 104,728 + -0.6 104,731 - +0.6 104,732 + +0.0 104,744 - +0.8 104,750 + -0.2 104,761 - +1.3 104,761 - -0.1 104,761 - -1.0 104,761 - +0.8 104,761 - +1.8 104,762 + +0.8 104,762 + -1.5 104,762 + +0.5 104,765 - +2.7 104,765 - -0.1 104,766 + -1.2 104,766 + -0.6 104,767 - +0.1 104,782 - +0.3 104,782 - +0.3 104,782 - +1.5 104,782 - -0.2 104,782 - -0.3 104,783 + -1.9 104,793 + +0.8 104,947 - MMP_RS00535 0.20 -2.3 104,947 - MMP_RS00535 0.20 +0.0
Or see this region's nucleotide sequence