Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00375 and MMP_RS00380 are separated by 103 nucleotides MMP_RS00380 and MMP_RS00385 are separated by 201 nucleotides MMP_RS00385 and MMP_RS00390 are separated by 28 nucleotides
MMP_RS00375: MMP_RS00375 - ammonium transporter, at 77,116 to 78,342
_RS00375
MMP_RS00380: MMP_RS00380 - P-II family nitrogen regulator, at 78,446 to 78,784
_RS00380
MMP_RS00385: MMP_RS00385 - P-II family nitrogen regulator, at 78,986 to 79,324
_RS00385
MMP_RS00390: MMP_RS00390 - ammonium transporter, at 79,353 to 80,588
_RS00390
Position (kb) 78
79
80 Strain fitness (log2 ratio) -1
0
1
2
3 at 78.068 kb on - strand, within MMP_RS00375 at 78.165 kb on + strand, within MMP_RS00375 at 78.174 kb on + strand, within MMP_RS00375 at 78.273 kb on - strand at 78.372 kb on + strand at 78.375 kb on - strand at 78.376 kb on + strand at 78.378 kb on - strand at 78.378 kb on - strand at 78.378 kb on - strand at 78.379 kb on + strand at 78.381 kb on - strand at 78.381 kb on - strand at 78.381 kb on - strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.384 kb on + strand at 78.617 kb on + strand, within MMP_RS00380 at 78.641 kb on + strand, within MMP_RS00380 at 78.663 kb on - strand, within MMP_RS00380 at 78.723 kb on - strand, within MMP_RS00380 at 78.745 kb on - strand, within MMP_RS00380 at 78.746 kb on + strand, within MMP_RS00380 at 78.782 kb on - strand at 78.783 kb on + strand at 78.833 kb on - strand at 78.847 kb on - strand at 78.847 kb on - strand at 78.848 kb on + strand at 78.848 kb on + strand at 78.972 kb on - strand at 79.213 kb on + strand, within MMP_RS00385 at 79.229 kb on - strand, within MMP_RS00385 at 79.231 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.323 kb on + strand at 79.323 kb on + strand at 79.325 kb on - strand at 79.325 kb on - strand at 79.348 kb on - strand at 79.348 kb on - strand at 79.466 kb on + strand at 79.607 kb on + strand, within MMP_RS00390 at 79.919 kb on + strand, within MMP_RS00390 at 80.013 kb on - strand, within MMP_RS00390 at 80.013 kb on - strand, within MMP_RS00390 at 80.014 kb on + strand, within MMP_RS00390 at 80.072 kb on - strand, within MMP_RS00390 at 80.086 kb on - strand, within MMP_RS00390 at 80.158 kb on - strand, within MMP_RS00390 at 80.158 kb on - strand, within MMP_RS00390 at 80.158 kb on - strand, within MMP_RS00390 at 80.161 kb on + strand, within MMP_RS00390 at 80.161 kb on + strand, within MMP_RS00390 at 80.161 kb on + strand, within MMP_RS00390 at 80.240 kb on + strand, within MMP_RS00390 at 80.279 kb on - strand, within MMP_RS00390 at 80.311 kb on - strand, within MMP_RS00390 at 80.312 kb on + strand, within MMP_RS00390
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 78,068 - MMP_RS00375 0.78 +1.2 78,165 + MMP_RS00375 0.85 -0.2 78,174 + MMP_RS00375 0.86 +1.8 78,273 - -0.9 78,372 + -1.1 78,375 - +0.4 78,376 + -0.0 78,378 - -0.5 78,378 - -0.6 78,378 - +0.1 78,379 + +0.4 78,381 - -1.3 78,381 - +2.3 78,381 - -1.0 78,382 + +0.2 78,382 + -0.1 78,382 + -0.5 78,382 + +2.3 78,384 + -0.6 78,617 + MMP_RS00380 0.50 +1.7 78,641 + MMP_RS00380 0.58 -1.2 78,663 - MMP_RS00380 0.64 +1.0 78,723 - MMP_RS00380 0.82 -0.6 78,745 - MMP_RS00380 0.88 +0.1 78,746 + MMP_RS00380 0.88 -0.3 78,782 - +1.1 78,783 + +2.8 78,833 - +0.4 78,847 - -0.0 78,847 - -0.9 78,848 + +0.3 78,848 + -0.1 78,972 - +0.6 79,213 + MMP_RS00385 0.67 -0.1 79,229 - MMP_RS00385 0.72 +0.2 79,231 - MMP_RS00385 0.72 -0.8 79,281 - MMP_RS00385 0.87 -1.2 79,281 - MMP_RS00385 0.87 -0.3 79,281 - MMP_RS00385 0.87 -0.3 79,323 + +1.2 79,323 + -0.4 79,325 - +0.4 79,325 - +0.0 79,348 - -0.1 79,348 - +0.2 79,466 + +0.3 79,607 + MMP_RS00390 0.21 -0.1 79,919 + MMP_RS00390 0.46 +0.5 80,013 - MMP_RS00390 0.53 -0.2 80,013 - MMP_RS00390 0.53 -0.6 80,014 + MMP_RS00390 0.53 +1.2 80,072 - MMP_RS00390 0.58 +0.5 80,086 - MMP_RS00390 0.59 +1.6 80,158 - MMP_RS00390 0.65 +0.4 80,158 - MMP_RS00390 0.65 +1.3 80,158 - MMP_RS00390 0.65 +0.6 80,161 + MMP_RS00390 0.65 +0.1 80,161 + MMP_RS00390 0.65 +1.4 80,161 + MMP_RS00390 0.65 +0.0 80,240 + MMP_RS00390 0.72 +0.2 80,279 - MMP_RS00390 0.75 -0.1 80,311 - MMP_RS00390 0.78 -0.1 80,312 + MMP_RS00390 0.78 -0.5
Or see this region's nucleotide sequence