Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14730 and IAI47_14735 overlap by 1 nucleotides IAI47_14735 and IAI47_14740 are separated by 19 nucleotides IAI47_14740 and IAI47_14745 are separated by 81 nucleotides
IAI47_14730: IAI47_14730 - exodeoxyribonuclease VII small subunit, at 3,108,421 to 3,108,663
_14730
IAI47_14735: IAI47_14735 - (2E,6E)-farnesyl diphosphate synthase, at 3,108,663 to 3,109,562
_14735
IAI47_14740: IAI47_14740 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,109,582 to 3,111,447
_14740
IAI47_14745: IAI47_14745 - aldo/keto reductase, at 3,111,529 to 3,112,503
_14745
Position (kb)
3109
3110
3111
3112 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3108.621 kb on + strand, within IAI47_14730 at 3108.621 kb on + strand, within IAI47_14730 at 3109.560 kb on + strand at 3109.560 kb on + strand at 3109.569 kb on + strand at 3111.445 kb on + strand at 3111.446 kb on - strand at 3111.491 kb on + strand at 3111.491 kb on + strand at 3111.491 kb on + strand at 3111.491 kb on + strand at 3111.491 kb on + strand at 3111.492 kb on - strand at 3111.492 kb on - strand at 3111.492 kb on - strand at 3111.492 kb on - strand at 3111.492 kb on - strand at 3111.492 kb on - strand at 3111.492 kb on - strand at 3111.498 kb on + strand at 3111.498 kb on + strand at 3111.499 kb on - strand at 3111.534 kb on + strand at 3111.534 kb on + strand at 3111.534 kb on + strand at 3111.534 kb on + strand at 3111.534 kb on + strand at 3111.535 kb on - strand at 3111.535 kb on - strand at 3111.535 kb on - strand at 3111.535 kb on - strand at 3111.535 kb on - strand at 3111.549 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.588 kb on + strand at 3111.589 kb on - strand at 3111.589 kb on - strand at 3111.589 kb on - strand at 3111.589 kb on - strand at 3111.589 kb on - strand at 3111.595 kb on + strand at 3111.595 kb on + strand at 3111.595 kb on + strand at 3111.595 kb on + strand at 3111.595 kb on + strand at 3111.596 kb on - strand at 3111.656 kb on + strand, within IAI47_14745 at 3111.681 kb on + strand, within IAI47_14745 at 3111.682 kb on - strand, within IAI47_14745 at 3111.682 kb on - strand, within IAI47_14745 at 3111.682 kb on - strand, within IAI47_14745 at 3111.702 kb on + strand, within IAI47_14745 at 3111.703 kb on - strand, within IAI47_14745 at 3111.709 kb on + strand, within IAI47_14745 at 3111.709 kb on + strand, within IAI47_14745 at 3111.709 kb on + strand, within IAI47_14745 at 3111.710 kb on - strand, within IAI47_14745 at 3111.710 kb on - strand, within IAI47_14745 at 3111.710 kb on - strand, within IAI47_14745 at 3111.710 kb on - strand, within IAI47_14745 at 3111.797 kb on - strand, within IAI47_14745 at 3111.797 kb on - strand, within IAI47_14745 at 3111.797 kb on - strand, within IAI47_14745 at 3111.797 kb on - strand, within IAI47_14745 at 3111.810 kb on + strand, within IAI47_14745 at 3111.811 kb on - strand, within IAI47_14745 at 3111.811 kb on - strand, within IAI47_14745 at 3111.811 kb on - strand, within IAI47_14745 at 3111.840 kb on + strand, within IAI47_14745 at 3111.841 kb on - strand, within IAI47_14745 at 3111.886 kb on + strand, within IAI47_14745 at 3111.886 kb on + strand, within IAI47_14745 at 3111.886 kb on + strand, within IAI47_14745 at 3111.886 kb on + strand, within IAI47_14745 at 3111.886 kb on + strand, within IAI47_14745 at 3111.886 kb on + strand, within IAI47_14745 at 3111.887 kb on - strand, within IAI47_14745 at 3111.887 kb on - strand, within IAI47_14745 at 3111.887 kb on - strand, within IAI47_14745 at 3111.919 kb on + strand, within IAI47_14745 at 3111.919 kb on + strand, within IAI47_14745 at 3111.920 kb on - strand, within IAI47_14745 at 3111.920 kb on - strand, within IAI47_14745 at 3111.986 kb on - strand, within IAI47_14745 at 3112.084 kb on + strand, within IAI47_14745 at 3112.085 kb on - strand, within IAI47_14745 at 3112.085 kb on - strand, within IAI47_14745 at 3112.085 kb on - strand, within IAI47_14745 at 3112.232 kb on + strand, within IAI47_14745 at 3112.232 kb on + strand, within IAI47_14745 at 3112.233 kb on - strand, within IAI47_14745 at 3112.233 kb on - strand, within IAI47_14745 at 3112.246 kb on + strand, within IAI47_14745 at 3112.246 kb on + strand, within IAI47_14745 at 3112.246 kb on + strand, within IAI47_14745 at 3112.247 kb on - strand, within IAI47_14745 at 3112.247 kb on - strand, within IAI47_14745 at 3112.247 kb on - strand, within IAI47_14745 at 3112.247 kb on - strand, within IAI47_14745 at 3112.247 kb on - strand, within IAI47_14745 at 3112.247 kb on - strand, within IAI47_14745 at 3112.308 kb on + strand, within IAI47_14745 at 3112.309 kb on - strand, within IAI47_14745 at 3112.309 kb on - strand, within IAI47_14745 at 3112.309 kb on - strand, within IAI47_14745 at 3112.378 kb on - strand, within IAI47_14745
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 3,108,621 + IAI47_14730 0.82 +3.5 3,108,621 + IAI47_14730 0.82 -0.1 3,109,560 + +1.4 3,109,560 + -1.6 3,109,569 + -0.6 3,111,445 + +0.8 3,111,446 - -2.5 3,111,491 + +1.5 3,111,491 + +1.5 3,111,491 + +0.4 3,111,491 + +1.2 3,111,491 + +0.7 3,111,492 - +1.6 3,111,492 - -1.1 3,111,492 - -1.3 3,111,492 - -0.8 3,111,492 - -1.2 3,111,492 - -1.3 3,111,492 - -2.1 3,111,498 + -1.3 3,111,498 + -1.2 3,111,499 - +4.6 3,111,534 + +2.1 3,111,534 + +0.2 3,111,534 + +0.0 3,111,534 + -1.3 3,111,534 + -0.1 3,111,535 - +0.6 3,111,535 - +1.6 3,111,535 - -0.4 3,111,535 - -1.7 3,111,535 - -1.2 3,111,549 + +1.7 3,111,588 + +2.0 3,111,588 + +1.6 3,111,588 + +1.2 3,111,588 + -0.2 3,111,588 + -2.7 3,111,588 + +0.3 3,111,588 + +0.9 3,111,588 + +1.5 3,111,588 + -0.2 3,111,589 - +0.3 3,111,589 - -2.0 3,111,589 - -0.1 3,111,589 - -1.2 3,111,589 - +0.6 3,111,595 + +2.3 3,111,595 + +1.0 3,111,595 + -1.1 3,111,595 + +1.2 3,111,595 + +1.3 3,111,596 - +0.4 3,111,656 + IAI47_14745 0.13 +1.0 3,111,681 + IAI47_14745 0.16 +3.2 3,111,682 - IAI47_14745 0.16 +1.5 3,111,682 - IAI47_14745 0.16 +0.0 3,111,682 - IAI47_14745 0.16 -0.5 3,111,702 + IAI47_14745 0.18 -0.0 3,111,703 - IAI47_14745 0.18 -2.0 3,111,709 + IAI47_14745 0.18 +0.7 3,111,709 + IAI47_14745 0.18 +0.4 3,111,709 + IAI47_14745 0.18 +0.4 3,111,710 - IAI47_14745 0.19 -0.8 3,111,710 - IAI47_14745 0.19 +0.8 3,111,710 - IAI47_14745 0.19 +0.0 3,111,710 - IAI47_14745 0.19 +1.2 3,111,797 - IAI47_14745 0.27 +0.4 3,111,797 - IAI47_14745 0.27 -1.0 3,111,797 - IAI47_14745 0.27 +1.6 3,111,797 - IAI47_14745 0.27 +4.3 3,111,810 + IAI47_14745 0.29 -0.2 3,111,811 - IAI47_14745 0.29 +0.5 3,111,811 - IAI47_14745 0.29 +2.6 3,111,811 - IAI47_14745 0.29 +2.4 3,111,840 + IAI47_14745 0.32 +1.3 3,111,841 - IAI47_14745 0.32 -2.0 3,111,886 + IAI47_14745 0.37 +3.3 3,111,886 + IAI47_14745 0.37 +2.7 3,111,886 + IAI47_14745 0.37 -1.5 3,111,886 + IAI47_14745 0.37 +0.5 3,111,886 + IAI47_14745 0.37 -1.0 3,111,886 + IAI47_14745 0.37 -1.8 3,111,887 - IAI47_14745 0.37 -0.1 3,111,887 - IAI47_14745 0.37 +0.0 3,111,887 - IAI47_14745 0.37 +2.3 3,111,919 + IAI47_14745 0.40 +1.7 3,111,919 + IAI47_14745 0.40 +0.3 3,111,920 - IAI47_14745 0.40 -1.8 3,111,920 - IAI47_14745 0.40 +0.1 3,111,986 - IAI47_14745 0.47 +2.0 3,112,084 + IAI47_14745 0.57 -0.4 3,112,085 - IAI47_14745 0.57 -1.2 3,112,085 - IAI47_14745 0.57 +1.2 3,112,085 - IAI47_14745 0.57 +3.4 3,112,232 + IAI47_14745 0.72 +1.1 3,112,232 + IAI47_14745 0.72 -1.0 3,112,233 - IAI47_14745 0.72 +1.0 3,112,233 - IAI47_14745 0.72 -1.0 3,112,246 + IAI47_14745 0.74 -1.8 3,112,246 + IAI47_14745 0.74 -0.8 3,112,246 + IAI47_14745 0.74 +2.6 3,112,247 - IAI47_14745 0.74 +0.3 3,112,247 - IAI47_14745 0.74 -2.9 3,112,247 - IAI47_14745 0.74 -2.0 3,112,247 - IAI47_14745 0.74 -2.6 3,112,247 - IAI47_14745 0.74 +0.9 3,112,247 - IAI47_14745 0.74 -1.6 3,112,308 + IAI47_14745 0.80 -0.4 3,112,309 - IAI47_14745 0.80 -3.6 3,112,309 - IAI47_14745 0.80 -0.7 3,112,309 - IAI47_14745 0.80 +1.0 3,112,378 - IAI47_14745 0.87 +0.4
Or see this region's nucleotide sequence