Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00620 and IAI47_00625 are separated by 241 nucleotides IAI47_00625 and IAI47_00630 are separated by 19 nucleotides IAI47_00630 and IAI47_00635 are separated by 69 nucleotides IAI47_00635 and IAI47_00640 overlap by 4 nucleotides IAI47_00640 and IAI47_00645 overlap by 4 nucleotides
IAI47_00620: IAI47_00620 - DNA repair protein RadC, at 136,134 to 136,790
_00620
IAI47_00625: IAI47_00625 - 50S ribosomal protein L28, at 137,032 to 137,268
_00625
IAI47_00630: IAI47_00630 - 50S ribosomal protein L33, at 137,288 to 137,455
_00630
IAI47_00635: IAI47_00635 - bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase, at 137,525 to 138,334
_00635
IAI47_00640: IAI47_00640 - pantetheine-phosphate adenylyltransferase, at 138,331 to 138,810
_00640
IAI47_00645: IAI47_00645 - glycosyltransferase family 2 protein, at 138,807 to 139,583
_00645
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 136.525 kb on + strand, within IAI47_00620 at 136.525 kb on + strand, within IAI47_00620 at 136.526 kb on - strand, within IAI47_00620 at 136.526 kb on - strand, within IAI47_00620 at 136.526 kb on - strand, within IAI47_00620 at 136.538 kb on + strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.539 kb on - strand, within IAI47_00620 at 136.553 kb on + strand, within IAI47_00620 at 136.553 kb on + strand, within IAI47_00620 at 136.553 kb on + strand, within IAI47_00620 at 136.553 kb on + strand, within IAI47_00620 at 136.553 kb on + strand, within IAI47_00620 at 136.553 kb on + strand, within IAI47_00620 at 136.554 kb on - strand, within IAI47_00620 at 136.554 kb on - strand, within IAI47_00620 at 136.554 kb on - strand, within IAI47_00620 at 136.554 kb on - strand, within IAI47_00620 at 136.554 kb on - strand, within IAI47_00620 at 136.554 kb on - strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.556 kb on + strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.557 kb on - strand, within IAI47_00620 at 136.559 kb on + strand, within IAI47_00620 at 136.560 kb on - strand, within IAI47_00620 at 136.560 kb on - strand, within IAI47_00620 at 136.560 kb on - strand, within IAI47_00620 at 136.560 kb on - strand, within IAI47_00620 at 136.560 kb on - strand, within IAI47_00620 at 136.560 kb on - strand, within IAI47_00620 at 136.634 kb on + strand, within IAI47_00620 at 136.634 kb on + strand, within IAI47_00620 at 136.634 kb on + strand, within IAI47_00620 at 136.635 kb on - strand, within IAI47_00620 at 136.711 kb on + strand, within IAI47_00620 at 136.711 kb on + strand, within IAI47_00620 at 136.712 kb on - strand, within IAI47_00620 at 136.712 kb on - strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.720 kb on + strand, within IAI47_00620 at 136.721 kb on - strand, within IAI47_00620 at 136.721 kb on - strand, within IAI47_00620 at 136.721 kb on - strand, within IAI47_00620 at 136.721 kb on - strand, within IAI47_00620 at 136.721 kb on - strand, within IAI47_00620 at 136.758 kb on + strand at 136.758 kb on + strand at 136.758 kb on + strand at 136.758 kb on + strand at 136.758 kb on + strand at 136.758 kb on + strand at 136.759 kb on - strand at 136.759 kb on - strand at 136.759 kb on - strand at 136.759 kb on - strand at 136.759 kb on - strand at 136.759 kb on - strand at 136.759 kb on - strand at 136.845 kb on + strand at 136.845 kb on + strand at 136.846 kb on - strand at 136.846 kb on - strand at 136.846 kb on - strand at 137.506 kb on - strand at 137.506 kb on - strand at 137.525 kb on - strand at 137.525 kb on - strand at 137.525 kb on - strand at 137.525 kb on - strand at 137.611 kb on + strand, within IAI47_00635 at 137.611 kb on + strand, within IAI47_00635 at 137.611 kb on + strand, within IAI47_00635 at 137.612 kb on - strand, within IAI47_00635 at 137.612 kb on - strand, within IAI47_00635 at 137.612 kb on - strand, within IAI47_00635 at 137.696 kb on + strand, within IAI47_00635 at 137.697 kb on - strand, within IAI47_00635 at 137.697 kb on - strand, within IAI47_00635 at 137.728 kb on + strand, within IAI47_00635 at 137.728 kb on + strand, within IAI47_00635 at 137.728 kb on + strand, within IAI47_00635 at 137.728 kb on + strand, within IAI47_00635 at 137.729 kb on - strand, within IAI47_00635 at 137.729 kb on - strand, within IAI47_00635 at 137.729 kb on - strand, within IAI47_00635 at 137.729 kb on - strand, within IAI47_00635 at 137.729 kb on - strand, within IAI47_00635 at 137.808 kb on + strand, within IAI47_00635 at 137.808 kb on + strand, within IAI47_00635 at 137.837 kb on + strand, within IAI47_00635 at 137.837 kb on + strand, within IAI47_00635 at 137.838 kb on - strand, within IAI47_00635 at 137.838 kb on - strand, within IAI47_00635 at 137.893 kb on + strand, within IAI47_00635 at 137.893 kb on + strand, within IAI47_00635 at 137.893 kb on + strand, within IAI47_00635 at 137.893 kb on + strand, within IAI47_00635 at 137.893 kb on + strand, within IAI47_00635 at 137.893 kb on - strand, within IAI47_00635 at 137.894 kb on - strand, within IAI47_00635 at 137.948 kb on + strand, within IAI47_00635 at 137.948 kb on + strand, within IAI47_00635 at 137.948 kb on + strand, within IAI47_00635 at 137.949 kb on - strand, within IAI47_00635 at 137.949 kb on - strand, within IAI47_00635 at 137.959 kb on + strand, within IAI47_00635 at 137.959 kb on + strand, within IAI47_00635 at 137.959 kb on + strand, within IAI47_00635 at 137.960 kb on - strand, within IAI47_00635 at 137.986 kb on + strand, within IAI47_00635 at 137.986 kb on + strand, within IAI47_00635 at 137.987 kb on - strand, within IAI47_00635 at 137.987 kb on - strand, within IAI47_00635 at 138.005 kb on - strand, within IAI47_00635 at 138.005 kb on - strand, within IAI47_00635 at 138.005 kb on - strand, within IAI47_00635 at 138.035 kb on + strand, within IAI47_00635 at 138.035 kb on + strand, within IAI47_00635 at 138.035 kb on + strand, within IAI47_00635 at 138.035 kb on + strand, within IAI47_00635 at 138.036 kb on - strand, within IAI47_00635 at 138.124 kb on + strand, within IAI47_00635 at 138.125 kb on - strand, within IAI47_00635 at 138.125 kb on - strand, within IAI47_00635 at 138.125 kb on - strand, within IAI47_00635 at 138.125 kb on - strand, within IAI47_00635 at 138.125 kb on - strand, within IAI47_00635 at 138.125 kb on - strand, within IAI47_00635 at 138.186 kb on + strand, within IAI47_00635 at 138.186 kb on + strand, within IAI47_00635 at 138.187 kb on - strand, within IAI47_00635 at 138.199 kb on + strand, within IAI47_00635 at 138.200 kb on - strand, within IAI47_00635 at 138.200 kb on - strand, within IAI47_00635 at 138.209 kb on + strand, within IAI47_00635 at 138.209 kb on + strand, within IAI47_00635 at 138.209 kb on + strand, within IAI47_00635 at 138.210 kb on - strand, within IAI47_00635 at 138.233 kb on + strand, within IAI47_00635 at 138.233 kb on + strand, within IAI47_00635 at 138.233 kb on + strand, within IAI47_00635 at 138.234 kb on - strand, within IAI47_00635 at 138.234 kb on - strand, within IAI47_00635 at 138.234 kb on - strand, within IAI47_00635 at 138.234 kb on - strand, within IAI47_00635 at 138.234 kb on - strand, within IAI47_00635 at 138.234 kb on - strand, within IAI47_00635 at 138.269 kb on - strand at 138.269 kb on - strand at 138.308 kb on + strand at 138.308 kb on + strand at 138.308 kb on + strand at 138.309 kb on - strand at 138.309 kb on - strand at 138.309 kb on - strand at 138.309 kb on - strand at 138.309 kb on - strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.332 kb on + strand at 138.333 kb on - strand at 138.333 kb on - strand at 138.333 kb on - strand at 138.811 kb on - strand at 138.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 136,525 + IAI47_00620 0.60 -0.8 136,525 + IAI47_00620 0.60 +0.1 136,526 - IAI47_00620 0.60 -0.6 136,526 - IAI47_00620 0.60 +2.2 136,526 - IAI47_00620 0.60 +2.3 136,538 + IAI47_00620 0.61 -0.0 136,539 - IAI47_00620 0.62 +0.0 136,539 - IAI47_00620 0.62 +1.9 136,539 - IAI47_00620 0.62 -0.6 136,539 - IAI47_00620 0.62 +0.2 136,539 - IAI47_00620 0.62 +2.6 136,539 - IAI47_00620 0.62 +1.6 136,539 - IAI47_00620 0.62 +2.3 136,553 + IAI47_00620 0.64 +0.8 136,553 + IAI47_00620 0.64 -1.0 136,553 + IAI47_00620 0.64 -1.3 136,553 + IAI47_00620 0.64 -1.7 136,553 + IAI47_00620 0.64 +0.1 136,553 + IAI47_00620 0.64 +1.3 136,554 - IAI47_00620 0.64 +0.0 136,554 - IAI47_00620 0.64 -0.8 136,554 - IAI47_00620 0.64 -1.6 136,554 - IAI47_00620 0.64 +1.0 136,554 - IAI47_00620 0.64 -2.4 136,554 - IAI47_00620 0.64 -0.8 136,556 + IAI47_00620 0.64 -0.0 136,556 + IAI47_00620 0.64 -0.3 136,556 + IAI47_00620 0.64 -1.3 136,556 + IAI47_00620 0.64 +0.8 136,556 + IAI47_00620 0.64 -2.7 136,556 + IAI47_00620 0.64 +2.2 136,556 + IAI47_00620 0.64 +1.4 136,556 + IAI47_00620 0.64 +1.0 136,556 + IAI47_00620 0.64 -1.7 136,556 + IAI47_00620 0.64 +0.2 136,556 + IAI47_00620 0.64 -1.6 136,556 + IAI47_00620 0.64 +2.1 136,557 - IAI47_00620 0.64 +0.2 136,557 - IAI47_00620 0.64 +2.8 136,557 - IAI47_00620 0.64 -2.0 136,557 - IAI47_00620 0.64 -1.1 136,557 - IAI47_00620 0.64 +0.0 136,557 - IAI47_00620 0.64 -0.6 136,557 - IAI47_00620 0.64 +0.5 136,557 - IAI47_00620 0.64 +0.9 136,557 - IAI47_00620 0.64 +1.4 136,557 - IAI47_00620 0.64 -1.0 136,559 + IAI47_00620 0.65 +0.5 136,560 - IAI47_00620 0.65 +2.9 136,560 - IAI47_00620 0.65 -2.2 136,560 - IAI47_00620 0.65 +1.7 136,560 - IAI47_00620 0.65 -1.8 136,560 - IAI47_00620 0.65 +1.7 136,560 - IAI47_00620 0.65 +1.0 136,634 + IAI47_00620 0.76 +1.7 136,634 + IAI47_00620 0.76 -0.1 136,634 + IAI47_00620 0.76 -0.6 136,635 - IAI47_00620 0.76 +0.2 136,711 + IAI47_00620 0.88 -1.6 136,711 + IAI47_00620 0.88 +0.8 136,712 - IAI47_00620 0.88 -1.2 136,712 - IAI47_00620 0.88 +1.5 136,720 + IAI47_00620 0.89 -1.9 136,720 + IAI47_00620 0.89 -0.1 136,720 + IAI47_00620 0.89 -2.4 136,720 + IAI47_00620 0.89 +0.8 136,720 + IAI47_00620 0.89 -2.7 136,720 + IAI47_00620 0.89 -0.0 136,720 + IAI47_00620 0.89 +0.9 136,720 + IAI47_00620 0.89 +1.5 136,720 + IAI47_00620 0.89 +2.0 136,720 + IAI47_00620 0.89 +1.8 136,721 - IAI47_00620 0.89 +0.9 136,721 - IAI47_00620 0.89 +0.7 136,721 - IAI47_00620 0.89 -1.6 136,721 - IAI47_00620 0.89 -0.6 136,721 - IAI47_00620 0.89 +0.5 136,758 + -1.3 136,758 + +1.3 136,758 + +1.6 136,758 + -0.5 136,758 + -1.4 136,758 + -0.6 136,759 - +0.6 136,759 - +1.7 136,759 - -1.4 136,759 - -0.8 136,759 - -0.5 136,759 - -0.5 136,759 - -0.1 136,845 + -0.4 136,845 + -0.1 136,846 - +1.2 136,846 - +0.6 136,846 - -1.7 137,506 - +0.4 137,506 - -0.8 137,525 - -0.3 137,525 - -1.4 137,525 - +1.4 137,525 - -0.4 137,611 + IAI47_00635 0.11 -0.1 137,611 + IAI47_00635 0.11 -1.7 137,611 + IAI47_00635 0.11 +0.7 137,612 - IAI47_00635 0.11 +0.9 137,612 - IAI47_00635 0.11 -1.0 137,612 - IAI47_00635 0.11 -0.6 137,696 + IAI47_00635 0.21 +0.3 137,697 - IAI47_00635 0.21 -0.1 137,697 - IAI47_00635 0.21 -1.5 137,728 + IAI47_00635 0.25 -2.7 137,728 + IAI47_00635 0.25 -3.3 137,728 + IAI47_00635 0.25 -0.8 137,728 + IAI47_00635 0.25 +0.6 137,729 - IAI47_00635 0.25 -0.2 137,729 - IAI47_00635 0.25 -1.4 137,729 - IAI47_00635 0.25 -0.8 137,729 - IAI47_00635 0.25 +2.2 137,729 - IAI47_00635 0.25 -0.2 137,808 + IAI47_00635 0.35 -1.0 137,808 + IAI47_00635 0.35 +0.2 137,837 + IAI47_00635 0.39 -0.4 137,837 + IAI47_00635 0.39 -1.3 137,838 - IAI47_00635 0.39 +0.8 137,838 - IAI47_00635 0.39 -0.7 137,893 + IAI47_00635 0.45 +0.7 137,893 + IAI47_00635 0.45 -0.6 137,893 + IAI47_00635 0.45 -1.3 137,893 + IAI47_00635 0.45 -0.6 137,893 + IAI47_00635 0.45 +0.3 137,893 - IAI47_00635 0.45 -1.3 137,894 - IAI47_00635 0.46 -1.0 137,948 + IAI47_00635 0.52 +1.3 137,948 + IAI47_00635 0.52 -0.4 137,948 + IAI47_00635 0.52 -1.7 137,949 - IAI47_00635 0.52 -1.1 137,949 - IAI47_00635 0.52 -0.3 137,959 + IAI47_00635 0.54 -1.0 137,959 + IAI47_00635 0.54 +1.5 137,959 + IAI47_00635 0.54 +0.7 137,960 - IAI47_00635 0.54 -1.4 137,986 + IAI47_00635 0.57 -1.0 137,986 + IAI47_00635 0.57 -3.0 137,987 - IAI47_00635 0.57 +1.6 137,987 - IAI47_00635 0.57 +0.2 138,005 - IAI47_00635 0.59 -0.2 138,005 - IAI47_00635 0.59 -0.4 138,005 - IAI47_00635 0.59 -1.2 138,035 + IAI47_00635 0.63 +1.4 138,035 + IAI47_00635 0.63 +0.4 138,035 + IAI47_00635 0.63 -0.2 138,035 + IAI47_00635 0.63 -0.0 138,036 - IAI47_00635 0.63 -1.5 138,124 + IAI47_00635 0.74 -0.0 138,125 - IAI47_00635 0.74 +2.8 138,125 - IAI47_00635 0.74 -1.1 138,125 - IAI47_00635 0.74 -2.3 138,125 - IAI47_00635 0.74 -0.5 138,125 - IAI47_00635 0.74 +1.3 138,125 - IAI47_00635 0.74 -0.3 138,186 + IAI47_00635 0.82 -0.4 138,186 + IAI47_00635 0.82 +5.8 138,187 - IAI47_00635 0.82 -1.8 138,199 + IAI47_00635 0.83 +2.2 138,200 - IAI47_00635 0.83 +1.7 138,200 - IAI47_00635 0.83 +0.1 138,209 + IAI47_00635 0.84 +0.3 138,209 + IAI47_00635 0.84 -1.3 138,209 + IAI47_00635 0.84 -0.8 138,210 - IAI47_00635 0.85 +1.4 138,233 + IAI47_00635 0.87 +2.9 138,233 + IAI47_00635 0.87 -2.1 138,233 + IAI47_00635 0.87 -2.5 138,234 - IAI47_00635 0.88 -1.7 138,234 - IAI47_00635 0.88 -0.1 138,234 - IAI47_00635 0.88 -0.4 138,234 - IAI47_00635 0.88 -0.1 138,234 - IAI47_00635 0.88 -0.3 138,234 - IAI47_00635 0.88 +0.2 138,269 - -1.8 138,269 - -2.3 138,308 + -2.0 138,308 + -3.4 138,308 + -1.3 138,309 - -2.7 138,309 - -0.3 138,309 - +0.5 138,309 - +1.5 138,309 - -1.3 138,332 + -0.4 138,332 + -2.2 138,332 + -1.3 138,332 + -2.4 138,332 + -1.7 138,332 + +0.9 138,332 + +0.6 138,332 + +1.6 138,332 + -0.4 138,333 - +0.4 138,333 - -1.8 138,333 - +0.6 138,811 - +2.0 138,811 - -0.1
Or see this region's nucleotide sequence