Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_65

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_63 and Pf1N1B4_64 are separated by 109 nucleotidesPf1N1B4_64 and Pf1N1B4_65 are separated by 194 nucleotidesPf1N1B4_65 and Pf1N1B4_66 are separated by 61 nucleotidesPf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotides Pf1N1B4_63 - Phosphonoacetaldehyde phosphonohydrolase-related protein, at 66,737 to 67,357 _63 Pf1N1B4_64 - Chromosome segregation ATPases, at 67,467 to 67,730 _64 Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755 _65 Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353 _66 Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186 _67 Position (kb) 67 68 69Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 66.991 kb on + strandat 66.992 kb on - strandat 66.992 kb on - strand, within Pf1N1B4_63at 67.322 kb on - strandat 67.390 kb on - strandat 67.394 kb on + strandat 67.394 kb on + strandat 67.395 kb on - strandat 67.395 kb on - strandat 67.408 kb on - strandat 67.408 kb on - strandat 67.433 kb on + strandat 67.434 kb on - strandat 67.451 kb on + strandat 67.741 kb on + strandat 67.741 kb on + strandat 67.838 kb on + strandat 67.838 kb on + strandat 67.839 kb on - strandat 67.839 kb on - strandat 67.923 kb on + strandat 67.923 kb on + strandat 67.923 kb on + strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.927 kb on - strandat 67.927 kb on - strandat 68.011 kb on - strand, within Pf1N1B4_65at 68.011 kb on - strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.122 kb on - strand, within Pf1N1B4_65at 68.122 kb on - strand, within Pf1N1B4_65at 68.232 kb on - strand, within Pf1N1B4_65at 68.245 kb on + strand, within Pf1N1B4_65at 68.246 kb on - strandat 68.292 kb on + strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.484 kb on + strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strandat 68.620 kb on - strand, within Pf1N1B4_65at 68.620 kb on - strand, within Pf1N1B4_65at 68.698 kb on - strandat 68.698 kb on - strandat 68.709 kb on + strandat 68.710 kb on - strandat 68.871 kb on - strand, within Pf1N1B4_66at 69.038 kb on + strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strandat 69.045 kb on - strand, within Pf1N1B4_66at 69.129 kb on + strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strandat 69.150 kb on + strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.271 kb on + strand, within Pf1N1B4_66at 69.330 kb on + strandat 69.330 kb on + strandat 69.395 kb on + strandat 69.432 kb on + strandat 69.432 kb on + strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.473 kb on + strandat 69.474 kb on - strandat 69.474 kb on - strandat 69.476 kb on - strandat 69.476 kb on - strandat 69.639 kb on - strand, within Pf1N1B4_67at 69.729 kb on + strand, within Pf1N1B4_67

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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66,991 + +0.1
66,992 - +1.9
66,992 - Pf1N1B4_63 0.41 +0.1
67,322 - +0.4
67,390 - -1.1
67,394 + +0.8
67,394 + +0.4
67,395 - -1.4
67,395 - -0.5
67,408 - +0.1
67,408 - +0.4
67,433 + +0.9
67,434 - -2.5
67,451 + -0.2
67,741 + -0.6
67,741 + -0.2
67,838 + -0.1
67,838 + -0.4
67,839 - -0.1
67,839 - +0.9
67,923 + +0.3
67,923 + -0.1
67,923 + -1.8
67,924 - -1.2
67,924 - +0.3
67,924 - -1.1
67,924 - +0.4
67,924 - -0.8
67,924 - +0.5
67,924 - +0.5
67,924 - -0.1
67,927 - -0.5
67,927 - +0.6
68,011 - Pf1N1B4_65 0.10 -0.1
68,011 - Pf1N1B4_65 0.10 -1.0
68,019 + Pf1N1B4_65 0.11 -0.2
68,019 + Pf1N1B4_65 0.11 +0.1
68,019 + Pf1N1B4_65 0.11 -1.4
68,020 - Pf1N1B4_65 0.11 -0.3
68,020 - Pf1N1B4_65 0.11 -3.4
68,020 - Pf1N1B4_65 0.11 +0.4
68,020 - Pf1N1B4_65 0.11 -0.4
68,122 - Pf1N1B4_65 0.24 -2.4
68,122 - Pf1N1B4_65 0.24 -1.2
68,232 - Pf1N1B4_65 0.37 -1.6
68,245 + Pf1N1B4_65 0.39 +1.3
68,246 - +0.9
68,292 + Pf1N1B4_65 0.44 -1.4
68,293 - Pf1N1B4_65 0.44 -1.4
68,293 - Pf1N1B4_65 0.44 -2.6
68,293 - Pf1N1B4_65 0.44 -1.4
68,293 - Pf1N1B4_65 0.44 -1.2
68,293 - Pf1N1B4_65 0.44 +0.1
68,293 - Pf1N1B4_65 0.44 -2.2
68,484 + Pf1N1B4_65 0.67 +0.2
68,485 - Pf1N1B4_65 0.67 -1.5
68,485 - Pf1N1B4_65 0.67 -2.1
68,485 - Pf1N1B4_65 0.67 -0.3
68,619 + Pf1N1B4_65 0.84 -0.1
68,619 + Pf1N1B4_65 0.84 -0.5
68,619 + +1.5
68,620 - Pf1N1B4_65 0.84 +1.7
68,620 - Pf1N1B4_65 0.84 +0.5
68,698 - -2.4
68,698 - -0.7
68,709 + -0.4
68,710 - -0.1
68,871 - Pf1N1B4_66 0.10 -1.1
69,038 + Pf1N1B4_66 0.41 -0.5
69,045 - Pf1N1B4_66 0.42 -0.6
69,045 - Pf1N1B4_66 0.42 -0.6
69,045 - Pf1N1B4_66 0.42 -0.0
69,045 - Pf1N1B4_66 0.42 -0.6
69,045 - +0.2
69,045 - Pf1N1B4_66 0.42 -0.2
69,129 + Pf1N1B4_66 0.58 -0.6
69,130 - Pf1N1B4_66 0.58 -0.5
69,130 - Pf1N1B4_66 0.58 -0.1
69,141 - Pf1N1B4_66 0.60 -0.1
69,141 - Pf1N1B4_66 0.60 +0.7
69,141 - Pf1N1B4_66 0.60 -0.6
69,141 - Pf1N1B4_66 0.60 +0.1
69,141 - +0.4
69,150 + Pf1N1B4_66 0.62 +0.1
69,207 - Pf1N1B4_66 0.73 +0.7
69,207 - Pf1N1B4_66 0.73 -0.3
69,271 + Pf1N1B4_66 0.85 -0.1
69,330 + +0.9
69,330 + -0.7
69,395 + -0.3
69,432 + -0.8
69,432 + -0.4
69,433 - +1.1
69,433 - +0.2
69,433 - -0.1
69,433 - -0.1
69,473 + -0.6
69,474 - -0.7
69,474 - +0.4
69,476 - +0.2
69,476 - -1.1
69,639 - Pf1N1B4_67 0.20 +0.5
69,729 + Pf1N1B4_67 0.33 -1.3

Or see this region's nucleotide sequence