Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4356 and Pf1N1B4_4357 are separated by 421 nucleotides Pf1N1B4_4357 and Pf1N1B4_4358 are separated by 405 nucleotides Pf1N1B4_4358 and Pf1N1B4_4359 are separated by 180 nucleotides
Pf1N1B4_4356 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 4,813,675 to 4,814,973
_4356
Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943
_4357
Pf1N1B4_4358 - Acetyltransferase, GNAT family, at 4,816,349 to 4,816,711
_4358
Pf1N1B4_4359 - PvcA protein, related to known isonitrile synthases, at 4,816,892 to 4,817,887
_4359
Position (kb)
4815
4816 Strain fitness (log2 ratio)
-2
-1
0
1 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.539 kb on + strand, within Pf1N1B4_4356 at 4814.540 kb on - strand, within Pf1N1B4_4356 at 4814.764 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.942 kb on - strand at 4814.967 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.972 kb on - strand at 4814.972 kb on - strand at 4815.017 kb on - strand at 4815.231 kb on - strand at 4815.231 kb on - strand at 4815.287 kb on - strand at 4815.287 kb on - strand at 4815.370 kb on + strand at 4815.370 kb on + strand at 4815.371 kb on - strand at 4815.375 kb on - strand at 4815.421 kb on - strand at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.612 kb on - strand, within Pf1N1B4_4357 at 4815.826 kb on + strand, within Pf1N1B4_4357 at 4816.008 kb on + strand at 4816.169 kb on + strand at 4816.169 kb on + strand at 4816.170 kb on - strand at 4816.170 kb on - strand at 4816.209 kb on - strand at 4816.209 kb on - strand at 4816.334 kb on + strand at 4816.335 kb on - strand at 4816.337 kb on - strand at 4816.386 kb on - strand, within Pf1N1B4_4358 at 4816.538 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.652 kb on + strand, within Pf1N1B4_4358 at 4816.728 kb on + strand at 4816.840 kb on + strand at 4816.892 kb on - strand at 4816.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 4,814,518 + Pf1N1B4_4356 0.65 +0.1 4,814,518 + Pf1N1B4_4356 0.65 -0.3 4,814,519 - Pf1N1B4_4356 0.65 -0.3 4,814,519 - Pf1N1B4_4356 0.65 +0.0 4,814,519 - Pf1N1B4_4356 0.65 +0.1 4,814,539 + Pf1N1B4_4356 0.67 +0.4 4,814,540 - Pf1N1B4_4356 0.67 +0.3 4,814,764 + Pf1N1B4_4356 0.84 +0.6 4,814,767 + +0.1 4,814,767 + Pf1N1B4_4356 0.84 +1.0 4,814,767 + Pf1N1B4_4356 0.84 +0.3 4,814,767 + +0.1 4,814,767 + Pf1N1B4_4356 0.84 -0.4 4,814,767 + Pf1N1B4_4356 0.84 +0.3 4,814,767 + Pf1N1B4_4356 0.84 -0.2 4,814,768 - Pf1N1B4_4356 0.84 +0.4 4,814,768 - Pf1N1B4_4356 0.84 +0.4 4,814,768 - Pf1N1B4_4356 0.84 -1.5 4,814,942 - +0.1 4,814,967 + -0.8 4,814,971 + -0.4 4,814,971 + -0.2 4,814,971 + -1.1 4,814,971 + +0.2 4,814,972 - -0.8 4,814,972 - +0.2 4,815,017 - -0.4 4,815,231 - -1.2 4,815,231 - -0.1 4,815,287 - -1.2 4,815,287 - +1.0 4,815,370 + +0.8 4,815,370 + +0.3 4,815,371 - -0.2 4,815,375 - -1.3 4,815,421 - +0.1 4,815,611 + Pf1N1B4_4357 0.39 -1.4 4,815,611 + Pf1N1B4_4357 0.39 -1.8 4,815,611 + Pf1N1B4_4357 0.39 +0.4 4,815,611 + Pf1N1B4_4357 0.39 +0.6 4,815,612 - Pf1N1B4_4357 0.40 -2.3 4,815,826 + Pf1N1B4_4357 0.79 -1.2 4,816,008 + -0.5 4,816,169 + +0.1 4,816,169 + -0.5 4,816,170 - +0.4 4,816,170 - -0.1 4,816,209 - -0.1 4,816,209 - -0.9 4,816,334 + -0.2 4,816,335 - -0.6 4,816,337 - +1.5 4,816,386 - Pf1N1B4_4358 0.10 +1.2 4,816,538 + Pf1N1B4_4358 0.52 -1.2 4,816,648 + Pf1N1B4_4358 0.82 +0.1 4,816,648 + Pf1N1B4_4358 0.82 -1.8 4,816,652 + Pf1N1B4_4358 0.83 +0.3 4,816,728 + -0.5 4,816,840 + -0.2 4,816,892 - -0.5 4,816,892 - +0.7
Or see this region's nucleotide sequence