Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1326

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1325 and Pf1N1B4_1326 are separated by 876 nucleotidesPf1N1B4_1326 and Pf1N1B4_1327 overlap by 1 nucleotidesPf1N1B4_1327 and Pf1N1B4_1328 overlap by 4 nucleotides Pf1N1B4_1325 - Toxin TcdB, at 1,515,373 to 1,519,848 _1325 Pf1N1B4_1326 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 1,520,725 to 1,522,428 _1326 Pf1N1B4_1327 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 1,522,428 to 1,523,162 _1327 Pf1N1B4_1328 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 1,523,159 to 1,523,785 _1328 Position (kb) 1520 1521 1522 1523Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1519.805 kb on + strandat 1519.805 kb on + strandat 1519.806 kb on - strandat 1519.806 kb on - strandat 1519.806 kb on - strandat 1519.806 kb on - strandat 1519.849 kb on - strandat 1519.849 kb on - strandat 1519.962 kb on + strandat 1519.963 kb on - strandat 1519.995 kb on - strandat 1519.995 kb on - strandat 1520.130 kb on - strandat 1520.203 kb on + strandat 1520.254 kb on + strandat 1520.318 kb on + strandat 1520.318 kb on + strandat 1520.319 kb on - strandat 1520.380 kb on - strandat 1520.424 kb on + strandat 1520.502 kb on + strandat 1520.762 kb on + strandat 1520.762 kb on + strandat 1520.762 kb on + strandat 1520.763 kb on - strandat 1520.763 kb on - strandat 1520.763 kb on - strandat 1520.763 kb on - strandat 1520.763 kb on - strandat 1521.002 kb on + strand, within Pf1N1B4_1326at 1521.002 kb on + strand, within Pf1N1B4_1326at 1521.002 kb on + strand, within Pf1N1B4_1326at 1521.002 kb on + strand, within Pf1N1B4_1326at 1521.002 kb on + strand, within Pf1N1B4_1326at 1521.003 kb on - strand, within Pf1N1B4_1326at 1521.003 kb on - strand, within Pf1N1B4_1326at 1521.003 kb on - strand, within Pf1N1B4_1326at 1521.386 kb on + strand, within Pf1N1B4_1326at 1521.386 kb on + strand, within Pf1N1B4_1326at 1521.386 kb on + strand, within Pf1N1B4_1326at 1521.386 kb on + strand, within Pf1N1B4_1326at 1521.386 kb on + strandat 1521.386 kb on + strand, within Pf1N1B4_1326at 1521.386 kb on + strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.387 kb on - strand, within Pf1N1B4_1326at 1521.742 kb on + strandat 1521.743 kb on - strand, within Pf1N1B4_1326at 1521.743 kb on - strand, within Pf1N1B4_1326at 1521.813 kb on - strand, within Pf1N1B4_1326at 1522.054 kb on - strand, within Pf1N1B4_1326at 1522.263 kb on - strandat 1522.296 kb on - strandat 1522.352 kb on + strandat 1522.471 kb on + strandat 1522.543 kb on + strand, within Pf1N1B4_1327at 1522.543 kb on + strand, within Pf1N1B4_1327at 1522.544 kb on - strand, within Pf1N1B4_1327at 1522.778 kb on - strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strandat 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strandat 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.819 kb on + strand, within Pf1N1B4_1327at 1522.820 kb on - strand, within Pf1N1B4_1327at 1522.903 kb on + strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.928 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.361 kb on + strandat 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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1,519,805 + +0.3
1,519,805 + -1.3
1,519,806 - +0.2
1,519,806 - -0.3
1,519,806 - +0.4
1,519,806 - +0.0
1,519,849 - +1.4
1,519,849 - +1.4
1,519,962 + +0.4
1,519,963 - +1.7
1,519,995 - +1.4
1,519,995 - -0.6
1,520,130 - -0.2
1,520,203 + +0.4
1,520,254 + -0.1
1,520,318 + +0.6
1,520,318 + +2.7
1,520,319 - +0.3
1,520,380 - -0.6
1,520,424 + +0.0
1,520,502 + +0.1
1,520,762 + -0.6
1,520,762 + -1.8
1,520,762 + -0.8
1,520,763 - -0.0
1,520,763 - -1.3
1,520,763 - +0.5
1,520,763 - -0.4
1,520,763 - -0.9
1,521,002 + Pf1N1B4_1326 0.16 -0.1
1,521,002 + Pf1N1B4_1326 0.16 -0.8
1,521,002 + Pf1N1B4_1326 0.16 +1.1
1,521,002 + Pf1N1B4_1326 0.16 -0.9
1,521,002 + Pf1N1B4_1326 0.16 -1.1
1,521,003 - Pf1N1B4_1326 0.16 -1.4
1,521,003 - Pf1N1B4_1326 0.16 -2.1
1,521,003 - Pf1N1B4_1326 0.16 +0.1
1,521,386 + Pf1N1B4_1326 0.39 -0.8
1,521,386 + Pf1N1B4_1326 0.39 +0.1
1,521,386 + Pf1N1B4_1326 0.39 +0.6
1,521,386 + Pf1N1B4_1326 0.39 -0.7
1,521,386 + -0.9
1,521,386 + Pf1N1B4_1326 0.39 -1.4
1,521,386 + Pf1N1B4_1326 0.39 -0.6
1,521,387 - Pf1N1B4_1326 0.39 -0.5
1,521,387 - Pf1N1B4_1326 0.39 -0.9
1,521,387 - Pf1N1B4_1326 0.39 +0.2
1,521,387 - Pf1N1B4_1326 0.39 -0.5
1,521,387 - Pf1N1B4_1326 0.39 -1.9
1,521,387 - Pf1N1B4_1326 0.39 -2.1
1,521,387 - Pf1N1B4_1326 0.39 -0.9
1,521,387 - Pf1N1B4_1326 0.39 -2.1
1,521,387 - Pf1N1B4_1326 0.39 -1.4
1,521,387 - Pf1N1B4_1326 0.39 -2.5
1,521,387 - Pf1N1B4_1326 0.39 -1.2
1,521,742 + -1.2
1,521,743 - Pf1N1B4_1326 0.60 +2.8
1,521,743 - Pf1N1B4_1326 0.60 -2.5
1,521,813 - Pf1N1B4_1326 0.64 -0.3
1,522,054 - Pf1N1B4_1326 0.78 -1.9
1,522,263 - -1.2
1,522,296 - -1.1
1,522,352 + -1.1
1,522,471 + -0.5
1,522,543 + Pf1N1B4_1327 0.16 -1.9
1,522,543 + Pf1N1B4_1327 0.16 -1.1
1,522,544 - Pf1N1B4_1327 0.16 -1.6
1,522,778 - Pf1N1B4_1327 0.48 -0.8
1,522,804 + Pf1N1B4_1327 0.51 -1.4
1,522,804 + Pf1N1B4_1327 0.51 +0.7
1,522,804 + -0.6
1,522,804 + Pf1N1B4_1327 0.51 -0.7
1,522,804 + Pf1N1B4_1327 0.51 -2.2
1,522,804 + Pf1N1B4_1327 0.51 -2.3
1,522,805 - Pf1N1B4_1327 0.51 +0.4
1,522,805 - Pf1N1B4_1327 0.51 -0.6
1,522,805 - Pf1N1B4_1327 0.51 +0.2
1,522,805 - Pf1N1B4_1327 0.51 -0.8
1,522,805 - Pf1N1B4_1327 0.51 -0.4
1,522,805 - Pf1N1B4_1327 0.51 +1.1
1,522,805 - Pf1N1B4_1327 0.51 -1.5
1,522,805 - +0.1
1,522,805 - Pf1N1B4_1327 0.51 -1.6
1,522,810 + Pf1N1B4_1327 0.52 -1.4
1,522,810 + Pf1N1B4_1327 0.52 +0.1
1,522,810 + Pf1N1B4_1327 0.52 -0.2
1,522,810 + Pf1N1B4_1327 0.52 -1.2
1,522,810 + Pf1N1B4_1327 0.52 +0.6
1,522,810 + Pf1N1B4_1327 0.52 -0.4
1,522,810 + Pf1N1B4_1327 0.52 -0.4
1,522,810 + Pf1N1B4_1327 0.52 +0.7
1,522,810 + Pf1N1B4_1327 0.52 +0.1
1,522,810 + Pf1N1B4_1327 0.52 -1.8
1,522,810 + Pf1N1B4_1327 0.52 -2.2
1,522,810 + Pf1N1B4_1327 0.52 -2.7
1,522,811 - Pf1N1B4_1327 0.52 +0.2
1,522,811 - Pf1N1B4_1327 0.52 +0.7
1,522,811 - Pf1N1B4_1327 0.52 -1.6
1,522,811 - Pf1N1B4_1327 0.52 -0.9
1,522,811 - Pf1N1B4_1327 0.52 -1.2
1,522,811 - Pf1N1B4_1327 0.52 -1.6
1,522,811 - Pf1N1B4_1327 0.52 -1.1
1,522,811 - Pf1N1B4_1327 0.52 -2.5
1,522,811 - Pf1N1B4_1327 0.52 -1.5
1,522,811 - Pf1N1B4_1327 0.52 -1.2
1,522,811 - Pf1N1B4_1327 0.52 -0.9
1,522,811 - Pf1N1B4_1327 0.52 -2.4
1,522,811 - Pf1N1B4_1327 0.52 -2.1
1,522,819 + Pf1N1B4_1327 0.53 -1.2
1,522,820 - Pf1N1B4_1327 0.53 +0.6
1,522,903 + Pf1N1B4_1327 0.65 -1.3
1,522,904 - Pf1N1B4_1327 0.65 -1.4
1,522,904 - Pf1N1B4_1327 0.65 -1.0
1,522,904 - Pf1N1B4_1327 0.65 -1.6
1,522,904 - Pf1N1B4_1327 0.65 -1.3
1,522,928 - Pf1N1B4_1327 0.68 -0.4
1,523,054 - Pf1N1B4_1327 0.85 -0.9
1,523,054 - Pf1N1B4_1327 0.85 -2.1
1,523,054 - Pf1N1B4_1327 0.85 -1.4
1,523,054 - Pf1N1B4_1327 0.85 -0.3
1,523,361 + -0.6
1,523,361 + Pf1N1B4_1328 0.32 -2.1
1,523,361 + Pf1N1B4_1328 0.32 -0.9
1,523,361 + Pf1N1B4_1328 0.32 -0.2
1,523,362 - Pf1N1B4_1328 0.32 -1.0
1,523,362 - Pf1N1B4_1328 0.32 -1.8
1,523,362 - Pf1N1B4_1328 0.32 -2.7
1,523,362 - Pf1N1B4_1328 0.32 -1.3

Or see this region's nucleotide sequence