Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1325 and Pf1N1B4_1326 are separated by 876 nucleotides Pf1N1B4_1326 and Pf1N1B4_1327 overlap by 1 nucleotides Pf1N1B4_1327 and Pf1N1B4_1328 overlap by 4 nucleotides
Pf1N1B4_1325 - Toxin TcdB, at 1,515,373 to 1,519,848
_1325
Pf1N1B4_1326 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 1,520,725 to 1,522,428
_1326
Pf1N1B4_1327 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 1,522,428 to 1,523,162
_1327
Pf1N1B4_1328 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 1,523,159 to 1,523,785
_1328
Position (kb)
1520
1521
1522
1523 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1519.805 kb on + strand at 1519.805 kb on + strand at 1519.806 kb on - strand at 1519.806 kb on - strand at 1519.806 kb on - strand at 1519.806 kb on - strand at 1519.849 kb on - strand at 1519.849 kb on - strand at 1519.962 kb on + strand at 1519.963 kb on - strand at 1519.995 kb on - strand at 1519.995 kb on - strand at 1520.130 kb on - strand at 1520.203 kb on + strand at 1520.254 kb on + strand at 1520.318 kb on + strand at 1520.318 kb on + strand at 1520.319 kb on - strand at 1520.380 kb on - strand at 1520.424 kb on + strand at 1520.502 kb on + strand at 1520.762 kb on + strand at 1520.762 kb on + strand at 1520.762 kb on + strand at 1520.763 kb on - strand at 1520.763 kb on - strand at 1520.763 kb on - strand at 1520.763 kb on - strand at 1520.763 kb on - strand at 1521.002 kb on + strand, within Pf1N1B4_1326 at 1521.002 kb on + strand, within Pf1N1B4_1326 at 1521.002 kb on + strand, within Pf1N1B4_1326 at 1521.002 kb on + strand, within Pf1N1B4_1326 at 1521.002 kb on + strand, within Pf1N1B4_1326 at 1521.003 kb on - strand, within Pf1N1B4_1326 at 1521.003 kb on - strand, within Pf1N1B4_1326 at 1521.003 kb on - strand, within Pf1N1B4_1326 at 1521.386 kb on + strand, within Pf1N1B4_1326 at 1521.386 kb on + strand, within Pf1N1B4_1326 at 1521.386 kb on + strand, within Pf1N1B4_1326 at 1521.386 kb on + strand, within Pf1N1B4_1326 at 1521.386 kb on + strand at 1521.386 kb on + strand, within Pf1N1B4_1326 at 1521.386 kb on + strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.387 kb on - strand, within Pf1N1B4_1326 at 1521.742 kb on + strand at 1521.743 kb on - strand, within Pf1N1B4_1326 at 1521.743 kb on - strand, within Pf1N1B4_1326 at 1521.813 kb on - strand, within Pf1N1B4_1326 at 1522.054 kb on - strand, within Pf1N1B4_1326 at 1522.263 kb on - strand at 1522.296 kb on - strand at 1522.352 kb on + strand at 1522.471 kb on + strand at 1522.543 kb on + strand, within Pf1N1B4_1327 at 1522.543 kb on + strand, within Pf1N1B4_1327 at 1522.544 kb on - strand, within Pf1N1B4_1327 at 1522.778 kb on - strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.819 kb on + strand, within Pf1N1B4_1327 at 1522.820 kb on - strand, within Pf1N1B4_1327 at 1522.903 kb on + strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.928 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.361 kb on + strand at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 1,519,805 + +0.3 1,519,805 + -1.3 1,519,806 - +0.2 1,519,806 - -0.3 1,519,806 - +0.4 1,519,806 - +0.0 1,519,849 - +1.4 1,519,849 - +1.4 1,519,962 + +0.4 1,519,963 - +1.7 1,519,995 - +1.4 1,519,995 - -0.6 1,520,130 - -0.2 1,520,203 + +0.4 1,520,254 + -0.1 1,520,318 + +0.6 1,520,318 + +2.7 1,520,319 - +0.3 1,520,380 - -0.6 1,520,424 + +0.0 1,520,502 + +0.1 1,520,762 + -0.6 1,520,762 + -1.8 1,520,762 + -0.8 1,520,763 - -0.0 1,520,763 - -1.3 1,520,763 - +0.5 1,520,763 - -0.4 1,520,763 - -0.9 1,521,002 + Pf1N1B4_1326 0.16 -0.1 1,521,002 + Pf1N1B4_1326 0.16 -0.8 1,521,002 + Pf1N1B4_1326 0.16 +1.1 1,521,002 + Pf1N1B4_1326 0.16 -0.9 1,521,002 + Pf1N1B4_1326 0.16 -1.1 1,521,003 - Pf1N1B4_1326 0.16 -1.4 1,521,003 - Pf1N1B4_1326 0.16 -2.1 1,521,003 - Pf1N1B4_1326 0.16 +0.1 1,521,386 + Pf1N1B4_1326 0.39 -0.8 1,521,386 + Pf1N1B4_1326 0.39 +0.1 1,521,386 + Pf1N1B4_1326 0.39 +0.6 1,521,386 + Pf1N1B4_1326 0.39 -0.7 1,521,386 + -0.9 1,521,386 + Pf1N1B4_1326 0.39 -1.4 1,521,386 + Pf1N1B4_1326 0.39 -0.6 1,521,387 - Pf1N1B4_1326 0.39 -0.5 1,521,387 - Pf1N1B4_1326 0.39 -0.9 1,521,387 - Pf1N1B4_1326 0.39 +0.2 1,521,387 - Pf1N1B4_1326 0.39 -0.5 1,521,387 - Pf1N1B4_1326 0.39 -1.9 1,521,387 - Pf1N1B4_1326 0.39 -2.1 1,521,387 - Pf1N1B4_1326 0.39 -0.9 1,521,387 - Pf1N1B4_1326 0.39 -2.1 1,521,387 - Pf1N1B4_1326 0.39 -1.4 1,521,387 - Pf1N1B4_1326 0.39 -2.5 1,521,387 - Pf1N1B4_1326 0.39 -1.2 1,521,742 + -1.2 1,521,743 - Pf1N1B4_1326 0.60 +2.8 1,521,743 - Pf1N1B4_1326 0.60 -2.5 1,521,813 - Pf1N1B4_1326 0.64 -0.3 1,522,054 - Pf1N1B4_1326 0.78 -1.9 1,522,263 - -1.2 1,522,296 - -1.1 1,522,352 + -1.1 1,522,471 + -0.5 1,522,543 + Pf1N1B4_1327 0.16 -1.9 1,522,543 + Pf1N1B4_1327 0.16 -1.1 1,522,544 - Pf1N1B4_1327 0.16 -1.6 1,522,778 - Pf1N1B4_1327 0.48 -0.8 1,522,804 + Pf1N1B4_1327 0.51 -1.4 1,522,804 + Pf1N1B4_1327 0.51 +0.7 1,522,804 + -0.6 1,522,804 + Pf1N1B4_1327 0.51 -0.7 1,522,804 + Pf1N1B4_1327 0.51 -2.2 1,522,804 + Pf1N1B4_1327 0.51 -2.3 1,522,805 - Pf1N1B4_1327 0.51 +0.4 1,522,805 - Pf1N1B4_1327 0.51 -0.6 1,522,805 - Pf1N1B4_1327 0.51 +0.2 1,522,805 - Pf1N1B4_1327 0.51 -0.8 1,522,805 - Pf1N1B4_1327 0.51 -0.4 1,522,805 - Pf1N1B4_1327 0.51 +1.1 1,522,805 - Pf1N1B4_1327 0.51 -1.5 1,522,805 - +0.1 1,522,805 - Pf1N1B4_1327 0.51 -1.6 1,522,810 + Pf1N1B4_1327 0.52 -1.4 1,522,810 + Pf1N1B4_1327 0.52 +0.1 1,522,810 + Pf1N1B4_1327 0.52 -0.2 1,522,810 + Pf1N1B4_1327 0.52 -1.2 1,522,810 + Pf1N1B4_1327 0.52 +0.6 1,522,810 + Pf1N1B4_1327 0.52 -0.4 1,522,810 + Pf1N1B4_1327 0.52 -0.4 1,522,810 + Pf1N1B4_1327 0.52 +0.7 1,522,810 + Pf1N1B4_1327 0.52 +0.1 1,522,810 + Pf1N1B4_1327 0.52 -1.8 1,522,810 + Pf1N1B4_1327 0.52 -2.2 1,522,810 + Pf1N1B4_1327 0.52 -2.7 1,522,811 - Pf1N1B4_1327 0.52 +0.2 1,522,811 - Pf1N1B4_1327 0.52 +0.7 1,522,811 - Pf1N1B4_1327 0.52 -1.6 1,522,811 - Pf1N1B4_1327 0.52 -0.9 1,522,811 - Pf1N1B4_1327 0.52 -1.2 1,522,811 - Pf1N1B4_1327 0.52 -1.6 1,522,811 - Pf1N1B4_1327 0.52 -1.1 1,522,811 - Pf1N1B4_1327 0.52 -2.5 1,522,811 - Pf1N1B4_1327 0.52 -1.5 1,522,811 - Pf1N1B4_1327 0.52 -1.2 1,522,811 - Pf1N1B4_1327 0.52 -0.9 1,522,811 - Pf1N1B4_1327 0.52 -2.4 1,522,811 - Pf1N1B4_1327 0.52 -2.1 1,522,819 + Pf1N1B4_1327 0.53 -1.2 1,522,820 - Pf1N1B4_1327 0.53 +0.6 1,522,903 + Pf1N1B4_1327 0.65 -1.3 1,522,904 - Pf1N1B4_1327 0.65 -1.4 1,522,904 - Pf1N1B4_1327 0.65 -1.0 1,522,904 - Pf1N1B4_1327 0.65 -1.6 1,522,904 - Pf1N1B4_1327 0.65 -1.3 1,522,928 - Pf1N1B4_1327 0.68 -0.4 1,523,054 - Pf1N1B4_1327 0.85 -0.9 1,523,054 - Pf1N1B4_1327 0.85 -2.1 1,523,054 - Pf1N1B4_1327 0.85 -1.4 1,523,054 - Pf1N1B4_1327 0.85 -0.3 1,523,361 + -0.6 1,523,361 + Pf1N1B4_1328 0.32 -2.1 1,523,361 + Pf1N1B4_1328 0.32 -0.9 1,523,361 + Pf1N1B4_1328 0.32 -0.2 1,523,362 - Pf1N1B4_1328 0.32 -1.0 1,523,362 - Pf1N1B4_1328 0.32 -1.8 1,523,362 - Pf1N1B4_1328 0.32 -2.7 1,523,362 - Pf1N1B4_1328 0.32 -1.3
Or see this region's nucleotide sequence