Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05150 and MMP_RS05155 are separated by 157 nucleotides MMP_RS05155 and MMP_RS05160 are separated by 231 nucleotides MMP_RS05160 and MMP_RS05165 are separated by 297 nucleotides
MMP_RS05150: MMP_RS05150 - hypothetical protein, at 981,799 to 982,230
_RS05150
MMP_RS05155: MMP_RS05155 - hypothetical protein, at 982,388 to 982,651
_RS05155
MMP_RS05160: MMP_RS05160 - cupredoxin family copper-binding protein, at 982,883 to 983,239
_RS05160
MMP_RS05165: MMP_RS05165 - right-handed parallel beta-helix repeat-containing protein, at 983,537 to 984,193
_RS05165
Position (kb) 982
983
984 Strain fitness (log2 ratio) -2
-1
0
1
2 at 981.887 kb on - strand, within MMP_RS05150 at 981.888 kb on + strand, within MMP_RS05150 at 981.924 kb on + strand, within MMP_RS05150 at 981.989 kb on + strand, within MMP_RS05150 at 981.991 kb on - strand, within MMP_RS05150 at 981.991 kb on - strand, within MMP_RS05150 at 982.124 kb on - strand, within MMP_RS05150 at 982.125 kb on + strand, within MMP_RS05150 at 982.316 kb on - strand at 982.360 kb on - strand at 982.435 kb on + strand, within MMP_RS05155 at 982.435 kb on + strand, within MMP_RS05155 at 982.502 kb on - strand, within MMP_RS05155 at 982.502 kb on - strand, within MMP_RS05155 at 982.503 kb on + strand, within MMP_RS05155 at 982.611 kb on + strand, within MMP_RS05155 at 982.611 kb on + strand, within MMP_RS05155 at 982.611 kb on + strand, within MMP_RS05155 at 982.666 kb on - strand at 982.667 kb on + strand at 982.667 kb on + strand at 982.727 kb on + strand at 982.773 kb on + strand at 982.830 kb on - strand at 982.852 kb on - strand at 982.874 kb on + strand at 982.914 kb on - strand at 982.915 kb on + strand at 982.928 kb on + strand, within MMP_RS05160 at 982.928 kb on + strand, within MMP_RS05160 at 982.928 kb on + strand, within MMP_RS05160 at 982.932 kb on - strand, within MMP_RS05160 at 982.936 kb on + strand, within MMP_RS05160 at 982.940 kb on + strand, within MMP_RS05160 at 982.940 kb on + strand, within MMP_RS05160 at 982.941 kb on - strand, within MMP_RS05160 at 982.942 kb on + strand, within MMP_RS05160 at 982.942 kb on + strand, within MMP_RS05160 at 982.942 kb on + strand, within MMP_RS05160 at 982.949 kb on - strand, within MMP_RS05160 at 982.963 kb on - strand, within MMP_RS05160 at 982.964 kb on + strand, within MMP_RS05160 at 983.007 kb on + strand, within MMP_RS05160 at 983.024 kb on - strand, within MMP_RS05160 at 983.024 kb on - strand, within MMP_RS05160 at 983.156 kb on + strand, within MMP_RS05160 at 983.217 kb on + strand at 983.219 kb on - strand at 983.220 kb on + strand at 983.220 kb on + strand at 983.265 kb on + strand at 983.265 kb on + strand at 983.285 kb on - strand at 983.291 kb on - strand at 983.313 kb on - strand at 983.314 kb on + strand at 983.319 kb on + strand at 983.324 kb on + strand at 983.341 kb on - strand at 983.342 kb on + strand at 983.412 kb on - strand at 983.583 kb on - strand at 983.595 kb on - strand at 983.646 kb on - strand, within MMP_RS05165 at 983.646 kb on - strand, within MMP_RS05165 at 983.646 kb on - strand, within MMP_RS05165 at 983.646 kb on - strand, within MMP_RS05165 at 983.647 kb on + strand, within MMP_RS05165 at 983.686 kb on - strand, within MMP_RS05165 at 983.686 kb on - strand, within MMP_RS05165 at 983.698 kb on + strand, within MMP_RS05165 at 983.698 kb on + strand, within MMP_RS05165 at 983.700 kb on - strand, within MMP_RS05165 at 983.701 kb on + strand, within MMP_RS05165 at 983.717 kb on - strand, within MMP_RS05165 at 983.754 kb on - strand, within MMP_RS05165 at 983.798 kb on + strand, within MMP_RS05165 at 983.801 kb on + strand, within MMP_RS05165 at 983.804 kb on + strand, within MMP_RS05165 at 983.826 kb on - strand, within MMP_RS05165 at 983.844 kb on - strand, within MMP_RS05165 at 983.977 kb on + strand, within MMP_RS05165 at 983.988 kb on - strand, within MMP_RS05165 at 983.990 kb on - strand, within MMP_RS05165 at 983.990 kb on - strand, within MMP_RS05165 at 983.991 kb on + strand, within MMP_RS05165 at 984.011 kb on - strand, within MMP_RS05165 at 984.011 kb on - strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.016 kb on + strand, within MMP_RS05165 at 984.016 kb on + strand, within MMP_RS05165 at 984.016 kb on + strand, within MMP_RS05165 at 984.017 kb on - strand, within MMP_RS05165 at 984.020 kb on + strand, within MMP_RS05165 at 984.020 kb on + strand, within MMP_RS05165 at 984.083 kb on - strand, within MMP_RS05165 at 984.084 kb on + strand, within MMP_RS05165 at 984.093 kb on - strand, within MMP_RS05165 at 984.093 kb on - strand, within MMP_RS05165 at 984.159 kb on - strand at 984.160 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 981,887 - MMP_RS05150 0.20 +1.3 981,888 + MMP_RS05150 0.21 +1.6 981,924 + MMP_RS05150 0.29 +0.9 981,989 + MMP_RS05150 0.44 -0.3 981,991 - MMP_RS05150 0.44 +0.1 981,991 - MMP_RS05150 0.44 -0.8 982,124 - MMP_RS05150 0.75 -0.5 982,125 + MMP_RS05150 0.75 +0.0 982,316 - -0.2 982,360 - +0.1 982,435 + MMP_RS05155 0.18 -0.1 982,435 + MMP_RS05155 0.18 -0.5 982,502 - MMP_RS05155 0.43 +0.8 982,502 - MMP_RS05155 0.43 +0.2 982,503 + MMP_RS05155 0.44 +0.7 982,611 + MMP_RS05155 0.84 +0.3 982,611 + MMP_RS05155 0.84 +0.4 982,611 + MMP_RS05155 0.84 -0.9 982,666 - -0.1 982,667 + -0.8 982,667 + -0.2 982,727 + -0.3 982,773 + +0.1 982,830 - +1.4 982,852 - +1.2 982,874 + +0.8 982,914 - +0.4 982,915 + +0.1 982,928 + MMP_RS05160 0.13 +0.4 982,928 + MMP_RS05160 0.13 -0.3 982,928 + MMP_RS05160 0.13 -0.2 982,932 - MMP_RS05160 0.14 +0.9 982,936 + MMP_RS05160 0.15 -1.3 982,940 + MMP_RS05160 0.16 -0.1 982,940 + MMP_RS05160 0.16 -1.5 982,941 - MMP_RS05160 0.16 -0.1 982,942 + MMP_RS05160 0.17 -1.3 982,942 + MMP_RS05160 0.17 -2.5 982,942 + MMP_RS05160 0.17 -0.3 982,949 - MMP_RS05160 0.18 -0.6 982,963 - MMP_RS05160 0.22 +0.0 982,964 + MMP_RS05160 0.23 -1.5 983,007 + MMP_RS05160 0.35 -0.2 983,024 - MMP_RS05160 0.39 -0.2 983,024 - MMP_RS05160 0.39 +0.2 983,156 + MMP_RS05160 0.76 -0.2 983,217 + -1.2 983,219 - +0.5 983,220 + -0.4 983,220 + +0.5 983,265 + +2.6 983,265 + +0.1 983,285 - +0.1 983,291 - -1.2 983,313 - +0.7 983,314 + -1.7 983,319 + +1.4 983,324 + +0.2 983,341 - -0.9 983,342 + +0.5 983,412 - +0.5 983,583 - -0.4 983,595 - -1.8 983,646 - MMP_RS05165 0.17 -0.1 983,646 - MMP_RS05165 0.17 -0.5 983,646 - MMP_RS05165 0.17 +0.2 983,646 - MMP_RS05165 0.17 -0.6 983,647 + MMP_RS05165 0.17 -0.6 983,686 - MMP_RS05165 0.23 +2.4 983,686 - MMP_RS05165 0.23 +0.1 983,698 + MMP_RS05165 0.25 -1.2 983,698 + MMP_RS05165 0.25 -0.4 983,700 - MMP_RS05165 0.25 -1.9 983,701 + MMP_RS05165 0.25 -1.0 983,717 - MMP_RS05165 0.27 +0.8 983,754 - MMP_RS05165 0.33 -0.0 983,798 + MMP_RS05165 0.40 -0.1 983,801 + MMP_RS05165 0.40 -0.4 983,804 + MMP_RS05165 0.41 -0.1 983,826 - MMP_RS05165 0.44 -0.6 983,844 - MMP_RS05165 0.47 +0.7 983,977 + MMP_RS05165 0.67 +1.3 983,988 - MMP_RS05165 0.69 -0.1 983,990 - MMP_RS05165 0.69 -0.8 983,990 - MMP_RS05165 0.69 +0.2 983,991 + MMP_RS05165 0.69 -1.4 984,011 - MMP_RS05165 0.72 +0.1 984,011 - MMP_RS05165 0.72 -0.1 984,012 + MMP_RS05165 0.72 +1.4 984,012 + MMP_RS05165 0.72 -0.0 984,012 + MMP_RS05165 0.72 -0.1 984,012 + MMP_RS05165 0.72 +0.3 984,015 - MMP_RS05165 0.73 +1.3 984,015 - MMP_RS05165 0.73 -0.0 984,015 - MMP_RS05165 0.73 +0.8 984,015 - MMP_RS05165 0.73 -1.5 984,016 + MMP_RS05165 0.73 -0.5 984,016 + MMP_RS05165 0.73 -0.0 984,016 + MMP_RS05165 0.73 -1.7 984,017 - MMP_RS05165 0.73 -0.5 984,020 + MMP_RS05165 0.74 +1.0 984,020 + MMP_RS05165 0.74 +0.2 984,083 - MMP_RS05165 0.83 +0.2 984,084 + MMP_RS05165 0.83 -1.1 984,093 - MMP_RS05165 0.85 +0.8 984,093 - MMP_RS05165 0.85 +1.6 984,159 - -0.8 984,160 + -0.6
Or see this region's nucleotide sequence