Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides BWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotides BWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotides BWI76_RS13130 and BWI76_RS13135 overlap by 1 nucleotides
BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133
_RS13115
BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924
_RS13120
BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349
_RS13125
BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761
_RS13130
BWI76_RS13135: BWI76_RS13135 - 3-oxoadipyl-CoA thiolase, at 2,738,761 to 2,739,966
_RS13135
Position (kb)
2736
2737
2738
2739 Strain fitness (log2 ratio)
-1
0
1
2 at 2736.150 kb on - strand at 2736.166 kb on - strand at 2736.758 kb on + strand, within BWI76_RS13120 at 2736.963 kb on - strand at 2737.662 kb on + strand, within BWI76_RS13125 at 2737.786 kb on + strand, within BWI76_RS13125 at 2737.794 kb on - strand, within BWI76_RS13125 at 2737.877 kb on + strand, within BWI76_RS13125 at 2738.546 kb on + strand, within BWI76_RS13130 at 2738.845 kb on - strand at 2738.935 kb on - strand, within BWI76_RS13135 at 2739.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,736,150 - +0.1 2,736,166 - +0.5 2,736,758 + BWI76_RS13120 0.79 -0.2 2,736,963 - +0.1 2,737,662 + BWI76_RS13125 0.52 +0.4 2,737,786 + BWI76_RS13125 0.60 +0.2 2,737,794 - BWI76_RS13125 0.61 +0.1 2,737,877 + BWI76_RS13125 0.67 -0.3 2,738,546 + BWI76_RS13130 0.49 -0.1 2,738,845 - +0.7 2,738,935 - BWI76_RS13135 0.14 -0.3 2,739,311 - +1.9
Or see this region's nucleotide sequence