Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_587

Experiment: Gjerstad humics 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_586 and Pf6N2E2_587 are separated by 147 nucleotidesPf6N2E2_587 and Pf6N2E2_588 are separated by 65 nucleotidesPf6N2E2_588 and Pf6N2E2_589 are separated by 89 nucleotides Pf6N2E2_586 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 663,474 to 664,874 _586 Pf6N2E2_587 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 665,022 to 665,789 _587 Pf6N2E2_588 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 665,855 to 666,613 _588 Pf6N2E2_589 - Ribonuclease HI (EC 3.1.26.4), at 666,703 to 667,155 _589 Position (kb) 665 666Strain fitness (log2 ratio) -1 0 1 2 3at 664.139 kb on - strand, within Pf6N2E2_586at 664.189 kb on + strand, within Pf6N2E2_586at 664.189 kb on + strand, within Pf6N2E2_586at 664.189 kb on + strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.295 kb on - strand, within Pf6N2E2_586at 664.352 kb on - strand, within Pf6N2E2_586at 664.399 kb on + strandat 664.399 kb on + strandat 664.400 kb on - strand, within Pf6N2E2_586at 664.400 kb on - strand, within Pf6N2E2_586at 664.427 kb on - strand, within Pf6N2E2_586at 664.480 kb on + strand, within Pf6N2E2_586at 664.495 kb on + strand, within Pf6N2E2_586at 664.567 kb on + strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.576 kb on + strand, within Pf6N2E2_586at 664.576 kb on + strand, within Pf6N2E2_586at 664.933 kb on + strandat 665.004 kb on + strandat 665.023 kb on + strandat 665.023 kb on + strandat 665.024 kb on - strandat 665.030 kb on + strandat 665.030 kb on + strandat 665.031 kb on - strandat 665.031 kb on - strandat 665.282 kb on + strand, within Pf6N2E2_587at 665.282 kb on + strand, within Pf6N2E2_587at 665.282 kb on + strand, within Pf6N2E2_587at 665.282 kb on + strand, within Pf6N2E2_587at 665.283 kb on - strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.285 kb on - strand, within Pf6N2E2_587at 665.285 kb on - strand, within Pf6N2E2_587at 665.299 kb on + strand, within Pf6N2E2_587at 665.299 kb on + strand, within Pf6N2E2_587at 665.300 kb on - strand, within Pf6N2E2_587at 665.310 kb on - strand, within Pf6N2E2_587at 665.431 kb on + strand, within Pf6N2E2_587at 665.431 kb on + strand, within Pf6N2E2_587at 665.431 kb on + strand, within Pf6N2E2_587at 665.434 kb on + strand, within Pf6N2E2_587at 665.470 kb on + strand, within Pf6N2E2_587at 665.470 kb on + strand, within Pf6N2E2_587at 665.736 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.750 kb on - strandat 665.750 kb on - strandat 665.750 kb on - strandat 665.750 kb on - strandat 665.785 kb on - strandat 665.785 kb on - strandat 665.785 kb on - strandat 665.826 kb on + strandat 665.826 kb on + strandat 665.827 kb on - strandat 666.018 kb on - strand, within Pf6N2E2_588at 666.489 kb on - strand, within Pf6N2E2_588at 666.611 kb on + strandat 666.694 kb on + strandat 666.694 kb on + strandat 666.716 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gjerstad humics 0.5 M
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664,139 - Pf6N2E2_586 0.47 +1.9
664,189 + Pf6N2E2_586 0.51 +1.3
664,189 + Pf6N2E2_586 0.51 +1.0
664,189 + Pf6N2E2_586 0.51 +0.6
664,190 - Pf6N2E2_586 0.51 -0.1
664,190 - Pf6N2E2_586 0.51 +1.3
664,190 - Pf6N2E2_586 0.51 +0.1
664,190 - Pf6N2E2_586 0.51 +1.3
664,190 - Pf6N2E2_586 0.51 +1.2
664,190 - Pf6N2E2_586 0.51 -1.1
664,190 - Pf6N2E2_586 0.51 +0.5
664,295 - Pf6N2E2_586 0.59 -1.5
664,352 - Pf6N2E2_586 0.63 -0.3
664,399 + +0.8
664,399 + +0.7
664,400 - Pf6N2E2_586 0.66 +0.4
664,400 - Pf6N2E2_586 0.66 +3.3
664,427 - Pf6N2E2_586 0.68 +1.3
664,480 + Pf6N2E2_586 0.72 +0.9
664,495 + Pf6N2E2_586 0.73 +0.5
664,567 + Pf6N2E2_586 0.78 +0.6
664,568 - Pf6N2E2_586 0.78 +0.1
664,568 - Pf6N2E2_586 0.78 +1.4
664,568 - Pf6N2E2_586 0.78 -0.4
664,568 - Pf6N2E2_586 0.78 +1.6
664,576 + Pf6N2E2_586 0.79 +0.5
664,576 + Pf6N2E2_586 0.79 +1.3
664,933 + +1.0
665,004 + -1.3
665,023 + +0.5
665,023 + +0.4
665,024 - -0.6
665,030 + -1.2
665,030 + +0.7
665,031 - +0.5
665,031 - +0.9
665,282 + Pf6N2E2_587 0.34 +0.0
665,282 + Pf6N2E2_587 0.34 -0.4
665,282 + Pf6N2E2_587 0.34 -0.6
665,282 + Pf6N2E2_587 0.34 +1.7
665,283 - Pf6N2E2_587 0.34 +0.3
665,284 + Pf6N2E2_587 0.34 -1.2
665,284 + Pf6N2E2_587 0.34 -0.3
665,284 + Pf6N2E2_587 0.34 -0.9
665,284 + Pf6N2E2_587 0.34 -0.0
665,284 + Pf6N2E2_587 0.34 +0.4
665,284 + Pf6N2E2_587 0.34 +1.3
665,284 + Pf6N2E2_587 0.34 +0.5
665,284 + Pf6N2E2_587 0.34 +0.7
665,285 - Pf6N2E2_587 0.34 -0.3
665,285 - Pf6N2E2_587 0.34 -1.3
665,299 + Pf6N2E2_587 0.36 +0.0
665,299 + Pf6N2E2_587 0.36 +0.1
665,300 - Pf6N2E2_587 0.36 +0.3
665,310 - Pf6N2E2_587 0.38 -0.4
665,431 + Pf6N2E2_587 0.53 -0.6
665,431 + Pf6N2E2_587 0.53 -0.9
665,431 + Pf6N2E2_587 0.53 -1.0
665,434 + Pf6N2E2_587 0.54 -0.5
665,470 + Pf6N2E2_587 0.58 -0.7
665,470 + Pf6N2E2_587 0.58 -0.6
665,736 + -0.8
665,749 + -0.4
665,749 + -0.7
665,749 + +0.7
665,749 + -1.3
665,749 + -0.8
665,749 + -0.1
665,750 - +1.3
665,750 - +1.1
665,750 - +0.3
665,750 - +2.8
665,785 - -0.6
665,785 - -1.2
665,785 - -1.1
665,826 + +0.5
665,826 + -1.7
665,827 - -1.3
666,018 - Pf6N2E2_588 0.21 -0.7
666,489 - Pf6N2E2_588 0.84 -0.4
666,611 + +0.5
666,694 + -0.7
666,694 + +0.3
666,716 + -1.2

Or see this region's nucleotide sequence