Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4359

Experiment: L-Lysine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4357 and Pf1N1B4_4358 are separated by 405 nucleotidesPf1N1B4_4358 and Pf1N1B4_4359 are separated by 180 nucleotidesPf1N1B4_4359 and Pf1N1B4_4360 are separated by 11 nucleotidesPf1N1B4_4360 and Pf1N1B4_4361 are separated by 27 nucleotides Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943 _4357 Pf1N1B4_4358 - Acetyltransferase, GNAT family, at 4,816,349 to 4,816,711 _4358 Pf1N1B4_4359 - PvcA protein, related to known isonitrile synthases, at 4,816,892 to 4,817,887 _4359 Pf1N1B4_4360 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 4,817,899 to 4,818,603 _4360 Pf1N1B4_4361 - PvcB protein, related to amino acid oxidizing enzymes, at 4,818,631 to 4,820,391 _4361 Position (kb) 4816 4817 4818Strain fitness (log2 ratio) -1 0 1at 4816.008 kb on + strandat 4816.169 kb on + strandat 4816.169 kb on + strandat 4816.170 kb on - strandat 4816.170 kb on - strandat 4816.209 kb on - strandat 4816.209 kb on - strandat 4816.334 kb on + strandat 4816.335 kb on - strandat 4816.337 kb on - strandat 4816.386 kb on - strand, within Pf1N1B4_4358at 4816.538 kb on + strand, within Pf1N1B4_4358at 4816.648 kb on + strand, within Pf1N1B4_4358at 4816.648 kb on + strand, within Pf1N1B4_4358at 4816.652 kb on + strand, within Pf1N1B4_4358at 4816.728 kb on + strandat 4816.840 kb on + strandat 4816.892 kb on - strandat 4816.892 kb on - strandat 4817.202 kb on + strand, within Pf1N1B4_4359at 4817.202 kb on + strand, within Pf1N1B4_4359at 4817.203 kb on - strand, within Pf1N1B4_4359at 4817.203 kb on - strandat 4817.203 kb on - strandat 4817.234 kb on + strand, within Pf1N1B4_4359at 4817.234 kb on + strand, within Pf1N1B4_4359at 4817.234 kb on + strand, within Pf1N1B4_4359at 4817.235 kb on - strand, within Pf1N1B4_4359at 4817.273 kb on + strand, within Pf1N1B4_4359at 4817.274 kb on - strandat 4817.321 kb on + strand, within Pf1N1B4_4359at 4817.510 kb on + strand, within Pf1N1B4_4359at 4817.544 kb on - strand, within Pf1N1B4_4359at 4817.559 kb on + strand, within Pf1N1B4_4359at 4817.871 kb on - strandat 4817.915 kb on + strandat 4818.031 kb on + strand, within Pf1N1B4_4360at 4818.115 kb on + strand, within Pf1N1B4_4360at 4818.439 kb on + strand, within Pf1N1B4_4360at 4818.591 kb on + strandat 4818.598 kb on + strandat 4818.599 kb on - strandat 4818.599 kb on - strandat 4818.605 kb on - strandat 4818.639 kb on + strandat 4818.640 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine (N)
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4,816,008 + +1.4
4,816,169 + +0.2
4,816,169 + -0.2
4,816,170 - -0.1
4,816,170 - +0.5
4,816,209 - -0.0
4,816,209 - +0.0
4,816,334 + -1.3
4,816,335 - -0.5
4,816,337 - +0.7
4,816,386 - Pf1N1B4_4358 0.10 +0.7
4,816,538 + Pf1N1B4_4358 0.52 +1.0
4,816,648 + Pf1N1B4_4358 0.82 -0.3
4,816,648 + Pf1N1B4_4358 0.82 +0.3
4,816,652 + Pf1N1B4_4358 0.83 -0.3
4,816,728 + -0.6
4,816,840 + +1.5
4,816,892 - -0.3
4,816,892 - -0.6
4,817,202 + Pf1N1B4_4359 0.31 -0.6
4,817,202 + Pf1N1B4_4359 0.31 +0.1
4,817,203 - Pf1N1B4_4359 0.31 -0.1
4,817,203 - -0.4
4,817,203 - -0.9
4,817,234 + Pf1N1B4_4359 0.34 -1.2
4,817,234 + Pf1N1B4_4359 0.34 +0.7
4,817,234 + Pf1N1B4_4359 0.34 +0.2
4,817,235 - Pf1N1B4_4359 0.34 +0.3
4,817,273 + Pf1N1B4_4359 0.38 -0.0
4,817,274 - +0.1
4,817,321 + Pf1N1B4_4359 0.43 +0.4
4,817,510 + Pf1N1B4_4359 0.62 -0.4
4,817,544 - Pf1N1B4_4359 0.65 -0.2
4,817,559 + Pf1N1B4_4359 0.67 +0.3
4,817,871 - -0.5
4,817,915 + -0.9
4,818,031 + Pf1N1B4_4360 0.19 -0.5
4,818,115 + Pf1N1B4_4360 0.31 -0.4
4,818,439 + Pf1N1B4_4360 0.77 +1.4
4,818,591 + -0.6
4,818,598 + -1.3
4,818,599 - -0.6
4,818,599 - -0.1
4,818,605 - +0.7
4,818,639 + -0.7
4,818,640 - +0.3

Or see this region's nucleotide sequence