Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_223 and Pf1N1B4_224 overlap by 4 nucleotides Pf1N1B4_224 and Pf1N1B4_225 are separated by 116 nucleotides Pf1N1B4_225 and Pf1N1B4_226 are separated by 71 nucleotides Pf1N1B4_226 and Pf1N1B4_227 are separated by 74 nucleotides
Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912
_223
Pf1N1B4_224 - Isohexenylglutaconyl-CoA hydratase, at 284,909 to 285,706
_224
Pf1N1B4_225 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 285,823 to 287,784
_225
Pf1N1B4_226 - Inhibitor of the KinA pathway to sporulation, predicted exonuclease, at 287,856 to 288,404
_226
Pf1N1B4_227 - FIG074102: hypothetical protein, at 288,479 to 288,763
_227
Position (kb) 285
286
287
288 Strain fitness (log2 ratio) -1
0
1
2 at 285.288 kb on + strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.806 kb on + strand at 285.806 kb on + strand at 285.806 kb on + strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.920 kb on + strand at 286.006 kb on + strand at 286.007 kb on - strand at 286.007 kb on - strand at 286.495 kb on + strand, within Pf1N1B4_225 at 286.495 kb on + strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225 at 286.846 kb on + strand, within Pf1N1B4_225 at 286.972 kb on + strand, within Pf1N1B4_225 at 286.972 kb on + strand, within Pf1N1B4_225 at 286.973 kb on - strand, within Pf1N1B4_225 at 287.234 kb on - strand, within Pf1N1B4_225 at 287.312 kb on + strand, within Pf1N1B4_225 at 287.391 kb on + strand, within Pf1N1B4_225 at 287.615 kb on + strand at 287.615 kb on + strand at 287.616 kb on - strand at 287.654 kb on + strand at 287.719 kb on - strand at 287.719 kb on - strand at 287.783 kb on - strand at 288.163 kb on - strand, within Pf1N1B4_226 at 288.362 kb on + strand at 288.363 kb on - strand at 288.440 kb on - strand at 288.484 kb on + strand at 288.484 kb on + strand at 288.498 kb on - strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.741 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 285,288 + Pf1N1B4_224 0.47 -1.6 285,289 - Pf1N1B4_224 0.48 +0.1 285,289 - Pf1N1B4_224 0.48 +1.5 285,289 - Pf1N1B4_224 0.48 -0.1 285,806 + +1.4 285,806 + -0.5 285,806 + -0.4 285,807 - -0.5 285,807 - +0.0 285,807 - -0.9 285,807 - -1.2 285,807 - +0.4 285,807 - -0.5 285,920 + +0.7 286,006 + -0.4 286,007 - -0.1 286,007 - +0.1 286,495 + Pf1N1B4_225 0.34 -0.9 286,495 + Pf1N1B4_225 0.34 -0.7 286,631 - Pf1N1B4_225 0.41 -0.9 286,631 - Pf1N1B4_225 0.41 -1.5 286,631 - Pf1N1B4_225 0.41 +0.2 286,631 - Pf1N1B4_225 0.41 -0.4 286,682 - Pf1N1B4_225 0.44 -1.5 286,682 - Pf1N1B4_225 0.44 -1.1 286,846 + Pf1N1B4_225 0.52 -0.9 286,972 + Pf1N1B4_225 0.59 -1.3 286,972 + Pf1N1B4_225 0.59 +0.8 286,973 - Pf1N1B4_225 0.59 -0.3 287,234 - Pf1N1B4_225 0.72 +0.8 287,312 + Pf1N1B4_225 0.76 -0.3 287,391 + Pf1N1B4_225 0.80 -0.2 287,615 + -0.2 287,615 + -1.4 287,616 - +0.2 287,654 + +0.1 287,719 - +0.5 287,719 - +2.1 287,783 - -0.4 288,163 - Pf1N1B4_226 0.56 -0.8 288,362 + +0.5 288,363 - +0.6 288,440 - +0.2 288,484 + +1.2 288,484 + +0.2 288,498 - -0.5 288,740 + +0.2 288,740 + -1.1 288,740 + -0.4 288,740 + +1.4 288,741 - +0.6
Or see this region's nucleotide sequence