Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27165

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27160 and BWI76_RS27165 are separated by 127 nucleotidesBWI76_RS27165 and BWI76_RS27170 overlap by 4 nucleotidesBWI76_RS27170 and BWI76_RS27175 overlap by 12 nucleotides BWI76_RS27160: BWI76_RS27160 - PTS mannitol transporter subunit IICBA, at 5,638,012 to 5,639,922 _RS27160 BWI76_RS27165: BWI76_RS27165 - mannitol-1-phosphate 5-dehydrogenase, at 5,640,050 to 5,641,198 _RS27165 BWI76_RS27170: BWI76_RS27170 - MltR family transcriptional regulator, at 5,641,195 to 5,641,791 _RS27170 BWI76_RS27175: BWI76_RS27175 - hypothetical protein, at 5,641,780 to 5,642,004 _RS27175 Position (kb) 5640 5641 5642Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5639.292 kb on + strand, within BWI76_RS27160at 5639.421 kb on + strand, within BWI76_RS27160at 5639.483 kb on + strand, within BWI76_RS27160at 5639.491 kb on - strand, within BWI76_RS27160at 5639.585 kb on - strand, within BWI76_RS27160at 5639.617 kb on + strandat 5639.625 kb on - strand, within BWI76_RS27160at 5639.661 kb on - strand, within BWI76_RS27160at 5639.666 kb on - strand, within BWI76_RS27160at 5639.899 kb on + strandat 5639.899 kb on + strandat 5639.958 kb on + strandat 5639.978 kb on - strandat 5639.983 kb on + strandat 5639.984 kb on + strandat 5639.990 kb on - strandat 5639.994 kb on + strandat 5640.014 kb on - strandat 5640.225 kb on - strand, within BWI76_RS27165at 5640.335 kb on + strand, within BWI76_RS27165at 5640.444 kb on - strand, within BWI76_RS27165at 5640.523 kb on - strand, within BWI76_RS27165at 5640.613 kb on + strand, within BWI76_RS27165at 5640.621 kb on - strand, within BWI76_RS27165at 5640.621 kb on - strand, within BWI76_RS27165at 5640.621 kb on - strand, within BWI76_RS27165at 5640.686 kb on + strand, within BWI76_RS27165at 5640.990 kb on - strand, within BWI76_RS27165at 5640.996 kb on + strand, within BWI76_RS27165at 5641.215 kb on + strandat 5641.434 kb on + strand, within BWI76_RS27170at 5641.487 kb on + strand, within BWI76_RS27170at 5641.495 kb on - strand, within BWI76_RS27170at 5641.538 kb on - strand, within BWI76_RS27170at 5641.549 kb on + strand, within BWI76_RS27170at 5641.567 kb on + strand, within BWI76_RS27170at 5641.570 kb on + strand, within BWI76_RS27170at 5641.664 kb on + strand, within BWI76_RS27170at 5641.705 kb on + strand, within BWI76_RS27170at 5641.736 kb on - strandat 5641.736 kb on - strandat 5641.798 kb on - strandat 5641.839 kb on + strand, within BWI76_RS27175at 5641.840 kb on - strand, within BWI76_RS27175at 5641.889 kb on + strand, within BWI76_RS27175at 5641.941 kb on - strand, within BWI76_RS27175at 5642.008 kb on + strandat 5642.008 kb on + strandat 5642.011 kb on + strandat 5642.011 kb on + strandat 5642.013 kb on + strandat 5642.016 kb on - strandat 5642.016 kb on - strandat 5642.018 kb on + strandat 5642.019 kb on - strandat 5642.019 kb on - strandat 5642.019 kb on - strandat 5642.023 kb on + strandat 5642.031 kb on - strandat 5642.039 kb on + strandat 5642.085 kb on + strandat 5642.159 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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5,639,292 + BWI76_RS27160 0.67 -0.5
5,639,421 + BWI76_RS27160 0.74 +0.9
5,639,483 + BWI76_RS27160 0.77 +0.4
5,639,491 - BWI76_RS27160 0.77 +0.4
5,639,585 - BWI76_RS27160 0.82 -1.1
5,639,617 + +2.7
5,639,625 - BWI76_RS27160 0.84 +1.7
5,639,661 - BWI76_RS27160 0.86 +0.4
5,639,666 - BWI76_RS27160 0.87 +0.2
5,639,899 + -0.2
5,639,899 + +0.4
5,639,958 + +1.7
5,639,978 - +0.1
5,639,983 + +0.3
5,639,984 + -1.5
5,639,990 - -0.4
5,639,994 + -0.5
5,640,014 - +1.7
5,640,225 - BWI76_RS27165 0.15 -1.3
5,640,335 + BWI76_RS27165 0.25 -1.4
5,640,444 - BWI76_RS27165 0.34 -0.1
5,640,523 - BWI76_RS27165 0.41 -1.1
5,640,613 + BWI76_RS27165 0.49 +1.3
5,640,621 - BWI76_RS27165 0.50 -1.4
5,640,621 - BWI76_RS27165 0.50 -0.8
5,640,621 - BWI76_RS27165 0.50 -1.4
5,640,686 + BWI76_RS27165 0.55 +1.7
5,640,990 - BWI76_RS27165 0.82 -2.1
5,640,996 + BWI76_RS27165 0.82 +0.4
5,641,215 + -0.4
5,641,434 + BWI76_RS27170 0.40 -3.2
5,641,487 + BWI76_RS27170 0.49 -1.3
5,641,495 - BWI76_RS27170 0.50 -3.7
5,641,538 - BWI76_RS27170 0.57 -1.8
5,641,549 + BWI76_RS27170 0.59 +0.7
5,641,567 + BWI76_RS27170 0.62 -1.7
5,641,570 + BWI76_RS27170 0.63 -1.3
5,641,664 + BWI76_RS27170 0.79 -2.4
5,641,705 + BWI76_RS27170 0.85 -1.3
5,641,736 - -1.6
5,641,736 - -1.8
5,641,798 - +0.7
5,641,839 + BWI76_RS27175 0.26 -1.3
5,641,840 - BWI76_RS27175 0.27 -0.3
5,641,889 + BWI76_RS27175 0.48 -0.5
5,641,941 - BWI76_RS27175 0.72 -0.5
5,642,008 + +0.0
5,642,008 + +1.0
5,642,011 + -0.1
5,642,011 + -0.3
5,642,013 + -0.4
5,642,016 - +0.5
5,642,016 - +1.3
5,642,018 + +0.5
5,642,019 - +0.3
5,642,019 - -0.6
5,642,019 - -0.3
5,642,023 + +0.1
5,642,031 - +0.8
5,642,039 + +0.9
5,642,085 + +0.1
5,642,159 - +0.6

Or see this region's nucleotide sequence