Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22370

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22365 and BWI76_RS22370 are separated by 258 nucleotidesBWI76_RS22370 and BWI76_RS22375 are separated by 117 nucleotides BWI76_RS22365: BWI76_RS22365 - hydrogenase expression/formation protein HypE, at 4,660,584 to 4,661,594 _RS22365 BWI76_RS22370: BWI76_RS22370 - transcriptional regulator FhlA, at 4,661,853 to 4,663,925 _RS22370 BWI76_RS22375: BWI76_RS22375 - pectin acetylesterase, at 4,664,043 to 4,664,990 _RS22375 Position (kb) 4661 4662 4663 4664Strain fitness (log2 ratio) -1 0 1 2 3at 4661.187 kb on + strand, within BWI76_RS22365at 4661.235 kb on + strand, within BWI76_RS22365at 4661.416 kb on - strand, within BWI76_RS22365at 4661.524 kb on + strandat 4661.639 kb on + strandat 4661.653 kb on - strandat 4662.124 kb on - strand, within BWI76_RS22370at 4662.147 kb on - strand, within BWI76_RS22370at 4662.375 kb on - strand, within BWI76_RS22370at 4662.433 kb on + strand, within BWI76_RS22370at 4662.439 kb on - strand, within BWI76_RS22370at 4662.523 kb on + strand, within BWI76_RS22370at 4662.531 kb on - strand, within BWI76_RS22370at 4662.531 kb on - strand, within BWI76_RS22370at 4662.560 kb on + strand, within BWI76_RS22370at 4662.693 kb on - strand, within BWI76_RS22370at 4662.770 kb on - strand, within BWI76_RS22370at 4662.783 kb on - strand, within BWI76_RS22370at 4662.965 kb on + strand, within BWI76_RS22370at 4663.034 kb on + strand, within BWI76_RS22370at 4663.065 kb on + strand, within BWI76_RS22370at 4663.065 kb on + strand, within BWI76_RS22370at 4663.755 kb on - strandat 4664.374 kb on - strand, within BWI76_RS22375at 4664.410 kb on + strand, within BWI76_RS22375at 4664.418 kb on - strand, within BWI76_RS22375at 4664.420 kb on + strand, within BWI76_RS22375at 4664.641 kb on - strand, within BWI76_RS22375at 4664.752 kb on - strand, within BWI76_RS22375at 4664.921 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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4,661,187 + BWI76_RS22365 0.60 -0.2
4,661,235 + BWI76_RS22365 0.64 -0.2
4,661,416 - BWI76_RS22365 0.82 -0.2
4,661,524 + -0.9
4,661,639 + +0.3
4,661,653 - -0.7
4,662,124 - BWI76_RS22370 0.13 -0.3
4,662,147 - BWI76_RS22370 0.14 +0.3
4,662,375 - BWI76_RS22370 0.25 +0.4
4,662,433 + BWI76_RS22370 0.28 +0.7
4,662,439 - BWI76_RS22370 0.28 -0.3
4,662,523 + BWI76_RS22370 0.32 +0.1
4,662,531 - BWI76_RS22370 0.33 -0.2
4,662,531 - BWI76_RS22370 0.33 +0.1
4,662,560 + BWI76_RS22370 0.34 -0.4
4,662,693 - BWI76_RS22370 0.41 +0.8
4,662,770 - BWI76_RS22370 0.44 -0.5
4,662,783 - BWI76_RS22370 0.45 +0.4
4,662,965 + BWI76_RS22370 0.54 -0.3
4,663,034 + BWI76_RS22370 0.57 -0.1
4,663,065 + BWI76_RS22370 0.58 -0.2
4,663,065 + BWI76_RS22370 0.58 -0.2
4,663,755 - -0.2
4,664,374 - BWI76_RS22375 0.35 -1.2
4,664,410 + BWI76_RS22375 0.39 +1.1
4,664,418 - BWI76_RS22375 0.40 +3.4
4,664,420 + BWI76_RS22375 0.40 -0.8
4,664,641 - BWI76_RS22375 0.63 +0.4
4,664,752 - BWI76_RS22375 0.75 +0.9
4,664,921 - -0.8

Or see this region's nucleotide sequence