Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22330

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22320 and BWI76_RS22325 are separated by 17 nucleotidesBWI76_RS22325 and BWI76_RS22330 are separated by 2 nucleotidesBWI76_RS22330 and BWI76_RS22335 overlap by 4 nucleotidesBWI76_RS22335 and BWI76_RS22340 are separated by 82 nucleotides BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860 _RS22320 BWI76_RS22325: BWI76_RS22325 - hydrogenase 3 membrane subunit, at 4,653,878 to 4,654,801 _RS22325 BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630 _RS22330 BWI76_RS22335: BWI76_RS22335 - 4Fe-4S ferredoxin, at 4,656,627 to 4,657,235 _RS22335 BWI76_RS22340: BWI76_RS22340 - formate hydrogenlyase regulatory protein HycA, at 4,657,318 to 4,657,767 _RS22340 Position (kb) 4654 4655 4656 4657Strain fitness (log2 ratio) -1 0 1at 4653.956 kb on - strandat 4654.110 kb on + strand, within BWI76_RS22325at 4654.128 kb on - strand, within BWI76_RS22325at 4654.312 kb on - strand, within BWI76_RS22325at 4654.328 kb on + strand, within BWI76_RS22325at 4654.367 kb on - strand, within BWI76_RS22325at 4654.576 kb on + strand, within BWI76_RS22325at 4654.862 kb on + strandat 4655.163 kb on - strand, within BWI76_RS22330at 4655.294 kb on - strand, within BWI76_RS22330at 4655.321 kb on - strand, within BWI76_RS22330at 4655.682 kb on - strand, within BWI76_RS22330at 4655.909 kb on + strand, within BWI76_RS22330at 4656.349 kb on - strand, within BWI76_RS22330at 4656.400 kb on + strand, within BWI76_RS22330at 4656.438 kb on + strand, within BWI76_RS22330at 4656.474 kb on - strandat 4656.770 kb on - strand, within BWI76_RS22335at 4656.836 kb on + strand, within BWI76_RS22335at 4656.849 kb on - strand, within BWI76_RS22335at 4656.883 kb on + strand, within BWI76_RS22335at 4656.921 kb on - strand, within BWI76_RS22335at 4657.292 kb on - strandat 4657.609 kb on - strand, within BWI76_RS22340

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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4,653,956 - -0.2
4,654,110 + BWI76_RS22325 0.25 -0.1
4,654,128 - BWI76_RS22325 0.27 +0.6
4,654,312 - BWI76_RS22325 0.47 +0.2
4,654,328 + BWI76_RS22325 0.49 +1.5
4,654,367 - BWI76_RS22325 0.53 +0.1
4,654,576 + BWI76_RS22325 0.76 +0.1
4,654,862 + -0.5
4,655,163 - BWI76_RS22330 0.20 -0.4
4,655,294 - BWI76_RS22330 0.27 -0.2
4,655,321 - BWI76_RS22330 0.28 +0.1
4,655,682 - BWI76_RS22330 0.48 +0.6
4,655,909 + BWI76_RS22330 0.60 +0.1
4,656,349 - BWI76_RS22330 0.85 -0.8
4,656,400 + BWI76_RS22330 0.87 -1.0
4,656,438 + BWI76_RS22330 0.89 -0.5
4,656,474 - -1.5
4,656,770 - BWI76_RS22335 0.23 -0.1
4,656,836 + BWI76_RS22335 0.34 -0.2
4,656,849 - BWI76_RS22335 0.36 -0.2
4,656,883 + BWI76_RS22335 0.42 -0.3
4,656,921 - BWI76_RS22335 0.48 -0.1
4,657,292 - -1.0
4,657,609 - BWI76_RS22340 0.65 +0.3

Or see this region's nucleotide sequence