Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12500 and BWI76_RS12505 overlap by 10 nucleotides BWI76_RS12505 and BWI76_RS12510 are separated by 188 nucleotides BWI76_RS12510 and BWI76_RS12515 are separated by 92 nucleotides BWI76_RS12515 and BWI76_RS12520 are separated by 257 nucleotides
BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694
_RS12500
BWI76_RS12505: BWI76_RS12505 - murein peptide amidase A, at 2,611,685 to 2,612,398
_RS12505
BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203
_RS12510
BWI76_RS12515: BWI76_RS12515 - protease, at 2,614,296 to 2,614,859
_RS12515
BWI76_RS12520: BWI76_RS12520 - hypothetical protein, at 2,615,117 to 2,615,320
_RS12520
Position (kb)
2612
2613
2614
2615 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2611.695 kb on - strand at 2612.394 kb on + strand at 2612.401 kb on - strand at 2612.484 kb on + strand at 2612.484 kb on + strand at 2612.592 kb on - strand at 2612.717 kb on - strand at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.881 kb on - strand, within BWI76_RS12510 at 2613.077 kb on + strand, within BWI76_RS12510 at 2613.281 kb on + strand, within BWI76_RS12510 at 2613.317 kb on + strand, within BWI76_RS12510 at 2613.320 kb on - strand, within BWI76_RS12510 at 2613.438 kb on + strand, within BWI76_RS12510 at 2613.438 kb on + strand, within BWI76_RS12510 at 2613.438 kb on + strand, within BWI76_RS12510 at 2613.446 kb on - strand, within BWI76_RS12510 at 2613.446 kb on - strand, within BWI76_RS12510 at 2613.518 kb on + strand, within BWI76_RS12510 at 2613.749 kb on + strand, within BWI76_RS12510 at 2613.941 kb on + strand, within BWI76_RS12510 at 2613.949 kb on - strand, within BWI76_RS12510 at 2614.102 kb on - strand at 2614.168 kb on + strand at 2614.625 kb on - strand, within BWI76_RS12515 at 2614.653 kb on + strand, within BWI76_RS12515 at 2614.889 kb on + strand at 2614.901 kb on - strand at 2614.901 kb on - strand at 2614.963 kb on + strand at 2615.139 kb on + strand, within BWI76_RS12520
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 2,611,695 - +0.1 2,612,394 + +0.9 2,612,401 - -0.6 2,612,484 + +0.5 2,612,484 + -0.3 2,612,592 - -0.3 2,612,717 - +1.0 2,612,873 + BWI76_RS12510 0.18 +0.3 2,612,873 + BWI76_RS12510 0.18 +1.2 2,612,873 + BWI76_RS12510 0.18 +0.6 2,612,873 + BWI76_RS12510 0.18 +0.3 2,612,881 - BWI76_RS12510 0.18 +0.5 2,613,077 + BWI76_RS12510 0.30 +0.7 2,613,281 + BWI76_RS12510 0.43 +0.3 2,613,317 + BWI76_RS12510 0.45 +0.9 2,613,320 - BWI76_RS12510 0.45 +0.0 2,613,438 + BWI76_RS12510 0.53 +0.4 2,613,438 + BWI76_RS12510 0.53 +0.7 2,613,438 + BWI76_RS12510 0.53 +1.1 2,613,446 - BWI76_RS12510 0.53 +0.2 2,613,446 - BWI76_RS12510 0.53 +1.2 2,613,518 + BWI76_RS12510 0.58 +0.3 2,613,749 + BWI76_RS12510 0.72 +0.9 2,613,941 + BWI76_RS12510 0.84 -0.9 2,613,949 - BWI76_RS12510 0.84 +0.4 2,614,102 - +0.5 2,614,168 + +1.8 2,614,625 - BWI76_RS12515 0.58 -0.2 2,614,653 + BWI76_RS12515 0.63 -0.2 2,614,889 + -0.4 2,614,901 - -0.4 2,614,901 - +0.1 2,614,963 + -2.0 2,615,139 + BWI76_RS12520 0.11 -0.4
Or see this region's nucleotide sequence