Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06490

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06475 and BWI76_RS06480 are separated by 11 nucleotidesBWI76_RS06480 and BWI76_RS06485 overlap by 1 nucleotidesBWI76_RS06485 and BWI76_RS06490 overlap by 11 nucleotidesBWI76_RS06490 and BWI76_RS06495 are separated by 19 nucleotides BWI76_RS06475: BWI76_RS06475 - protoheme IX farnesyltransferase, at 1,360,537 to 1,361,424 _RS06475 BWI76_RS06480: BWI76_RS06480 - cytochrome o ubiquinol oxidase subunit IV, at 1,361,436 to 1,361,765 _RS06480 BWI76_RS06485: BWI76_RS06485 - cytochrome o ubiquinol oxidase subunit III, at 1,361,765 to 1,362,376 _RS06485 BWI76_RS06490: BWI76_RS06490 - cytochrome ubiquinol oxidase subunit I, at 1,362,366 to 1,364,357 _RS06490 BWI76_RS06495: BWI76_RS06495 - cytochrome ubiquinol oxidase subunit II, at 1,364,377 to 1,365,324 _RS06495 Position (kb) 1362 1363 1364 1365Strain fitness (log2 ratio) -3 -2 -1 0 1at 1361.591 kb on - strand, within BWI76_RS06480at 1361.856 kb on - strand, within BWI76_RS06485at 1361.946 kb on + strand, within BWI76_RS06485at 1362.441 kb on - strandat 1362.686 kb on - strand, within BWI76_RS06490at 1363.047 kb on + strand, within BWI76_RS06490at 1363.073 kb on - strand, within BWI76_RS06490at 1363.232 kb on - strand, within BWI76_RS06490at 1363.387 kb on + strand, within BWI76_RS06490at 1363.759 kb on - strandat 1364.100 kb on + strand, within BWI76_RS06490at 1364.131 kb on - strand, within BWI76_RS06490at 1364.398 kb on + strandat 1364.460 kb on - strandat 1364.469 kb on - strandat 1364.831 kb on + strandat 1364.979 kb on + strand, within BWI76_RS06495at 1364.979 kb on + strand, within BWI76_RS06495at 1365.012 kb on - strand, within BWI76_RS06495at 1365.036 kb on - strand, within BWI76_RS06495at 1365.057 kb on - strand, within BWI76_RS06495at 1365.072 kb on + strand, within BWI76_RS06495at 1365.080 kb on - strand, within BWI76_RS06495at 1365.086 kb on + strand, within BWI76_RS06495at 1365.090 kb on - strand, within BWI76_RS06495at 1365.090 kb on - strand, within BWI76_RS06495at 1365.109 kb on - strand, within BWI76_RS06495at 1365.148 kb on - strand, within BWI76_RS06495at 1365.153 kb on - strand, within BWI76_RS06495at 1365.175 kb on - strand, within BWI76_RS06495at 1365.177 kb on + strand, within BWI76_RS06495at 1365.200 kb on - strand, within BWI76_RS06495at 1365.210 kb on - strand, within BWI76_RS06495at 1365.212 kb on + strand, within BWI76_RS06495at 1365.220 kb on - strand, within BWI76_RS06495at 1365.230 kb on + strandat 1365.232 kb on - strandat 1365.233 kb on - strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.259 kb on + strandat 1365.261 kb on + strandat 1365.261 kb on + strandat 1365.261 kb on + strandat 1365.262 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.267 kb on - strandat 1365.269 kb on - strandat 1365.269 kb on - strandat 1365.269 kb on - strandat 1365.269 kb on - strandat 1365.269 kb on - strandat 1365.269 kb on - strandat 1365.356 kb on + strandat 1365.356 kb on + strandat 1365.356 kb on + strandat 1365.356 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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1,361,591 - BWI76_RS06480 0.47 -2.0
1,361,856 - BWI76_RS06485 0.15 -1.1
1,361,946 + BWI76_RS06485 0.30 -1.7
1,362,441 - +0.0
1,362,686 - BWI76_RS06490 0.16 -1.1
1,363,047 + BWI76_RS06490 0.34 -0.5
1,363,073 - BWI76_RS06490 0.35 -2.0
1,363,232 - BWI76_RS06490 0.43 +0.6
1,363,387 + BWI76_RS06490 0.51 +1.1
1,363,759 - -0.3
1,364,100 + BWI76_RS06490 0.87 -1.8
1,364,131 - BWI76_RS06490 0.89 -1.2
1,364,398 + -1.4
1,364,460 - +0.1
1,364,469 - +0.3
1,364,831 + +0.1
1,364,979 + BWI76_RS06495 0.64 -2.4
1,364,979 + BWI76_RS06495 0.64 -0.9
1,365,012 - BWI76_RS06495 0.67 -2.2
1,365,036 - BWI76_RS06495 0.70 -1.5
1,365,057 - BWI76_RS06495 0.72 +0.1
1,365,072 + BWI76_RS06495 0.73 +0.3
1,365,080 - BWI76_RS06495 0.74 -2.3
1,365,086 + BWI76_RS06495 0.75 -1.8
1,365,090 - BWI76_RS06495 0.75 -2.1
1,365,090 - BWI76_RS06495 0.75 -1.4
1,365,109 - BWI76_RS06495 0.77 -0.3
1,365,148 - BWI76_RS06495 0.81 -1.8
1,365,153 - BWI76_RS06495 0.82 -1.9
1,365,175 - BWI76_RS06495 0.84 -1.5
1,365,177 + BWI76_RS06495 0.84 -1.7
1,365,200 - BWI76_RS06495 0.87 -0.8
1,365,210 - BWI76_RS06495 0.88 -2.2
1,365,212 + BWI76_RS06495 0.88 -0.9
1,365,220 - BWI76_RS06495 0.89 -1.2
1,365,230 + -1.1
1,365,232 - -1.6
1,365,233 - -1.5
1,365,259 + +0.2
1,365,259 + -1.4
1,365,259 + -1.0
1,365,259 + +0.2
1,365,259 + -0.9
1,365,259 + -0.1
1,365,259 + -0.9
1,365,259 + +0.9
1,365,259 + -1.3
1,365,261 + -1.4
1,365,261 + +1.7
1,365,261 + +0.1
1,365,262 - -2.0
1,365,267 - -1.6
1,365,267 - -1.6
1,365,267 - -2.1
1,365,267 - -1.2
1,365,267 - -1.0
1,365,267 - +0.4
1,365,267 - -1.0
1,365,267 - -0.8
1,365,267 - +0.4
1,365,267 - -1.6
1,365,267 - -1.8
1,365,267 - -1.4
1,365,267 - +0.7
1,365,267 - -3.4
1,365,267 - -1.8
1,365,267 - -0.3
1,365,269 - -2.3
1,365,269 - -1.1
1,365,269 - -1.9
1,365,269 - -0.2
1,365,269 - -2.5
1,365,269 - +0.9
1,365,356 + -2.1
1,365,356 + -2.1
1,365,356 + -0.3
1,365,356 + -3.1

Or see this region's nucleotide sequence