Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_839

Experiment: pyrophosphate 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_838 and Pf6N2E2_839 are separated by 123 nucleotidesPf6N2E2_839 and Pf6N2E2_840 are separated by 574 nucleotides Pf6N2E2_838 - Type I secretion outer membrane protein, TolC precursor, at 980,708 to 982,015 _838 Pf6N2E2_839 - Alkaline phosphatase (EC 3.1.3.1), at 982,139 to 984,760 _839 Pf6N2E2_840 - Alcohol dehydrogenase (EC 1.1.1.1), at 985,335 to 986,321 _840 Position (kb) 982 983 984 985Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 981.408 kb on - strand, within Pf6N2E2_838at 981.623 kb on + strand, within Pf6N2E2_838at 981.738 kb on - strand, within Pf6N2E2_838at 981.738 kb on - strand, within Pf6N2E2_838at 981.738 kb on - strand, within Pf6N2E2_838at 981.738 kb on - strand, within Pf6N2E2_838at 981.738 kb on - strand, within Pf6N2E2_838at 981.861 kb on + strand, within Pf6N2E2_838at 981.861 kb on + strand, within Pf6N2E2_838at 981.861 kb on + strand, within Pf6N2E2_838at 981.871 kb on + strand, within Pf6N2E2_838at 981.871 kb on + strand, within Pf6N2E2_838at 981.871 kb on + strand, within Pf6N2E2_838at 981.871 kb on + strand, within Pf6N2E2_838at 981.917 kb on + strandat 981.917 kb on + strandat 981.918 kb on - strandat 982.013 kb on + strandat 982.013 kb on + strandat 982.013 kb on + strandat 982.014 kb on - strandat 982.048 kb on + strandat 982.048 kb on + strandat 982.048 kb on + strandat 982.091 kb on + strandat 982.139 kb on - strandat 982.158 kb on - strandat 982.163 kb on + strandat 982.223 kb on + strandat 982.224 kb on - strandat 982.224 kb on - strandat 982.224 kb on - strandat 982.252 kb on + strandat 982.274 kb on + strandat 982.275 kb on - strandat 982.313 kb on + strandat 982.313 kb on + strandat 982.313 kb on + strandat 982.331 kb on + strandat 982.332 kb on - strandat 982.332 kb on - strandat 982.332 kb on - strandat 982.343 kb on + strandat 982.343 kb on + strandat 982.343 kb on + strandat 982.343 kb on + strandat 982.344 kb on - strandat 982.344 kb on - strandat 982.375 kb on + strandat 982.375 kb on + strandat 982.480 kb on + strand, within Pf6N2E2_839at 982.480 kb on + strand, within Pf6N2E2_839at 982.589 kb on + strand, within Pf6N2E2_839at 982.590 kb on - strand, within Pf6N2E2_839at 982.595 kb on + strand, within Pf6N2E2_839at 982.595 kb on + strand, within Pf6N2E2_839at 982.705 kb on + strand, within Pf6N2E2_839at 982.706 kb on - strand, within Pf6N2E2_839at 982.718 kb on + strand, within Pf6N2E2_839at 982.719 kb on - strand, within Pf6N2E2_839at 982.730 kb on + strand, within Pf6N2E2_839at 982.730 kb on + strand, within Pf6N2E2_839at 982.730 kb on + strand, within Pf6N2E2_839at 982.731 kb on - strand, within Pf6N2E2_839at 982.731 kb on - strand, within Pf6N2E2_839at 982.736 kb on + strand, within Pf6N2E2_839at 982.826 kb on + strand, within Pf6N2E2_839at 983.015 kb on + strand, within Pf6N2E2_839at 983.016 kb on - strand, within Pf6N2E2_839at 983.114 kb on - strand, within Pf6N2E2_839at 983.114 kb on - strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.144 kb on + strand, within Pf6N2E2_839at 983.145 kb on - strand, within Pf6N2E2_839at 983.145 kb on - strand, within Pf6N2E2_839at 983.145 kb on - strand, within Pf6N2E2_839at 983.228 kb on + strand, within Pf6N2E2_839at 983.336 kb on - strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.393 kb on + strand, within Pf6N2E2_839at 983.394 kb on - strand, within Pf6N2E2_839at 983.527 kb on + strand, within Pf6N2E2_839at 983.570 kb on - strand, within Pf6N2E2_839at 983.642 kb on + strand, within Pf6N2E2_839at 983.642 kb on + strand, within Pf6N2E2_839at 983.642 kb on + strand, within Pf6N2E2_839at 983.642 kb on + strand, within Pf6N2E2_839at 983.643 kb on - strandat 983.643 kb on - strand, within Pf6N2E2_839at 983.643 kb on - strand, within Pf6N2E2_839at 983.665 kb on + strand, within Pf6N2E2_839at 983.666 kb on - strand, within Pf6N2E2_839at 983.666 kb on - strand, within Pf6N2E2_839at 984.085 kb on - strand, within Pf6N2E2_839at 984.085 kb on - strand, within Pf6N2E2_839at 984.085 kb on - strand, within Pf6N2E2_839at 984.085 kb on - strand, within Pf6N2E2_839at 984.478 kb on + strand, within Pf6N2E2_839at 984.482 kb on - strand, within Pf6N2E2_839at 984.615 kb on + strandat 984.671 kb on + strandat 984.671 kb on + strandat 984.671 kb on + strandat 984.671 kb on + strandat 984.736 kb on + strandat 984.736 kb on + strandat 984.934 kb on + strandat 984.934 kb on + strandat 984.935 kb on - strandat 984.935 kb on - strandat 984.935 kb on - strandat 984.938 kb on + strandat 984.981 kb on + strandat 985.006 kb on + strandat 985.007 kb on - strandat 985.007 kb on - strandat 985.007 kb on - strandat 985.007 kb on - strandat 985.018 kb on + strandat 985.018 kb on + strandat 985.115 kb on - strandat 985.116 kb on + strandat 985.117 kb on - strandat 985.117 kb on - strandat 985.158 kb on + strandat 985.171 kb on + strandat 985.187 kb on + strandat 985.461 kb on + strand, within Pf6N2E2_840at 985.461 kb on + strand, within Pf6N2E2_840at 985.461 kb on + strand, within Pf6N2E2_840at 985.461 kb on + strand, within Pf6N2E2_840at 985.462 kb on - strand, within Pf6N2E2_840at 985.462 kb on - strand, within Pf6N2E2_840at 985.462 kb on - strand, within Pf6N2E2_840at 985.462 kb on - strand, within Pf6N2E2_840at 985.669 kb on + strand, within Pf6N2E2_840at 985.684 kb on + strand, within Pf6N2E2_840at 985.684 kb on + strand, within Pf6N2E2_840at 985.685 kb on - strand, within Pf6N2E2_840at 985.725 kb on + strand, within Pf6N2E2_840

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Per-strain Table

Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M
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981,408 - Pf6N2E2_838 0.54 -0.2
981,623 + Pf6N2E2_838 0.70 +0.3
981,738 - Pf6N2E2_838 0.79 +1.1
981,738 - Pf6N2E2_838 0.79 -0.2
981,738 - Pf6N2E2_838 0.79 -1.5
981,738 - Pf6N2E2_838 0.79 -0.0
981,738 - Pf6N2E2_838 0.79 +1.8
981,861 + Pf6N2E2_838 0.88 +0.3
981,861 + Pf6N2E2_838 0.88 -0.1
981,861 + Pf6N2E2_838 0.88 +0.2
981,871 + Pf6N2E2_838 0.89 -0.0
981,871 + Pf6N2E2_838 0.89 +1.2
981,871 + Pf6N2E2_838 0.89 -0.1
981,871 + Pf6N2E2_838 0.89 -0.3
981,917 + -0.1
981,917 + -2.8
981,918 - +1.0
982,013 + +0.3
982,013 + +1.3
982,013 + +1.8
982,014 - +0.6
982,048 + -0.7
982,048 + -0.1
982,048 + -0.6
982,091 + -1.3
982,139 - -0.4
982,158 - +0.3
982,163 + +1.0
982,223 + -0.7
982,224 - +0.1
982,224 - -1.3
982,224 - -2.5
982,252 + -0.6
982,274 + +1.7
982,275 - +0.7
982,313 + -0.1
982,313 + +0.7
982,313 + +0.2
982,331 + +1.1
982,332 - -0.8
982,332 - -2.8
982,332 - -1.3
982,343 + +0.9
982,343 + -0.0
982,343 + +0.4
982,343 + +1.0
982,344 - +0.9
982,344 - -1.9
982,375 + +0.1
982,375 + +0.2
982,480 + Pf6N2E2_839 0.13 -0.1
982,480 + Pf6N2E2_839 0.13 -0.3
982,589 + Pf6N2E2_839 0.17 -0.5
982,590 - Pf6N2E2_839 0.17 +0.4
982,595 + Pf6N2E2_839 0.17 -0.4
982,595 + Pf6N2E2_839 0.17 +0.2
982,705 + Pf6N2E2_839 0.22 -0.6
982,706 - Pf6N2E2_839 0.22 -0.7
982,718 + Pf6N2E2_839 0.22 +0.9
982,719 - Pf6N2E2_839 0.22 -0.4
982,730 + Pf6N2E2_839 0.23 +1.1
982,730 + Pf6N2E2_839 0.23 +0.8
982,730 + Pf6N2E2_839 0.23 +0.8
982,731 - Pf6N2E2_839 0.23 +1.4
982,731 - Pf6N2E2_839 0.23 +0.5
982,736 + Pf6N2E2_839 0.23 -0.2
982,826 + Pf6N2E2_839 0.26 +0.1
983,015 + Pf6N2E2_839 0.33 +2.3
983,016 - Pf6N2E2_839 0.33 +1.7
983,114 - Pf6N2E2_839 0.37 +1.3
983,114 - Pf6N2E2_839 0.37 +0.6
983,144 + Pf6N2E2_839 0.38 -2.1
983,144 + Pf6N2E2_839 0.38 +1.2
983,144 + Pf6N2E2_839 0.38 -0.7
983,144 + Pf6N2E2_839 0.38 -2.3
983,144 + Pf6N2E2_839 0.38 +0.8
983,144 + Pf6N2E2_839 0.38 +0.1
983,144 + Pf6N2E2_839 0.38 +0.3
983,145 - Pf6N2E2_839 0.38 +1.3
983,145 - Pf6N2E2_839 0.38 -0.3
983,145 - Pf6N2E2_839 0.38 +1.3
983,228 + Pf6N2E2_839 0.42 +0.2
983,336 - Pf6N2E2_839 0.46 +0.8
983,393 + Pf6N2E2_839 0.48 -1.3
983,393 + Pf6N2E2_839 0.48 +0.7
983,393 + Pf6N2E2_839 0.48 +1.4
983,393 + Pf6N2E2_839 0.48 -0.1
983,393 + Pf6N2E2_839 0.48 -1.0
983,393 + Pf6N2E2_839 0.48 -0.3
983,393 + Pf6N2E2_839 0.48 +1.0
983,393 + Pf6N2E2_839 0.48 +0.7
983,393 + Pf6N2E2_839 0.48 -2.5
983,394 - Pf6N2E2_839 0.48 -0.3
983,527 + Pf6N2E2_839 0.53 +1.4
983,570 - Pf6N2E2_839 0.55 +0.8
983,642 + Pf6N2E2_839 0.57 -1.4
983,642 + Pf6N2E2_839 0.57 +0.2
983,642 + Pf6N2E2_839 0.57 -0.7
983,642 + Pf6N2E2_839 0.57 +0.7
983,643 - +2.1
983,643 - Pf6N2E2_839 0.57 +1.3
983,643 - Pf6N2E2_839 0.57 +1.7
983,665 + Pf6N2E2_839 0.58 -0.1
983,666 - Pf6N2E2_839 0.58 +0.1
983,666 - Pf6N2E2_839 0.58 +1.8
984,085 - Pf6N2E2_839 0.74 +3.1
984,085 - Pf6N2E2_839 0.74 +0.1
984,085 - Pf6N2E2_839 0.74 +1.4
984,085 - Pf6N2E2_839 0.74 -0.9
984,478 + Pf6N2E2_839 0.89 +0.1
984,482 - Pf6N2E2_839 0.89 -1.0
984,615 + +0.5
984,671 + +0.2
984,671 + -1.8
984,671 + -0.1
984,671 + +2.9
984,736 + -1.9
984,736 + -0.0
984,934 + +1.2
984,934 + +0.3
984,935 - +0.3
984,935 - +0.4
984,935 - -0.3
984,938 + +0.3
984,981 + -1.2
985,006 + +3.9
985,007 - -1.0
985,007 - -0.4
985,007 - -0.3
985,007 - +0.1
985,018 + -0.9
985,018 + -1.3
985,115 - -1.3
985,116 + +1.1
985,117 - +1.3
985,117 - +0.3
985,158 + +0.9
985,171 + +0.3
985,187 + -0.1
985,461 + Pf6N2E2_840 0.13 +0.7
985,461 + Pf6N2E2_840 0.13 -1.0
985,461 + Pf6N2E2_840 0.13 +0.7
985,461 + Pf6N2E2_840 0.13 -0.3
985,462 - Pf6N2E2_840 0.13 -0.1
985,462 - Pf6N2E2_840 0.13 +0.4
985,462 - Pf6N2E2_840 0.13 +1.0
985,462 - Pf6N2E2_840 0.13 +0.6
985,669 + Pf6N2E2_840 0.34 -1.4
985,684 + Pf6N2E2_840 0.35 +0.3
985,684 + Pf6N2E2_840 0.35 +0.2
985,685 - Pf6N2E2_840 0.35 +0.6
985,725 + Pf6N2E2_840 0.40 +0.2

Or see this region's nucleotide sequence