Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5985 and Pf6N2E2_5986 are separated by 9 nucleotides Pf6N2E2_5986 and Pf6N2E2_5987 overlap by 4 nucleotides Pf6N2E2_5987 and Pf6N2E2_5988 are separated by 8 nucleotides Pf6N2E2_5988 and Pf6N2E2_5989 overlap by 24 nucleotides
Pf6N2E2_5985 - Extracellular solute-binding protein, family 3, at 6,804,244 to 6,805,068
_5985
Pf6N2E2_5986 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 6,805,078 to 6,805,437
_5986
Pf6N2E2_5987 - OmpA family protein, at 6,805,434 to 6,806,243
_5987
Pf6N2E2_5988 - hypothetical protein, at 6,806,252 to 6,806,467
_5988
Pf6N2E2_5989 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 6,806,444 to 6,807,886
_5989
Position (kb)
6805
6806
6807 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6804.469 kb on + strand, within Pf6N2E2_5985 at 6804.571 kb on + strand, within Pf6N2E2_5985 at 6804.571 kb on + strand, within Pf6N2E2_5985 at 6804.571 kb on + strand, within Pf6N2E2_5985 at 6804.571 kb on + strand, within Pf6N2E2_5985 at 6804.572 kb on - strand, within Pf6N2E2_5985 at 6804.601 kb on + strand, within Pf6N2E2_5985 at 6804.693 kb on + strand, within Pf6N2E2_5985 at 6804.694 kb on - strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.796 kb on + strand, within Pf6N2E2_5985 at 6804.797 kb on - strand, within Pf6N2E2_5985 at 6804.797 kb on - strand, within Pf6N2E2_5985 at 6804.797 kb on - strand, within Pf6N2E2_5985 at 6804.797 kb on - strand, within Pf6N2E2_5985 at 6804.797 kb on - strand, within Pf6N2E2_5985 at 6804.915 kb on + strand, within Pf6N2E2_5985 at 6805.076 kb on + strand at 6805.076 kb on + strand at 6805.077 kb on - strand at 6805.083 kb on + strand at 6805.084 kb on - strand at 6805.285 kb on + strand, within Pf6N2E2_5986 at 6805.285 kb on + strand, within Pf6N2E2_5986 at 6805.285 kb on + strand, within Pf6N2E2_5986 at 6805.450 kb on + strand at 6805.450 kb on + strand at 6805.450 kb on + strand at 6805.451 kb on - strand at 6805.466 kb on - strand at 6805.488 kb on + strand at 6805.488 kb on + strand at 6805.488 kb on + strand at 6805.488 kb on + strand at 6805.488 kb on + strand at 6805.489 kb on - strand at 6805.641 kb on + strand, within Pf6N2E2_5987 at 6805.671 kb on + strand, within Pf6N2E2_5987 at 6805.678 kb on - strand, within Pf6N2E2_5987 at 6805.692 kb on + strand, within Pf6N2E2_5987 at 6805.693 kb on - strand, within Pf6N2E2_5987 at 6805.972 kb on + strand, within Pf6N2E2_5987 at 6805.972 kb on + strand, within Pf6N2E2_5987 at 6805.972 kb on + strand, within Pf6N2E2_5987 at 6806.010 kb on + strand, within Pf6N2E2_5987 at 6806.010 kb on + strand, within Pf6N2E2_5987 at 6806.010 kb on + strand, within Pf6N2E2_5987 at 6806.011 kb on - strand, within Pf6N2E2_5987 at 6806.011 kb on - strand, within Pf6N2E2_5987 at 6806.130 kb on + strand, within Pf6N2E2_5987 at 6806.275 kb on - strand, within Pf6N2E2_5988 at 6806.320 kb on + strand, within Pf6N2E2_5988 at 6806.320 kb on + strand, within Pf6N2E2_5988 at 6806.326 kb on + strand, within Pf6N2E2_5988 at 6806.327 kb on - strand, within Pf6N2E2_5988 at 6806.327 kb on - strand, within Pf6N2E2_5988 at 6806.327 kb on - strand, within Pf6N2E2_5988 at 6806.346 kb on - strand, within Pf6N2E2_5988 at 6806.415 kb on - strand, within Pf6N2E2_5988 at 6806.462 kb on + strand at 6806.463 kb on - strand at 6806.470 kb on + strand at 6806.470 kb on + strand at 6806.470 kb on + strand at 6806.470 kb on + strand at 6806.471 kb on - strand at 6806.471 kb on - strand at 6806.471 kb on - strand at 6806.471 kb on - strand at 6806.471 kb on - strand at 6806.471 kb on - strand at 6806.510 kb on + strand at 6806.510 kb on + strand at 6806.592 kb on - strand, within Pf6N2E2_5989 at 6806.642 kb on + strand, within Pf6N2E2_5989 at 6806.643 kb on - strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.645 kb on + strand, within Pf6N2E2_5989 at 6806.646 kb on - strand, within Pf6N2E2_5989 at 6806.646 kb on - strand, within Pf6N2E2_5989 at 6806.646 kb on - strand, within Pf6N2E2_5989 at 6806.646 kb on - strand, within Pf6N2E2_5989 at 6806.646 kb on - strand, within Pf6N2E2_5989 at 6806.743 kb on - strand, within Pf6N2E2_5989 at 6806.743 kb on - strand, within Pf6N2E2_5989 at 6806.804 kb on + strand, within Pf6N2E2_5989 at 6806.805 kb on - strand, within Pf6N2E2_5989 at 6806.805 kb on - strand, within Pf6N2E2_5989 at 6806.805 kb on - strand, within Pf6N2E2_5989 at 6806.805 kb on - strand, within Pf6N2E2_5989 at 6806.876 kb on + strand, within Pf6N2E2_5989 at 6806.876 kb on + strand, within Pf6N2E2_5989 at 6806.876 kb on + strand, within Pf6N2E2_5989 at 6806.877 kb on - strand, within Pf6N2E2_5989 at 6806.877 kb on - strand, within Pf6N2E2_5989 at 6806.877 kb on - strand, within Pf6N2E2_5989 at 6806.877 kb on - strand, within Pf6N2E2_5989 at 6806.877 kb on - strand, within Pf6N2E2_5989 at 6806.877 kb on - strand, within Pf6N2E2_5989 at 6807.056 kb on + strand, within Pf6N2E2_5989 at 6807.056 kb on + strand, within Pf6N2E2_5989 at 6807.057 kb on - strand, within Pf6N2E2_5989 at 6807.095 kb on - strand, within Pf6N2E2_5989 at 6807.130 kb on + strand, within Pf6N2E2_5989
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 6,804,469 + Pf6N2E2_5985 0.27 +0.7 6,804,571 + Pf6N2E2_5985 0.40 -0.8 6,804,571 + Pf6N2E2_5985 0.40 -0.2 6,804,571 + Pf6N2E2_5985 0.40 -0.3 6,804,571 + Pf6N2E2_5985 0.40 +1.5 6,804,572 - Pf6N2E2_5985 0.40 +0.4 6,804,601 + Pf6N2E2_5985 0.43 +0.3 6,804,693 + Pf6N2E2_5985 0.54 +0.7 6,804,694 - Pf6N2E2_5985 0.55 +0.8 6,804,796 + Pf6N2E2_5985 0.67 +1.2 6,804,796 + Pf6N2E2_5985 0.67 +0.4 6,804,796 + Pf6N2E2_5985 0.67 -2.4 6,804,796 + Pf6N2E2_5985 0.67 +1.0 6,804,796 + Pf6N2E2_5985 0.67 +1.8 6,804,796 + Pf6N2E2_5985 0.67 -0.9 6,804,796 + Pf6N2E2_5985 0.67 +0.4 6,804,796 + Pf6N2E2_5985 0.67 -0.7 6,804,796 + Pf6N2E2_5985 0.67 -0.3 6,804,797 - Pf6N2E2_5985 0.67 +0.3 6,804,797 - Pf6N2E2_5985 0.67 -1.6 6,804,797 - Pf6N2E2_5985 0.67 +0.3 6,804,797 - Pf6N2E2_5985 0.67 -0.1 6,804,797 - Pf6N2E2_5985 0.67 -0.0 6,804,915 + Pf6N2E2_5985 0.81 +0.2 6,805,076 + +0.6 6,805,076 + -0.5 6,805,077 - +0.6 6,805,083 + -2.4 6,805,084 - -0.7 6,805,285 + Pf6N2E2_5986 0.57 -1.9 6,805,285 + Pf6N2E2_5986 0.57 -1.7 6,805,285 + Pf6N2E2_5986 0.57 -2.1 6,805,450 + -0.2 6,805,450 + -1.5 6,805,450 + -1.2 6,805,451 - +0.3 6,805,466 - -1.5 6,805,488 + -0.9 6,805,488 + -2.3 6,805,488 + -0.7 6,805,488 + +0.1 6,805,488 + -1.0 6,805,489 - -1.4 6,805,641 + Pf6N2E2_5987 0.26 -1.0 6,805,671 + Pf6N2E2_5987 0.29 -1.7 6,805,678 - Pf6N2E2_5987 0.30 -2.2 6,805,692 + Pf6N2E2_5987 0.32 +0.2 6,805,693 - Pf6N2E2_5987 0.32 -0.2 6,805,972 + Pf6N2E2_5987 0.66 -1.2 6,805,972 + Pf6N2E2_5987 0.66 -0.9 6,805,972 + Pf6N2E2_5987 0.66 -0.9 6,806,010 + Pf6N2E2_5987 0.71 -0.5 6,806,010 + Pf6N2E2_5987 0.71 -1.5 6,806,010 + Pf6N2E2_5987 0.71 -1.4 6,806,011 - Pf6N2E2_5987 0.71 +0.4 6,806,011 - Pf6N2E2_5987 0.71 -1.8 6,806,130 + Pf6N2E2_5987 0.86 -0.2 6,806,275 - Pf6N2E2_5988 0.11 +1.3 6,806,320 + Pf6N2E2_5988 0.31 -0.9 6,806,320 + Pf6N2E2_5988 0.31 +0.6 6,806,326 + Pf6N2E2_5988 0.34 -0.5 6,806,327 - Pf6N2E2_5988 0.35 -0.2 6,806,327 - Pf6N2E2_5988 0.35 -1.2 6,806,327 - Pf6N2E2_5988 0.35 -0.2 6,806,346 - Pf6N2E2_5988 0.44 +0.2 6,806,415 - Pf6N2E2_5988 0.75 +0.5 6,806,462 + -1.0 6,806,463 - -0.6 6,806,470 + +0.6 6,806,470 + -0.7 6,806,470 + +0.1 6,806,470 + -0.3 6,806,471 - -0.3 6,806,471 - -2.1 6,806,471 - -0.8 6,806,471 - -0.3 6,806,471 - +0.5 6,806,471 - -1.0 6,806,510 + +0.3 6,806,510 + -0.7 6,806,592 - Pf6N2E2_5989 0.10 -0.6 6,806,642 + Pf6N2E2_5989 0.14 -0.1 6,806,643 - Pf6N2E2_5989 0.14 -1.5 6,806,645 + Pf6N2E2_5989 0.14 -1.3 6,806,645 + Pf6N2E2_5989 0.14 -0.4 6,806,645 + Pf6N2E2_5989 0.14 -1.2 6,806,645 + Pf6N2E2_5989 0.14 -0.2 6,806,645 + Pf6N2E2_5989 0.14 -1.3 6,806,645 + Pf6N2E2_5989 0.14 -0.5 6,806,645 + Pf6N2E2_5989 0.14 -0.9 6,806,646 - Pf6N2E2_5989 0.14 -0.6 6,806,646 - Pf6N2E2_5989 0.14 -0.2 6,806,646 - Pf6N2E2_5989 0.14 +0.8 6,806,646 - Pf6N2E2_5989 0.14 -0.3 6,806,646 - Pf6N2E2_5989 0.14 +0.3 6,806,743 - Pf6N2E2_5989 0.21 +1.6 6,806,743 - Pf6N2E2_5989 0.21 +1.3 6,806,804 + Pf6N2E2_5989 0.25 +0.1 6,806,805 - Pf6N2E2_5989 0.25 +0.4 6,806,805 - Pf6N2E2_5989 0.25 -1.3 6,806,805 - Pf6N2E2_5989 0.25 +0.5 6,806,805 - Pf6N2E2_5989 0.25 -0.4 6,806,876 + Pf6N2E2_5989 0.30 -0.2 6,806,876 + Pf6N2E2_5989 0.30 -0.2 6,806,876 + Pf6N2E2_5989 0.30 -0.4 6,806,877 - Pf6N2E2_5989 0.30 -0.4 6,806,877 - Pf6N2E2_5989 0.30 -3.4 6,806,877 - Pf6N2E2_5989 0.30 -1.9 6,806,877 - Pf6N2E2_5989 0.30 -2.9 6,806,877 - Pf6N2E2_5989 0.30 -0.5 6,806,877 - Pf6N2E2_5989 0.30 +1.6 6,807,056 + Pf6N2E2_5989 0.42 +1.4 6,807,056 + Pf6N2E2_5989 0.42 -1.3 6,807,057 - Pf6N2E2_5989 0.42 -2.6 6,807,095 - Pf6N2E2_5989 0.45 -0.7 6,807,130 + Pf6N2E2_5989 0.48 +0.2
Or see this region's nucleotide sequence