Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5751 and Pf6N2E2_5752 overlap by 4 nucleotides Pf6N2E2_5752 and Pf6N2E2_5753 are separated by 64 nucleotides Pf6N2E2_5753 and Pf6N2E2_5754 are separated by 88 nucleotides Pf6N2E2_5754 and Pf6N2E2_5755 are separated by 144 nucleotides Pf6N2E2_5755 and Pf6N2E2_5756 are separated by 276 nucleotides
Pf6N2E2_5751 - Permeases of the major facilitator superfamily, at 6,565,022 to 6,566,230
_5751
Pf6N2E2_5752 - hypothetical protein, at 6,566,227 to 6,566,346
_5752
Pf6N2E2_5753 - Glutathione peroxidase family protein, at 6,566,411 to 6,566,962
_5753
Pf6N2E2_5754 - Long-chain fatty acid transport protein, at 6,567,051 to 6,568,325
_5754
Pf6N2E2_5755 - FIG00956435: hypothetical protein, at 6,568,470 to 6,568,952
_5755
Pf6N2E2_5756 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 6,569,229 to 6,569,453
_5756
Position (kb)
6567
6568
6569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6566.099 kb on + strand, within Pf6N2E2_5751 at 6566.099 kb on + strand, within Pf6N2E2_5751 at 6566.117 kb on + strand at 6566.117 kb on + strand at 6566.118 kb on - strand at 6566.181 kb on + strand at 6566.181 kb on + strand at 6566.182 kb on - strand at 6566.182 kb on - strand at 6566.373 kb on + strand at 6566.397 kb on + strand at 6566.515 kb on - strand, within Pf6N2E2_5753 at 6566.515 kb on - strand, within Pf6N2E2_5753 at 6566.515 kb on - strand, within Pf6N2E2_5753 at 6566.730 kb on - strand, within Pf6N2E2_5753 at 6566.730 kb on - strand, within Pf6N2E2_5753 at 6566.730 kb on - strand, within Pf6N2E2_5753 at 6566.730 kb on - strand, within Pf6N2E2_5753 at 6566.738 kb on + strand, within Pf6N2E2_5753 at 6566.793 kb on + strand, within Pf6N2E2_5753 at 6566.852 kb on + strand, within Pf6N2E2_5753 at 6566.852 kb on + strand, within Pf6N2E2_5753 at 6566.852 kb on + strand, within Pf6N2E2_5753 at 6566.852 kb on + strand, within Pf6N2E2_5753 at 6566.853 kb on - strand, within Pf6N2E2_5753 at 6566.853 kb on - strand, within Pf6N2E2_5753 at 6566.934 kb on + strand at 6566.934 kb on + strand at 6566.934 kb on + strand at 6566.934 kb on + strand at 6566.935 kb on - strand at 6566.935 kb on - strand at 6566.935 kb on - strand at 6566.935 kb on - strand at 6567.062 kb on - strand at 6567.112 kb on + strand at 6567.112 kb on + strand at 6567.113 kb on - strand at 6567.113 kb on - strand at 6567.113 kb on - strand at 6567.113 kb on - strand at 6567.172 kb on + strand at 6567.172 kb on + strand at 6567.172 kb on + strand at 6567.173 kb on - strand at 6567.335 kb on - strand, within Pf6N2E2_5754 at 6567.352 kb on + strand, within Pf6N2E2_5754 at 6567.411 kb on + strand, within Pf6N2E2_5754 at 6567.493 kb on + strand, within Pf6N2E2_5754 at 6567.493 kb on + strand, within Pf6N2E2_5754 at 6567.493 kb on + strand, within Pf6N2E2_5754 at 6567.494 kb on - strand, within Pf6N2E2_5754 at 6567.494 kb on - strand, within Pf6N2E2_5754 at 6567.574 kb on + strand, within Pf6N2E2_5754 at 6567.646 kb on + strand, within Pf6N2E2_5754 at 6567.646 kb on + strand, within Pf6N2E2_5754 at 6567.646 kb on + strand, within Pf6N2E2_5754 at 6567.646 kb on + strand, within Pf6N2E2_5754 at 6567.647 kb on - strand, within Pf6N2E2_5754 at 6567.647 kb on - strand, within Pf6N2E2_5754 at 6567.664 kb on + strand, within Pf6N2E2_5754 at 6567.716 kb on + strand, within Pf6N2E2_5754 at 6567.718 kb on + strand, within Pf6N2E2_5754 at 6567.719 kb on - strand, within Pf6N2E2_5754 at 6567.719 kb on - strand, within Pf6N2E2_5754 at 6567.725 kb on - strand, within Pf6N2E2_5754 at 6567.750 kb on + strand, within Pf6N2E2_5754 at 6567.750 kb on + strand, within Pf6N2E2_5754 at 6567.751 kb on - strand, within Pf6N2E2_5754 at 6567.806 kb on + strand, within Pf6N2E2_5754 at 6567.806 kb on + strand, within Pf6N2E2_5754 at 6567.807 kb on - strand, within Pf6N2E2_5754 at 6567.807 kb on - strand, within Pf6N2E2_5754 at 6567.807 kb on - strand, within Pf6N2E2_5754 at 6567.838 kb on - strand, within Pf6N2E2_5754 at 6567.856 kb on + strand, within Pf6N2E2_5754 at 6567.856 kb on + strand, within Pf6N2E2_5754 at 6567.856 kb on + strand, within Pf6N2E2_5754 at 6567.856 kb on + strand, within Pf6N2E2_5754 at 6567.856 kb on + strand, within Pf6N2E2_5754 at 6567.857 kb on - strand, within Pf6N2E2_5754 at 6567.857 kb on - strand, within Pf6N2E2_5754 at 6567.857 kb on - strand, within Pf6N2E2_5754 at 6567.857 kb on - strand, within Pf6N2E2_5754 at 6567.962 kb on - strand, within Pf6N2E2_5754 at 6567.996 kb on - strand, within Pf6N2E2_5754 at 6567.996 kb on - strand, within Pf6N2E2_5754 at 6568.006 kb on + strand, within Pf6N2E2_5754 at 6568.006 kb on + strand, within Pf6N2E2_5754 at 6568.006 kb on + strand, within Pf6N2E2_5754 at 6568.006 kb on + strand, within Pf6N2E2_5754 at 6568.006 kb on + strand, within Pf6N2E2_5754 at 6568.007 kb on - strand, within Pf6N2E2_5754 at 6568.007 kb on - strand, within Pf6N2E2_5754 at 6568.007 kb on - strand, within Pf6N2E2_5754 at 6568.007 kb on - strand, within Pf6N2E2_5754 at 6568.007 kb on - strand, within Pf6N2E2_5754 at 6568.007 kb on - strand, within Pf6N2E2_5754 at 6568.009 kb on + strand, within Pf6N2E2_5754 at 6568.009 kb on + strand, within Pf6N2E2_5754 at 6568.329 kb on + strand at 6568.329 kb on + strand at 6568.330 kb on - strand at 6568.361 kb on - strand at 6568.405 kb on - strand at 6568.716 kb on + strand, within Pf6N2E2_5755 at 6568.717 kb on - strand, within Pf6N2E2_5755 at 6568.750 kb on + strand, within Pf6N2E2_5755 at 6568.750 kb on + strand, within Pf6N2E2_5755 at 6568.751 kb on - strand, within Pf6N2E2_5755 at 6568.897 kb on + strand, within Pf6N2E2_5755 at 6568.921 kb on + strand at 6568.921 kb on + strand at 6568.921 kb on + strand at 6568.921 kb on + strand at 6568.921 kb on + strand at 6568.952 kb on + strand at 6568.952 kb on + strand at 6568.952 kb on + strand at 6569.068 kb on + strand at 6569.117 kb on + strand at 6569.117 kb on + strand at 6569.117 kb on + strand at 6569.124 kb on + strand at 6569.124 kb on + strand at 6569.125 kb on - strand at 6569.137 kb on - strand at 6569.253 kb on - strand, within Pf6N2E2_5756 at 6569.253 kb on - strand, within Pf6N2E2_5756 at 6569.291 kb on - strand, within Pf6N2E2_5756 at 6569.291 kb on - strand, within Pf6N2E2_5756
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 6,566,099 + Pf6N2E2_5751 0.89 +0.1 6,566,099 + Pf6N2E2_5751 0.89 +0.0 6,566,117 + -1.1 6,566,117 + +0.5 6,566,118 - +0.3 6,566,181 + +0.6 6,566,181 + +0.7 6,566,182 - -1.0 6,566,182 - +0.1 6,566,373 + -0.8 6,566,397 + -0.0 6,566,515 - Pf6N2E2_5753 0.19 +0.1 6,566,515 - Pf6N2E2_5753 0.19 -3.1 6,566,515 - Pf6N2E2_5753 0.19 -0.0 6,566,730 - Pf6N2E2_5753 0.58 -0.6 6,566,730 - Pf6N2E2_5753 0.58 +0.1 6,566,730 - Pf6N2E2_5753 0.58 +1.3 6,566,730 - Pf6N2E2_5753 0.58 +0.1 6,566,738 + Pf6N2E2_5753 0.59 +0.1 6,566,793 + Pf6N2E2_5753 0.69 +1.0 6,566,852 + Pf6N2E2_5753 0.80 -1.2 6,566,852 + Pf6N2E2_5753 0.80 +1.2 6,566,852 + Pf6N2E2_5753 0.80 +1.0 6,566,852 + Pf6N2E2_5753 0.80 -0.6 6,566,853 - Pf6N2E2_5753 0.80 -0.8 6,566,853 - Pf6N2E2_5753 0.80 -1.1 6,566,934 + +1.0 6,566,934 + -1.5 6,566,934 + +0.8 6,566,934 + +0.1 6,566,935 - -0.0 6,566,935 - -1.3 6,566,935 - +2.6 6,566,935 - +0.9 6,567,062 - -3.4 6,567,112 + +0.3 6,567,112 + -2.5 6,567,113 - -1.7 6,567,113 - -0.9 6,567,113 - +0.6 6,567,113 - -2.8 6,567,172 + -2.2 6,567,172 + -1.2 6,567,172 + -1.2 6,567,173 - -1.6 6,567,335 - Pf6N2E2_5754 0.22 -2.5 6,567,352 + Pf6N2E2_5754 0.24 -0.7 6,567,411 + Pf6N2E2_5754 0.28 -0.8 6,567,493 + Pf6N2E2_5754 0.35 -0.8 6,567,493 + Pf6N2E2_5754 0.35 -0.3 6,567,493 + Pf6N2E2_5754 0.35 -0.4 6,567,494 - Pf6N2E2_5754 0.35 -0.7 6,567,494 - Pf6N2E2_5754 0.35 -1.7 6,567,574 + Pf6N2E2_5754 0.41 -0.7 6,567,646 + Pf6N2E2_5754 0.47 -1.7 6,567,646 + Pf6N2E2_5754 0.47 -1.6 6,567,646 + Pf6N2E2_5754 0.47 -2.2 6,567,646 + Pf6N2E2_5754 0.47 -1.3 6,567,647 - Pf6N2E2_5754 0.47 -3.2 6,567,647 - Pf6N2E2_5754 0.47 -0.5 6,567,664 + Pf6N2E2_5754 0.48 -1.9 6,567,716 + Pf6N2E2_5754 0.52 -0.9 6,567,718 + Pf6N2E2_5754 0.52 -1.6 6,567,719 - Pf6N2E2_5754 0.52 -2.9 6,567,719 - Pf6N2E2_5754 0.52 -1.8 6,567,725 - Pf6N2E2_5754 0.53 -0.1 6,567,750 + Pf6N2E2_5754 0.55 -2.0 6,567,750 + Pf6N2E2_5754 0.55 -1.0 6,567,751 - Pf6N2E2_5754 0.55 -1.1 6,567,806 + Pf6N2E2_5754 0.59 +0.1 6,567,806 + Pf6N2E2_5754 0.59 -1.0 6,567,807 - Pf6N2E2_5754 0.59 -1.6 6,567,807 - Pf6N2E2_5754 0.59 -2.3 6,567,807 - Pf6N2E2_5754 0.59 -1.1 6,567,838 - Pf6N2E2_5754 0.62 -1.2 6,567,856 + Pf6N2E2_5754 0.63 +0.1 6,567,856 + Pf6N2E2_5754 0.63 -1.5 6,567,856 + Pf6N2E2_5754 0.63 -2.3 6,567,856 + Pf6N2E2_5754 0.63 +0.5 6,567,856 + Pf6N2E2_5754 0.63 -1.4 6,567,857 - Pf6N2E2_5754 0.63 -2.3 6,567,857 - Pf6N2E2_5754 0.63 -1.5 6,567,857 - Pf6N2E2_5754 0.63 -1.3 6,567,857 - Pf6N2E2_5754 0.63 -1.6 6,567,962 - Pf6N2E2_5754 0.71 -2.5 6,567,996 - Pf6N2E2_5754 0.74 -1.9 6,567,996 - Pf6N2E2_5754 0.74 +0.1 6,568,006 + Pf6N2E2_5754 0.75 -3.2 6,568,006 + Pf6N2E2_5754 0.75 -0.6 6,568,006 + Pf6N2E2_5754 0.75 -0.9 6,568,006 + Pf6N2E2_5754 0.75 -1.3 6,568,006 + Pf6N2E2_5754 0.75 +0.1 6,568,007 - Pf6N2E2_5754 0.75 -2.9 6,568,007 - Pf6N2E2_5754 0.75 -1.3 6,568,007 - Pf6N2E2_5754 0.75 -2.0 6,568,007 - Pf6N2E2_5754 0.75 +0.1 6,568,007 - Pf6N2E2_5754 0.75 -0.5 6,568,007 - Pf6N2E2_5754 0.75 -1.1 6,568,009 + Pf6N2E2_5754 0.75 -0.6 6,568,009 + Pf6N2E2_5754 0.75 -2.5 6,568,329 + +1.3 6,568,329 + -0.2 6,568,330 - -0.9 6,568,361 - -0.5 6,568,405 - +0.4 6,568,716 + Pf6N2E2_5755 0.51 +0.1 6,568,717 - Pf6N2E2_5755 0.51 -0.5 6,568,750 + Pf6N2E2_5755 0.58 -0.2 6,568,750 + Pf6N2E2_5755 0.58 +1.9 6,568,751 - Pf6N2E2_5755 0.58 +0.9 6,568,897 + Pf6N2E2_5755 0.88 -0.1 6,568,921 + +1.0 6,568,921 + +0.2 6,568,921 + -0.5 6,568,921 + +1.0 6,568,921 + -0.9 6,568,952 + +0.3 6,568,952 + +0.5 6,568,952 + -0.6 6,569,068 + -0.5 6,569,117 + +0.9 6,569,117 + -0.1 6,569,117 + -0.2 6,569,124 + -0.4 6,569,124 + +0.3 6,569,125 - +0.9 6,569,137 - +0.7 6,569,253 - Pf6N2E2_5756 0.11 +0.0 6,569,253 - Pf6N2E2_5756 0.11 +0.6 6,569,291 - Pf6N2E2_5756 0.28 -0.0 6,569,291 - Pf6N2E2_5756 0.28 +0.1
Or see this region's nucleotide sequence