Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5186 and Pf6N2E2_5187 are separated by 82 nucleotides Pf6N2E2_5187 and Pf6N2E2_5188 overlap by 8 nucleotides Pf6N2E2_5188 and Pf6N2E2_5189 are separated by 51 nucleotides Pf6N2E2_5189 and Pf6N2E2_5190 are separated by 132 nucleotides
Pf6N2E2_5186 - FIG00954491: hypothetical protein, at 5,982,488 to 5,983,867
_5186
Pf6N2E2_5187 - Periplasmic protein TonB, links inner and outer membranes, at 5,983,950 to 5,984,729
_5187
Pf6N2E2_5188 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 5,984,722 to 5,985,174
_5188
Pf6N2E2_5189 - Phosphotransferase system, HPr-related proteins, at 5,985,226 to 5,985,630
_5189
Pf6N2E2_5190 - Carbonic anhydrase (EC 4.2.1.1), at 5,985,763 to 5,986,407
_5190
Position (kb)
5984
5985
5986 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5983.793 kb on + strand at 5983.794 kb on - strand at 5983.794 kb on - strand at 5983.794 kb on - strand at 5983.808 kb on + strand at 5983.869 kb on + strand at 5983.870 kb on - strand at 5983.870 kb on - strand at 5983.870 kb on - strand at 5983.908 kb on + strand at 5984.735 kb on + strand at 5984.736 kb on - strand at 5984.788 kb on + strand, within Pf6N2E2_5188 at 5984.788 kb on + strand, within Pf6N2E2_5188 at 5984.797 kb on + strand, within Pf6N2E2_5188 at 5984.798 kb on - strand, within Pf6N2E2_5188 at 5984.846 kb on - strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.005 kb on - strand, within Pf6N2E2_5188 at 5985.005 kb on - strand, within Pf6N2E2_5188 at 5985.070 kb on + strand, within Pf6N2E2_5188 at 5985.070 kb on + strand, within Pf6N2E2_5188 at 5985.112 kb on + strand, within Pf6N2E2_5188 at 5985.295 kb on - strand, within Pf6N2E2_5189 at 5985.643 kb on + strand at 5985.644 kb on - strand at 5985.655 kb on + strand at 5985.655 kb on + strand at 5985.656 kb on - strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.773 kb on + strand at 5985.773 kb on + strand at 5985.773 kb on + strand at 5985.774 kb on - strand at 5985.790 kb on - strand at 5985.871 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5986.141 kb on + strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 5,983,793 + +0.8 5,983,794 - +0.4 5,983,794 - +0.2 5,983,794 - -1.2 5,983,808 + +0.1 5,983,869 + +1.7 5,983,870 - -0.9 5,983,870 - -1.6 5,983,870 - +0.9 5,983,908 + -2.2 5,984,735 + +0.8 5,984,736 - -0.3 5,984,788 + Pf6N2E2_5188 0.15 +0.8 5,984,788 + Pf6N2E2_5188 0.15 -2.1 5,984,797 + Pf6N2E2_5188 0.17 -2.8 5,984,798 - Pf6N2E2_5188 0.17 +0.4 5,984,846 - Pf6N2E2_5188 0.27 -1.3 5,985,004 + Pf6N2E2_5188 0.62 -1.1 5,985,004 + Pf6N2E2_5188 0.62 -0.8 5,985,004 + Pf6N2E2_5188 0.62 -2.2 5,985,005 - Pf6N2E2_5188 0.62 -2.3 5,985,005 - Pf6N2E2_5188 0.62 -0.0 5,985,070 + Pf6N2E2_5188 0.77 +1.0 5,985,070 + Pf6N2E2_5188 0.77 -0.4 5,985,112 + Pf6N2E2_5188 0.86 -0.0 5,985,295 - Pf6N2E2_5189 0.17 -0.6 5,985,643 + -0.1 5,985,644 - +0.5 5,985,655 + +1.5 5,985,655 + +0.3 5,985,656 - -0.8 5,985,742 + -0.4 5,985,742 + +0.2 5,985,742 + +0.7 5,985,742 + -0.4 5,985,773 + -0.4 5,985,773 + +0.1 5,985,773 + +0.4 5,985,774 - -1.8 5,985,790 - -0.5 5,985,871 + Pf6N2E2_5190 0.17 +0.0 5,985,947 + Pf6N2E2_5190 0.29 -0.1 5,985,947 + Pf6N2E2_5190 0.29 -1.0 5,985,947 + Pf6N2E2_5190 0.29 -1.8 5,985,947 + Pf6N2E2_5190 0.29 -0.3 5,985,948 - Pf6N2E2_5190 0.29 +0.5 5,985,948 - Pf6N2E2_5190 0.29 +0.6 5,985,948 - Pf6N2E2_5190 0.29 -0.7 5,985,968 + Pf6N2E2_5190 0.32 -2.5 5,985,968 + Pf6N2E2_5190 0.32 -1.3 5,985,968 + Pf6N2E2_5190 0.32 -0.6 5,985,968 + Pf6N2E2_5190 0.32 -0.7 5,985,968 + Pf6N2E2_5190 0.32 -0.2 5,985,968 + Pf6N2E2_5190 0.32 -0.8 5,985,968 + Pf6N2E2_5190 0.32 -0.4 5,985,969 - Pf6N2E2_5190 0.32 -2.4 5,985,969 - Pf6N2E2_5190 0.32 -0.4 5,985,969 - Pf6N2E2_5190 0.32 +0.7 5,985,969 - Pf6N2E2_5190 0.32 +0.1 5,985,969 - Pf6N2E2_5190 0.32 +0.0 5,985,969 - Pf6N2E2_5190 0.32 +0.1 5,985,969 - Pf6N2E2_5190 0.32 -1.8 5,985,969 - Pf6N2E2_5190 0.32 +1.6 5,986,141 + Pf6N2E2_5190 0.59 +0.8 5,986,142 - Pf6N2E2_5190 0.59 -0.3 5,986,142 - Pf6N2E2_5190 0.59 +0.5 5,986,142 - Pf6N2E2_5190 0.59 +1.0 5,986,142 - Pf6N2E2_5190 0.59 +0.8 5,986,144 + Pf6N2E2_5190 0.59 +0.2 5,986,144 + Pf6N2E2_5190 0.59 +0.4 5,986,144 + Pf6N2E2_5190 0.59 +0.0 5,986,144 + Pf6N2E2_5190 0.59 -0.1 5,986,145 - Pf6N2E2_5190 0.59 -0.7 5,986,145 - Pf6N2E2_5190 0.59 -0.0 5,986,145 - Pf6N2E2_5190 0.59 +0.3
Or see this region's nucleotide sequence