Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_503 and Pf6N2E2_504 overlap by 23 nucleotides Pf6N2E2_504 and Pf6N2E2_505 are separated by 24 nucleotides Pf6N2E2_505 and Pf6N2E2_506 overlap by 35 nucleotides Pf6N2E2_506 and Pf6N2E2_507 overlap by 4 nucleotides
Pf6N2E2_503 - sensor histidine kinase, at 572,701 to 574,086
_503
Pf6N2E2_504 - DNA-binding response regulator, at 574,064 to 574,747
_504
Pf6N2E2_505 - hypothetical protein, at 574,772 to 575,053
_505
Pf6N2E2_506 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 575,019 to 575,342
_506
Pf6N2E2_507 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 575,339 to 576,748
_507
Position (kb) 574
575 Strain fitness (log2 ratio) -1
0
1
2 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.133 kb on + strand, within Pf6N2E2_503 at 573.652 kb on + strand, within Pf6N2E2_503 at 573.967 kb on + strand at 573.968 kb on - strand at 573.968 kb on - strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.975 kb on - strand at 573.975 kb on - strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 574.025 kb on + strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.197 kb on + strand, within Pf6N2E2_504 at 574.276 kb on - strand, within Pf6N2E2_504 at 574.361 kb on + strand, within Pf6N2E2_504 at 574.612 kb on - strand, within Pf6N2E2_504 at 574.776 kb on + strand at 574.776 kb on + strand at 574.802 kb on - strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.890 kb on + strand, within Pf6N2E2_505 at 574.932 kb on + strand, within Pf6N2E2_505 at 574.933 kb on - strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.038 kb on + strand at 575.038 kb on + strand at 575.039 kb on - strand at 575.202 kb on + strand, within Pf6N2E2_506 at 575.202 kb on + strand, within Pf6N2E2_506 at 575.218 kb on + strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.498 kb on + strand, within Pf6N2E2_507
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 573,110 + Pf6N2E2_503 0.30 -0.9 573,110 + Pf6N2E2_503 0.30 +1.6 573,133 + Pf6N2E2_503 0.31 +1.4 573,652 + Pf6N2E2_503 0.69 +1.1 573,967 + -1.0 573,968 - -0.9 573,968 - +0.4 573,974 + +1.4 573,974 + -0.4 573,974 + -0.2 573,975 - -1.0 573,975 - -0.5 573,977 + +0.1 573,977 + -0.2 573,977 + -1.4 573,977 + +1.0 573,977 + +0.8 573,978 - +0.0 573,978 - +1.4 573,978 - -0.4 573,978 - -0.6 573,978 - -0.2 574,025 + -1.2 574,026 - +0.3 574,026 - -0.3 574,026 - -0.5 574,197 + Pf6N2E2_504 0.19 +0.1 574,276 - Pf6N2E2_504 0.31 -1.0 574,361 + Pf6N2E2_504 0.43 -1.1 574,612 - Pf6N2E2_504 0.80 -1.1 574,776 + +0.2 574,776 + -0.3 574,802 - Pf6N2E2_505 0.11 +0.4 574,878 + Pf6N2E2_505 0.38 -0.0 574,878 + Pf6N2E2_505 0.38 -0.6 574,878 + Pf6N2E2_505 0.38 +0.9 574,890 + Pf6N2E2_505 0.42 -0.7 574,932 + Pf6N2E2_505 0.57 -0.3 574,933 - Pf6N2E2_505 0.57 -0.7 575,015 + Pf6N2E2_505 0.86 +1.6 575,015 + Pf6N2E2_505 0.86 +0.7 575,015 + Pf6N2E2_505 0.86 +0.1 575,015 + Pf6N2E2_505 0.86 +1.0 575,016 - Pf6N2E2_505 0.87 +0.6 575,016 - Pf6N2E2_505 0.87 -0.2 575,016 - Pf6N2E2_505 0.87 +0.7 575,016 - Pf6N2E2_505 0.87 +0.6 575,038 + +1.0 575,038 + -0.8 575,039 - -0.4 575,202 + Pf6N2E2_506 0.56 +1.8 575,202 + Pf6N2E2_506 0.56 -0.2 575,218 + Pf6N2E2_506 0.61 -0.1 575,219 - Pf6N2E2_506 0.62 +0.7 575,219 - Pf6N2E2_506 0.62 -0.3 575,219 - Pf6N2E2_506 0.62 +0.4 575,219 - Pf6N2E2_506 0.62 +0.1 575,219 - Pf6N2E2_506 0.62 -0.3 575,498 + Pf6N2E2_507 0.11 +1.2
Or see this region's nucleotide sequence