Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5001

Experiment: pyrophosphate 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5000 and Pf6N2E2_5001 are separated by 168 nucleotidesPf6N2E2_5001 and Pf6N2E2_5002 are separated by 81 nucleotidesPf6N2E2_5002 and Pf6N2E2_5003 overlap by 1 nucleotides Pf6N2E2_5000 - Rare lipoprotein A precursor, at 5,766,197 to 5,767,222 _5000 Pf6N2E2_5001 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 5,767,391 to 5,768,548 _5001 Pf6N2E2_5002 - Proposed lipoate regulatory protein YbeD, at 5,768,630 to 5,768,905 _5002 Pf6N2E2_5003 - Octanoate-[acyl-carrier-protein]-protein-N-octan oyltransferase, at 5,768,905 to 5,769,552 _5003 Position (kb) 5767 5768 5769Strain fitness (log2 ratio) -2 -1 0 1 2at 5767.275 kb on + strandat 5767.275 kb on + strandat 5767.311 kb on + strandat 5767.311 kb on + strandat 5767.311 kb on + strandat 5767.312 kb on - strandat 5767.312 kb on - strandat 5767.602 kb on - strand, within Pf6N2E2_5001at 5767.904 kb on + strand, within Pf6N2E2_5001at 5768.231 kb on + strand, within Pf6N2E2_5001at 5768.491 kb on + strandat 5768.531 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M
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5,767,275 + +0.8
5,767,275 + -0.8
5,767,311 + +0.1
5,767,311 + +0.8
5,767,311 + +1.1
5,767,312 - +1.8
5,767,312 - -0.3
5,767,602 - Pf6N2E2_5001 0.18 +0.1
5,767,904 + Pf6N2E2_5001 0.44 -1.8
5,768,231 + Pf6N2E2_5001 0.73 +1.3
5,768,491 + +0.5
5,768,531 + +0.2

Or see this region's nucleotide sequence