Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4270 and Pf6N2E2_4271 are separated by 13 nucleotides Pf6N2E2_4271 and Pf6N2E2_4272 are separated by 140 nucleotides Pf6N2E2_4272 and Pf6N2E2_4273 are separated by 311 nucleotides
Pf6N2E2_4270 - putative Glutathione-regulated potassium-efflux system protein KefB, at 4,966,890 to 4,968,653
_4270
Pf6N2E2_4271 - FIG00956226: hypothetical protein, at 4,968,667 to 4,969,074
_4271
Pf6N2E2_4272 - putative membrane protein, at 4,969,215 to 4,970,093
_4272
Pf6N2E2_4273 - ATP-dependent DNA helicase UvrD/PcrA, at 4,970,405 to 4,972,588
_4273
Position (kb)
4969
4970
4971 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4968.632 kb on + strand at 4968.632 kb on + strand at 4968.632 kb on + strand at 4968.632 kb on + strand at 4968.632 kb on + strand at 4968.782 kb on + strand, within Pf6N2E2_4271 at 4968.782 kb on + strand, within Pf6N2E2_4271 at 4968.782 kb on + strand, within Pf6N2E2_4271 at 4968.782 kb on + strand, within Pf6N2E2_4271 at 4968.783 kb on - strand, within Pf6N2E2_4271 at 4968.783 kb on - strand, within Pf6N2E2_4271 at 4968.783 kb on - strand, within Pf6N2E2_4271 at 4968.785 kb on + strand, within Pf6N2E2_4271 at 4968.785 kb on + strand, within Pf6N2E2_4271 at 4968.785 kb on + strand, within Pf6N2E2_4271 at 4968.785 kb on + strand, within Pf6N2E2_4271 at 4968.785 kb on + strand, within Pf6N2E2_4271 at 4968.786 kb on - strand, within Pf6N2E2_4271 at 4968.786 kb on - strand, within Pf6N2E2_4271 at 4968.786 kb on - strand, within Pf6N2E2_4271 at 4968.786 kb on - strand, within Pf6N2E2_4271 at 4968.806 kb on - strand, within Pf6N2E2_4271 at 4968.866 kb on + strand, within Pf6N2E2_4271 at 4968.866 kb on + strand, within Pf6N2E2_4271 at 4968.866 kb on + strand, within Pf6N2E2_4271 at 4968.867 kb on - strand, within Pf6N2E2_4271 at 4968.867 kb on - strand, within Pf6N2E2_4271 at 4968.867 kb on - strand, within Pf6N2E2_4271 at 4968.914 kb on + strand, within Pf6N2E2_4271 at 4968.914 kb on + strand, within Pf6N2E2_4271 at 4968.915 kb on - strand, within Pf6N2E2_4271 at 4969.108 kb on + strand at 4969.108 kb on + strand at 4969.108 kb on + strand at 4969.109 kb on - strand at 4969.109 kb on - strand at 4969.109 kb on - strand at 4969.110 kb on + strand at 4969.110 kb on + strand at 4969.110 kb on + strand at 4969.111 kb on - strand at 4969.111 kb on - strand at 4969.111 kb on - strand at 4969.122 kb on + strand at 4969.122 kb on + strand at 4969.122 kb on + strand at 4969.123 kb on - strand at 4969.123 kb on - strand at 4969.124 kb on + strand at 4969.124 kb on + strand at 4969.124 kb on + strand at 4969.124 kb on + strand at 4969.124 kb on + strand at 4969.124 kb on + strand at 4969.124 kb on + strand at 4969.125 kb on - strand at 4969.125 kb on - strand at 4969.125 kb on - strand at 4969.125 kb on - strand at 4969.125 kb on - strand at 4969.125 kb on - strand at 4969.125 kb on - strand at 4969.129 kb on + strand at 4969.129 kb on + strand at 4969.129 kb on + strand at 4969.129 kb on + strand at 4969.129 kb on + strand at 4969.129 kb on + strand at 4969.130 kb on - strand at 4969.130 kb on - strand at 4969.130 kb on - strand at 4969.130 kb on - strand at 4969.130 kb on - strand at 4969.130 kb on - strand at 4969.130 kb on - strand at 4969.137 kb on + strand at 4969.138 kb on - strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.139 kb on + strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.140 kb on - strand at 4969.157 kb on - strand at 4969.188 kb on - strand at 4969.412 kb on - strand, within Pf6N2E2_4272 at 4969.412 kb on - strand, within Pf6N2E2_4272 at 4969.412 kb on - strand, within Pf6N2E2_4272 at 4969.412 kb on - strand, within Pf6N2E2_4272 at 4969.412 kb on - strand, within Pf6N2E2_4272 at 4970.003 kb on + strand, within Pf6N2E2_4272 at 4970.004 kb on - strand, within Pf6N2E2_4272 at 4970.004 kb on - strand, within Pf6N2E2_4272 at 4970.078 kb on + strand at 4970.079 kb on - strand at 4970.079 kb on - strand at 4970.093 kb on + strand at 4970.093 kb on + strand at 4970.094 kb on - strand at 4970.094 kb on - strand at 4970.094 kb on - strand at 4970.094 kb on - strand at 4970.095 kb on + strand at 4970.095 kb on + strand at 4970.095 kb on + strand at 4970.096 kb on - strand at 4970.096 kb on - strand at 4970.193 kb on + strand at 4970.239 kb on + strand at 4970.248 kb on + strand at 4970.248 kb on + strand at 4970.249 kb on - strand at 4970.252 kb on + strand at 4970.252 kb on + strand at 4970.252 kb on + strand at 4970.252 kb on + strand at 4970.253 kb on - strand at 4970.253 kb on - strand at 4970.253 kb on - strand at 4970.269 kb on + strand at 4970.269 kb on + strand at 4970.269 kb on + strand at 4970.269 kb on + strand at 4970.269 kb on + strand at 4970.269 kb on + strand at 4970.270 kb on - strand at 4970.270 kb on - strand at 4970.270 kb on - strand at 4970.270 kb on - strand at 4970.270 kb on - strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.288 kb on + strand at 4970.289 kb on - strand at 4970.289 kb on - strand at 4970.289 kb on - strand at 4970.289 kb on - strand at 4970.289 kb on - strand at 4970.289 kb on - strand at 4970.395 kb on + strand at 4970.395 kb on + strand at 4970.396 kb on - strand at 4970.428 kb on - strand at 4970.675 kb on + strand, within Pf6N2E2_4273 at 4970.684 kb on + strand, within Pf6N2E2_4273 at 4970.684 kb on + strand, within Pf6N2E2_4273 at 4970.684 kb on + strand, within Pf6N2E2_4273 at 4970.685 kb on - strand, within Pf6N2E2_4273 at 4970.685 kb on - strand, within Pf6N2E2_4273 at 4970.685 kb on - strand, within Pf6N2E2_4273 at 4970.706 kb on + strand, within Pf6N2E2_4273 at 4970.706 kb on + strand, within Pf6N2E2_4273 at 4970.707 kb on - strand, within Pf6N2E2_4273 at 4970.721 kb on + strand, within Pf6N2E2_4273 at 4970.721 kb on + strand, within Pf6N2E2_4273 at 4970.721 kb on + strand, within Pf6N2E2_4273 at 4970.722 kb on - strand, within Pf6N2E2_4273 at 4970.722 kb on - strand, within Pf6N2E2_4273 at 4970.722 kb on - strand, within Pf6N2E2_4273 at 4970.742 kb on + strand, within Pf6N2E2_4273 at 4970.743 kb on - strand, within Pf6N2E2_4273
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 4,968,632 + -0.1 4,968,632 + -0.4 4,968,632 + -0.4 4,968,632 + -1.0 4,968,632 + -0.6 4,968,782 + Pf6N2E2_4271 0.28 -0.7 4,968,782 + Pf6N2E2_4271 0.28 -1.6 4,968,782 + Pf6N2E2_4271 0.28 -2.7 4,968,782 + Pf6N2E2_4271 0.28 -0.0 4,968,783 - Pf6N2E2_4271 0.28 +0.6 4,968,783 - Pf6N2E2_4271 0.28 -1.0 4,968,783 - Pf6N2E2_4271 0.28 -0.3 4,968,785 + Pf6N2E2_4271 0.29 -1.1 4,968,785 + Pf6N2E2_4271 0.29 -0.4 4,968,785 + Pf6N2E2_4271 0.29 -0.3 4,968,785 + Pf6N2E2_4271 0.29 +0.3 4,968,785 + Pf6N2E2_4271 0.29 +2.2 4,968,786 - Pf6N2E2_4271 0.29 -0.0 4,968,786 - Pf6N2E2_4271 0.29 -0.7 4,968,786 - Pf6N2E2_4271 0.29 +0.3 4,968,786 - Pf6N2E2_4271 0.29 +0.2 4,968,806 - Pf6N2E2_4271 0.34 -0.4 4,968,866 + Pf6N2E2_4271 0.49 -0.4 4,968,866 + Pf6N2E2_4271 0.49 +0.2 4,968,866 + Pf6N2E2_4271 0.49 +1.4 4,968,867 - Pf6N2E2_4271 0.49 -0.2 4,968,867 - Pf6N2E2_4271 0.49 -0.3 4,968,867 - Pf6N2E2_4271 0.49 -0.6 4,968,914 + Pf6N2E2_4271 0.61 -0.6 4,968,914 + Pf6N2E2_4271 0.61 +0.9 4,968,915 - Pf6N2E2_4271 0.61 -0.0 4,969,108 + -0.4 4,969,108 + +0.1 4,969,108 + -0.2 4,969,109 - +0.6 4,969,109 - -0.6 4,969,109 - -1.3 4,969,110 + -0.9 4,969,110 + +0.9 4,969,110 + -1.1 4,969,111 - -0.5 4,969,111 - +0.2 4,969,111 - +0.8 4,969,122 + +1.0 4,969,122 + +0.3 4,969,122 + +0.9 4,969,123 - -0.2 4,969,123 - -1.2 4,969,124 + -1.0 4,969,124 + -1.0 4,969,124 + +0.2 4,969,124 + -0.9 4,969,124 + -1.1 4,969,124 + +1.6 4,969,124 + -0.5 4,969,125 - -1.3 4,969,125 - +0.9 4,969,125 - -1.6 4,969,125 - -0.1 4,969,125 - +1.4 4,969,125 - -0.8 4,969,125 - +0.8 4,969,129 + -0.6 4,969,129 + +0.0 4,969,129 + +0.3 4,969,129 + -0.4 4,969,129 + +0.1 4,969,129 + -0.1 4,969,130 - -0.6 4,969,130 - +0.3 4,969,130 - -0.1 4,969,130 - -0.5 4,969,130 - -0.3 4,969,130 - +0.4 4,969,130 - -1.6 4,969,137 + -1.1 4,969,138 - -0.6 4,969,139 + -1.6 4,969,139 + -1.6 4,969,139 + +0.5 4,969,139 + -0.2 4,969,139 + -0.8 4,969,139 + +0.4 4,969,139 + +0.0 4,969,139 + -0.5 4,969,140 - +0.3 4,969,140 - -0.9 4,969,140 - -1.0 4,969,140 - +0.3 4,969,140 - -3.0 4,969,140 - -0.2 4,969,140 - -0.6 4,969,140 - -0.2 4,969,157 - -0.8 4,969,188 - +0.0 4,969,412 - Pf6N2E2_4272 0.22 -0.6 4,969,412 - Pf6N2E2_4272 0.22 -1.2 4,969,412 - Pf6N2E2_4272 0.22 +0.8 4,969,412 - Pf6N2E2_4272 0.22 -1.1 4,969,412 - Pf6N2E2_4272 0.22 -0.3 4,970,003 + Pf6N2E2_4272 0.90 -0.2 4,970,004 - Pf6N2E2_4272 0.90 -2.0 4,970,004 - Pf6N2E2_4272 0.90 -0.6 4,970,078 + -2.2 4,970,079 - +1.5 4,970,079 - -0.4 4,970,093 + +0.2 4,970,093 + -0.4 4,970,094 - +1.1 4,970,094 - +0.5 4,970,094 - -0.5 4,970,094 - +0.7 4,970,095 + -1.2 4,970,095 + +0.4 4,970,095 + -1.8 4,970,096 - -0.4 4,970,096 - +0.5 4,970,193 + -1.0 4,970,239 + -0.2 4,970,248 + +0.2 4,970,248 + -0.3 4,970,249 - -0.3 4,970,252 + +0.1 4,970,252 + -0.6 4,970,252 + +0.5 4,970,252 + -0.2 4,970,253 - -0.2 4,970,253 - -1.0 4,970,253 - -0.1 4,970,269 + +0.2 4,970,269 + -0.2 4,970,269 + -0.4 4,970,269 + +0.8 4,970,269 + +0.8 4,970,269 + +0.0 4,970,270 - -0.5 4,970,270 - -0.9 4,970,270 - -0.5 4,970,270 - +0.6 4,970,270 - +0.4 4,970,288 + +0.5 4,970,288 + -0.6 4,970,288 + -0.5 4,970,288 + -0.1 4,970,288 + +0.0 4,970,288 + -1.8 4,970,288 + -1.3 4,970,288 + -0.2 4,970,288 + -0.6 4,970,289 - +0.2 4,970,289 - +1.3 4,970,289 - +0.7 4,970,289 - +0.5 4,970,289 - -0.0 4,970,289 - -1.3 4,970,395 + +1.1 4,970,395 + +0.8 4,970,396 - +0.1 4,970,428 - -0.6 4,970,675 + Pf6N2E2_4273 0.12 -0.3 4,970,684 + Pf6N2E2_4273 0.13 -0.3 4,970,684 + Pf6N2E2_4273 0.13 -0.6 4,970,684 + Pf6N2E2_4273 0.13 +0.6 4,970,685 - Pf6N2E2_4273 0.13 +1.1 4,970,685 - Pf6N2E2_4273 0.13 +1.2 4,970,685 - Pf6N2E2_4273 0.13 -0.6 4,970,706 + Pf6N2E2_4273 0.14 +0.4 4,970,706 + Pf6N2E2_4273 0.14 -1.7 4,970,707 - Pf6N2E2_4273 0.14 +0.0 4,970,721 + Pf6N2E2_4273 0.14 -0.6 4,970,721 + Pf6N2E2_4273 0.14 -1.0 4,970,721 + Pf6N2E2_4273 0.14 -0.9 4,970,722 - Pf6N2E2_4273 0.15 +0.4 4,970,722 - Pf6N2E2_4273 0.15 +1.0 4,970,722 - Pf6N2E2_4273 0.15 +0.4 4,970,742 + Pf6N2E2_4273 0.15 +0.8 4,970,743 - Pf6N2E2_4273 0.15 +0.0
Or see this region's nucleotide sequence