Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_337 and Pf6N2E2_338 are separated by 147 nucleotides Pf6N2E2_338 and Pf6N2E2_339 are separated by 6 nucleotides Pf6N2E2_339 and Pf6N2E2_340 are separated by 1 nucleotides Pf6N2E2_340 and Pf6N2E2_341 are separated by 177 nucleotides
Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859
_337
Pf6N2E2_338 - Transcriptional regulator, AraC family, at 405,007 to 405,510
_338
Pf6N2E2_339 - Glutathione-regulated potassium-efflux system protein KefC, at 405,517 to 407,343
_339
Pf6N2E2_340 - Glutathione-regulated potassium-efflux system ancillary protein KefF, at 407,345 to 407,866
_340
Pf6N2E2_341 - sensor histidine kinase, at 408,044 to 409,390
_341
Position (kb) 405
406
407
408 Strain fitness (log2 ratio) -2
-1
0
1
2 at 404.536 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.537 kb on - strand at 404.669 kb on - strand, within Pf6N2E2_337 at 404.875 kb on + strand at 404.875 kb on + strand at 404.876 kb on - strand at 405.098 kb on + strand, within Pf6N2E2_338 at 405.098 kb on + strand, within Pf6N2E2_338 at 405.732 kb on - strand, within Pf6N2E2_339 at 405.732 kb on - strand, within Pf6N2E2_339 at 406.027 kb on - strand, within Pf6N2E2_339 at 406.064 kb on - strand, within Pf6N2E2_339 at 407.188 kb on + strand at 407.189 kb on - strand at 407.189 kb on - strand at 407.239 kb on + strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.243 kb on + strand at 407.244 kb on - strand at 407.309 kb on + strand at 407.309 kb on + strand at 407.309 kb on + strand at 407.310 kb on - strand at 407.310 kb on - strand at 407.310 kb on - strand at 407.379 kb on + strand at 407.379 kb on + strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.421 kb on + strand, within Pf6N2E2_340 at 407.421 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.664 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.752 kb on - strand, within Pf6N2E2_340 at 407.760 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.838 kb on + strand at 407.838 kb on + strand at 407.850 kb on + strand at 407.851 kb on - strand at 407.851 kb on - strand at 407.940 kb on + strand at 408.018 kb on + strand at 408.316 kb on - strand, within Pf6N2E2_341 at 408.316 kb on - strand, within Pf6N2E2_341 at 408.316 kb on - strand, within Pf6N2E2_341
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 404,536 + -1.1 404,536 + -0.8 404,536 + +1.2 404,537 - +0.3 404,669 - Pf6N2E2_337 0.30 -0.6 404,875 + -0.1 404,875 + -0.8 404,876 - +0.3 405,098 + Pf6N2E2_338 0.18 -0.5 405,098 + Pf6N2E2_338 0.18 +0.0 405,732 - Pf6N2E2_339 0.12 -1.4 405,732 - Pf6N2E2_339 0.12 -1.7 406,027 - Pf6N2E2_339 0.28 -1.6 406,064 - Pf6N2E2_339 0.30 -0.3 407,188 + -0.3 407,189 - +0.7 407,189 - -0.2 407,239 + +1.4 407,240 - +0.2 407,240 - +0.0 407,240 - -1.7 407,240 - +0.8 407,240 - -1.8 407,243 + +0.4 407,244 - -2.2 407,309 + -0.1 407,309 + +0.2 407,309 + -0.5 407,310 - -0.3 407,310 - -1.6 407,310 - +1.0 407,379 + +1.0 407,379 + +2.2 407,380 - +0.2 407,380 - -0.4 407,380 - +0.3 407,380 - -1.0 407,380 - +1.0 407,421 + Pf6N2E2_340 0.15 -0.2 407,421 + Pf6N2E2_340 0.15 +0.4 407,466 + Pf6N2E2_340 0.23 -0.4 407,466 + Pf6N2E2_340 0.23 +0.3 407,466 + Pf6N2E2_340 0.23 +1.8 407,664 + Pf6N2E2_340 0.61 +0.7 407,670 + Pf6N2E2_340 0.62 -0.6 407,670 + Pf6N2E2_340 0.62 +0.5 407,670 + Pf6N2E2_340 0.62 -0.4 407,752 - Pf6N2E2_340 0.78 +1.1 407,760 + Pf6N2E2_340 0.80 -0.1 407,771 + Pf6N2E2_340 0.82 +0.9 407,771 + Pf6N2E2_340 0.82 -2.6 407,771 + Pf6N2E2_340 0.82 +0.4 407,772 - Pf6N2E2_340 0.82 +0.0 407,772 - Pf6N2E2_340 0.82 +2.0 407,772 - Pf6N2E2_340 0.82 +1.6 407,838 + -0.2 407,838 + +0.7 407,850 + +0.2 407,851 - -0.7 407,851 - +0.8 407,940 + -0.4 408,018 + -0.2 408,316 - Pf6N2E2_341 0.20 -0.3 408,316 - Pf6N2E2_341 0.20 +0.4 408,316 - Pf6N2E2_341 0.20 -0.0
Or see this region's nucleotide sequence