Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_225 and Pf6N2E2_226 are separated by 74 nucleotides Pf6N2E2_226 and Pf6N2E2_227 are separated by 22 nucleotides
Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783
_225
Pf6N2E2_226 - 2,3-dihydroxybiphenyl 1,2-dioxygenase, at 287,858 to 288,706
_226
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Position (kb) 287
288
289 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.865 kb on - strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.617 kb on + strand, within Pf6N2E2_225 at 287.618 kb on - strand, within Pf6N2E2_225 at 287.737 kb on + strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.792 kb on - strand at 287.821 kb on - strand at 287.821 kb on - strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 288.109 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.362 kb on + strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.666 kb on + strand at 288.730 kb on + strand at 289.034 kb on + strand, within Pf6N2E2_227 at 289.035 kb on - strand, within Pf6N2E2_227 at 289.237 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 286,864 + Pf6N2E2_225 0.38 +0.1 286,864 + Pf6N2E2_225 0.38 +0.4 286,865 - Pf6N2E2_225 0.38 -0.3 287,377 + Pf6N2E2_225 0.72 +0.5 287,377 + Pf6N2E2_225 0.72 +0.2 287,377 + Pf6N2E2_225 0.72 -1.0 287,617 + Pf6N2E2_225 0.89 +0.5 287,618 - Pf6N2E2_225 0.89 -0.3 287,737 + -1.2 287,738 - -1.9 287,738 - -0.4 287,738 - +0.0 287,738 - -1.1 287,738 - -0.2 287,792 - -0.1 287,821 - +0.2 287,821 - -0.8 287,898 + -0.5 287,898 + +1.9 287,898 + -0.1 287,899 - -1.1 287,899 - -0.4 287,899 - +0.5 287,899 - -3.4 287,899 - -0.2 288,109 - Pf6N2E2_226 0.30 -1.1 288,256 - Pf6N2E2_226 0.47 -0.3 288,256 - Pf6N2E2_226 0.47 -0.4 288,362 + Pf6N2E2_226 0.59 -0.2 288,363 - Pf6N2E2_226 0.59 -1.0 288,363 - Pf6N2E2_226 0.59 -2.4 288,666 + -0.5 288,730 + +0.5 289,034 + Pf6N2E2_227 0.19 +0.3 289,035 - Pf6N2E2_227 0.19 +0.1 289,237 + Pf6N2E2_227 0.31 -0.6 289,489 + Pf6N2E2_227 0.46 -0.2 289,489 + Pf6N2E2_227 0.46 -0.3 289,574 - Pf6N2E2_227 0.51 +0.2 289,574 - Pf6N2E2_227 0.51 +0.3 289,574 - Pf6N2E2_227 0.51 -0.6 289,574 - Pf6N2E2_227 0.51 +0.7
Or see this region's nucleotide sequence